Gene - combatTB X

ppsE

Type: protein_coding
Name: ppsE
Locus Name: Rv2935
Product: Phenolpthiocerol synthesis type-I polyketide synthase PpsE
Functional Category: Lipid metabolism
Location: 3267737..3272203 (+ strand)
Gene Length: 4466 bp
Nucleotides: TGAGCATCCCCGAGAACGCGATCGCGGTGGTCGGCATGGCCGGCCGATTTCCGGGCGCCAAGGATGTTTCGGCGTTCTGGAGCAACCTTCGGCGCGGTAAGGAGTCGATCGTCACCCTGTCCGAACAGGAGCTGCGCGACGCCGGCGTCAGCGACAAGACGCTGGCCGATCCGGCGTATGTGCGTCGCGCCCCGCTTCTTGACGGGATCGACGAGTTCGACGCCGGCTTCTTCGGGTTCCCGCCGCTGGCCGCGCAGGTGCTGGATCCCCAACACCGGTTGTTCCTGCAGTGTGCATGGCATGCGCTCGAGGACGCGGGCGCTGACCCCGCACGGTTCGACGGCTCGATCGGCGTATACGGAACCAGCTCCCCCAGCGGCTATCTGCTGCACAACCTGCTGTCGCATCGCGACCCGAACGCTGTGTTGGCCGAGGGACTCAACTTCGACCAGTTCAGCCTGTTCTTGCAGAATGACAAGGACTTTCTGGCAACCCGGATTTCGCACGCGTTCAACCTGCGCGGGCCGAGCATCGCGGTGCAAACCGCGTGTTCATCGTCGCTGGTAGCGGTGCATCTGGCCTGCCTGAGCCTGCTATCCGGCGAATGCGACATGGCGTTGGCCGGCGGGTCGTCGCTATGCATCCCGCACCGTGTCGGCTACTTCACCTCACCGGGATCGATGGTGTCGGCGGTGGGCCACTGTCGGCCCTTCGACGTGCGGGCCGACGGCACGGTCTTCGGCAGCGGTGTCGGGTTGGTGGTGCTCAAGCCGCTGGCGGCCGCCATCGACGCCGGAGACCGGATTCACGCCGTCATCCGCGGATCGGCGATCAACAACGACGGATCGGCGAAGATGGGGTATGCGGCGCCCAACCCGGCCGCTCAAGCCGATGTCATCGCCGAAGCCCATGCGGTGTCCGGCATCGATTCGTCGACCGTGAGCTATGTCGAGTGCCACGGAACCGGCACCCCGCTCGGTGATCCTATCGAAATCCAGGGCCTGCGAGCGGCGTTCGAGGTGTCGCAGACGAGCCGTTCGGCCCCTTGTGTTCTGGGGTCGGTCAAGTCGAACATCGGCCACCTGGAAGTTGCTGCCGGCATCGCGGGTCTGATCAAAACGATTCTGTGCCTAAAGAACAAGGCACTACCCGCGACGCTGCACTACACCAGCCCGAACCCGGAACTGCGCTTGGACCAAAGTCCGTTCGTCGTGCAAAGCAAGTACGGCCCCTGGGAGTGCGACGGCGTTCGTCGTGCCGGGGTGAGTTCGTTCGGGGTCGGGGGTACCAACGCGCACGTCGTCTTGGAGGAGGCGCCAGCAGAAGCATCGGAGGTTTCAGCGCACGCCGAGCCGGCTGGCCCTCAGGTAATCCTGCTCTCGGCGCAAACGGCCGCGGCGCTCGGCGAGTCGCGGACCGCCCTGGCCGCGGCGCTAGAAACGCAAGACGGCCCGCGCCTGTCCGACGTGGCCTACACGCTCGCCCGGCGCCGCAAGCACAACGTCACGATGGCCGCCGTCGTGCACGACCGCGAGCACGCGGCCACCGTGCTGCGGGCGGCCGAGCACGACAACGTTTTCGTTGGCGAAGCCGCCCACGATGGGGAGCATGGCGATCGCGCCGACGCCGCACCCACGTCGGATCGCGTCGTTTTCCTGTTTCCCGGACAGGGCGCTCAGCACGTCGGAATGGCAAAAGGGCTCTATGACACCGAGCCGGTCTTCGCCCAACACTTCGACACCTGCGCCGCCGGATTCCGCGACGAGACAGGCATCGACTTGCATGCCGAAGTGTTCGACGGGACCGCAACAGATCTTGAGCGCATTGACCGTTCGCAACCGGCATTGTTCACGGTGGAATACGCGCTCGCGAAGTTGGTCGACACTTTCGGCGTGCGCGCCGGGGCGTACATCGGATACAGCACCGGCGAATACATCGCGGCCACCCTGGCCGGCGTATTCGACCTGCAGACAGCGATCAAAACGGTGTCGCTGCGCGCCCGCCTTATGCATGAGTCGCCGCCCGGTGCCATGGTCGCGGTGGCTCTTGGCCCCGATGACGTCACGCAGTACCTGCCACCGGAGGTCGAGCTGTCCGCGGTAAACGATCCTGGTAACTGTGTGGTCGCCGGGCCCAAAGACCAGATCCGTGCACTGCGCCAACGTCTTACCGAGGCAGGGATTCCCGTTCGCCGCGTCCGGGCAACCCACGCGTTCCATACCAGCGCGATGGATCCCATGCTGGGCCAATTCCAAGAATTCCTGTCCCGTCAACAGCTACGTCCTCCGCGCACACCGCTGCTGAGCAACCTCACCGGTAGCTGGATGTCCGACCAGCAAGTAGTCGATCCGGCCAGCTGGACGCGTCAAATCAGCTCCCCCATCAGGTTCGCCGACGAGCTGGACGTGGTGCTGGCAGCTCCAAGTCGAATCCTGGTCGAGGTTGGTCCGGGCGGCAGCCTGACCGGTTCGGCTATGCGCCACCCGAAGTGGTCGACCACGCACCGCACCGTTCGGCTTATGCGCCACCCACTGCAAGACGTCGACGACCGCGACACTTTTCTGCGCGCGCTGGGCGAACTCTGGTCTGCCGGAGTCGAGGTCGACTGGACGCCGCGGCGTCCGGCGGTGCCGCACCTCGTTTCCCTGCCGGGTTATCCATTTGCCCGTCAACGGCATTGGGTCGAACCTAACCACACGGTTTGGGCGCAGGCTCCCGGCGCAAACAACGGCTCACCGGCCGGCACTGCGGATGGTTCCACGGCCGCCACCGTCGATGCAGCCCGCAACGGAGAGTCGCAGACCGAGGTTACGCTGCAACGCATCTGGTCACAGTGCCTCGGCGTCAGCTCGGTCGATCGGAACGCCAATTTCTTCGACCTCGGCGGCGATTCTTTGATGGCGATCAGCATCGCGATGGCCGCCGCCAACGAGGGTCTGACCATCACGCCGCAGGATCTCTACGAATACCCGACCCTGGCCTCGCTGACGGCCGCCGTCGACGCGTCGTTCGCGTCCAGCGGGTTGGCGAAGCCCCCGGAGGCACAAGCGAACCCGGCGGTTCCACCCAACGTCACGTACTTCCTCGACCGCGGATTGCGCGACACCGGCCGCTGTCGTGTCCCGCTGATCCTGCGCCTGGATCCCAAGATCGGGCTACCGGATATTCGAGCGGTGCTGACCGCAGTGGTCAACCACCACGACGCATTGCGCCTGCACCTGGTCGGCAACGATGGGATATGGGAGCAGCACATCGCGGCACCCGCAGAATTCACCGGGCTTTCCAACCGGTCGGTGCCCAACGGCGTGGCTGCAGGCAGCCCCGAGGAACGGGCCGCGGTCTTGGGCATCCTGGCCGAACTCCTTGAGGATCAAACGGATCCGAACGCGCCGCTGGCTGCCGTTCATATCGCCGCCGCGCACGGCGGTCCGCACTATCTGTGCCTTGCCATACATGCGATGGTCACCGACGACTCATCGCGCCAGATCCTGGCGACCGACATCGTCACCGCGTTTGGACAACGGCTGGCAGGCGAGGAGATCACGCTGGAACCGGTCAGCACGGGGTGGCGGGAATGGTCACTGCGTTGCGCGGCCCTCGCGACGCATCCGGCGGCGCTGGACACTCGCTCGTACTGGATCGAGAATTCGACCAAGGCGACTTTGTGGCTGGCCGATGCCCTTCCCAACGCGCATACCGCCCATCCGCCCCGCGCCGACGAGCTCACCAAGTTGTCGAGCACGCTAAGCGTCGAGCAGACATCCGAGCTGGACGACGGCCGGCGCAGGTTCCGCCGGTCGATTCAGACGATCCTGCTGGCCGCCCTCGGCCGCACAATAGCTCAGACGGTAGGTGAGGGTGTGGTCGCCGTGGAGCTCGAAGGCGAGGGCCGCTCGGTGCTGCGGCCGGATGTCGACCTGCGCAGAACGGTCGGCTGGTTCACGACGTACTACCCGGTACCGCTGGCATGCGCAACAGGGCTGGGCGCGCTTGCGCAGCTGGACGCGGTGCACAACACTCTTAAGTCCGTTCCGCACTACGGAATTGGATACGGGCTGCTGCGCTACGTTTACGCCCCGACCGGACGTGTCCTGGGCGCTCAGCGCACACCCGACATTCACTTCCGGTATGCGGGCGTGATCCCCGAGCTACCGTCCGGCGATGCTCCAGTACAGTTCGACTCGGACATGACGCTTCCGGTGCGCGAACCGATCCCAGGGATGGGCCACGCCATCGAACTTCGGGTGTATCGGTTTGGTGGCTCACTGCATCTCGATTGGTGGTACGACACCCGCCGGATCCCGGCGGCAACGGCAGAAGCGCTGGAGCGGACCTTCCCGCTGGCCCTCAGCGCGCTGATCCAGGAGGCCATCGCGGCCGAGCACACAGAGCACGACGACAGCGAGATAGTCGGGGAACCCGAGGCGGGCGCTCTGGTGGACCTGTCGAGCATGGATGCCGGCTGA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Involved in the elongation of either C22-24 fatty acids by the addition of malonyl-CoA and methylmalonyl-CoA extender units to yield phthiocerol derivatives. {ECO:0000269|PubMed:19197369}.

Family

Unknown

GO

Actinobacterium-type cell wall biogenesis GO:0071766 biological_process
DIM/DIP cell wall layer assembly GO:0071770 biological_process
polyketide synthase complex GO:0034081 cellular_component
lipid biosynthetic process GO:0008610 biological_process
cell wall GO:0005618 cellular_component
phosphopantetheine binding GO:0031177 molecular_function
plasma membrane GO:0005886 cellular_component
oxidoreductase activity GO:0016491 molecular_function
3-oxoacyl-[acyl-carrier-protein] synthase activity GO:0004315 molecular_function
fatty acid biosynthetic process GO:0006633 biological_process

InterPro

UniProt: P9WQE1
GenBank: Rv2935
EnsemblBacteria: Rv2935
Mycobrowser: Rv2935

Summary

Name: Phthiocerol synthesis polyketide synthase type I PpsE (Beta-ketoacyl-acyl-carrier-protein synthase I) (EC 2.3.1.41)
Family: Unknown
Protein Sequence: MSIPENAIAVVGMAGRFPGAKDVSAFWSNLRRGKESIVTLSEQELRDAGVSDKTLADPAYVRRAPLLDGIDEFDAGFFGFPPLAAQVLDPQHRLFLQCAWHALEDAGADPARFDGSIGVYGTSSPSGYLLHNLLSHRDPNAVLAEGLNFDQFSLFLQNDKDFLATRISHAFNLRGPSIAVQTACSSSLVAVHLACLSLLSGECDMALAGGSSLCIPHRVGYFTSPGSMVSAVGHCRPFDVRADGTVFGSGVGLVVLKPLAAAIDAGDRIHAVIRGSAINNDGSAKMGYAAPNPAAQADVIAEAHAVSGIDSSTVSYVECHGTGTPLGDPIEIQGLRAAFEVSQTSRSAPCVLGSVKSNIGHLEVAAGIAGLIKTILCLKNKALPATLHYTSPNPELRLDQSPFVVQSKYGPWECDGVRRAGVSSFGVGGTNAHVVLEEAPAEASEVSAHAEPAGPQVILLSAQTAAALGESRTALAAALETQDGPRLSDVAYTLARRRKHNVTMAAVVHDREHAATVLRAAEHDNVFVGEAAHDGEHGDRADAAPTSDRVVFLFPGQGAQHVGMAKGLYDTEPVFAQHFDTCAAGFRDETGIDLHAEVFDGTATDLERIDRSQPALFTVEYALAKLVDTFGVRAGAYIGYSTGEYIAATLAGVFDLQTAIKTVSLRARLMHESPPGAMVAVALGPDDVTQYLPPEVELSAVNDPGNCVVAGPKDQIRALRQRLTEAGIPVRRVRATHAFHTSAMDPMLGQFQEFLSRQQLRPPRTPLLSNLTGSWMSDQQVVDPASWTRQISSPIRFADELDVVLAAPSRILVEVGPGGSLTGSAMRHPKWSTTHRTVRLMRHPLQDVDDRDTFLRALGELWSAGVEVDWTPRRPAVPHLVSLPGYPFARQRHWVEPNHTVWAQAPGANNGSPAGTADGSTAATVDAARNGESQTEVTLQRIWSQCLGVSSVDRNANFFDLGGDSLMAISIAMAAANEGLTITPQDLYEYPTLASLTAAVDASFASSGLAKPPEAQANPAVPPNVTYFLDRGLRDTGRCRVPLILRLDPKIGLPDIRAVLTAVVNHHDALRLHLVGNDGIWEQHIAAPAEFTGLSNRSVPNGVAAGSPEERAAVLGILAELLEDQTDPNAPLAAVHIAAAHGGPHYLCLAIHAMVTDDSSRQILATDIVTAFGQRLAGEEITLEPVSTGWREWSLRCAALATHPAALDTRSYWIENSTKATLWLADALPNAHTAHPPRADELTKLSSTLSVEQTSELDDGRRRFRRSIQTILLAALGRTIAQTVGEGVVAVELEGEGRSVLRPDVDLRRTVGWFTTYYPVPLACATGLGALAQLDAVHNTLKSVPHYGIGYGLLRYVYAPTGRVLGAQRTPDIHFRYAGVIPELPSGDAPVQFDSDMTLPVREPIPGMGHAIELRVYRFGGSLHLDWWYDTRRIPAATAEALERTFPLALSALIQEAIAAEHTEHDDSEIVGEPEAGALVDLSSMDAG
Mass: 158,745 Da
Length: 1488 Aa

Rv2935 doesn't seem to be a targeted by any drug.

Rv2935 doesn't seem to be involved in any pathway.

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Phthiocerol dimycocerosates of M. tuberculosis participate in macrophage invasion by inducing changes in the organization of plasma membrane lipids.: PLoS Pathog. 2009 Feb;5(2):e1000289. doi: 10.1371/journal.ppat.1000289. Epub 2009 Feb 6.
targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis.: BMC Syst Biol. 2008 Dec 19;2:109. doi: 10.1186/1752-0509-2-109.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.