Gene - combatTB X

papA5

Type: protein_coding
Name: papA5
Locus Name: Rv2939
Product: Possible conserved polyketide synthase associated protein PapA5
Functional Category: Lipid metabolism
Location: 3274949..3276217 (+ strand)
Gene Length: 1268 bp
Nucleotides: TGTTTCCCGGATCTGTGATCCGAAAGCTGTCGCACAGCGAGGAAGTCTTCGCGCAGTACGAGGTTTTTACTTCCATGACAATCCAGCTGCGCGGTGTTATCGATGTCGATGCGCTGTCGGATGCCTTCGACGCCCTCTTGGAAACCCACCCAGTCCTGGCCAGCCACCTTGAGCAAAGCTCCGACGGCGGTTGGAATCTCGTTGCCGACGACCTGCTGCACTCTGGAATCTGTGTCATCGACGGCACGGCCGCCACCAACGGGTCACCGTCGGGAAACGCCGAACTACGGCTCGACCAGAGCGTGTCCCTATTGCATCTGCAGCTGATCCTCCGCGAAGGAGGAGCCGAGCTGACGCTATACCTCCATCACTGCATGGCCGATGGTCATCACGGGGCCGTTCTCGTCGACGAGCTGTTCTCCCGCTACACCGACGCGGTCACTACCGGTGACCCCGGCCCGATAACCCCGCAGCCCACGCCGCTGTCAATGGAGGCTGTGCTGGCACAGCGGGGTATCAGGAAGCAAGGGCTTTCGGGAGCTGAACGTTTTATGTCGGTGATGTATGCCTATGAGATCCCTGCCACCGAGACGCCGGCGGTCCTCGCGCATCCTGGGCTGCCCCAAGCTGTTCCGGTCACCCGACTCTGGCTTTCCAAGCAGCAGACATCGGACCTCATGGCGTTCGGCCGCGAGCATCGCCTCAGCCTTAACGCCGTGGTCGCGGCAGCCATCCTGCTGACCGAGTGGCAGCTGCGCAACACCCCGCACGTCCCGATTCCCTACGTTTACCCCGTCGACCTGCGATTTGTTCTAGCTCCCCCAGTGGCCCCGACAGAAGCTACCAATCTCCTCGGGGCGGCGTCTTACCTCGCTGAGATCGGGCCGAATACCGACATCGTGGATCTGGCAAGCGATATCGTTGCCACACTTCGGGCTGACTTGGCCAATGGTGTGATTCAGCAGTCGGGGCTCCACTTCGGCACGGCATTCGAAGGAACTCCTCCCGGCCTACCACCACTTGTCTTCTGCACTGACGCCACTTCATTTCCCACCATGCGCACACCGCCGGGCCTGGAGATCGAAGACATTAAGGGCCAATTCTATTGTTCGATCAGCGTCCCCCTCGATCTGTACTCGTGTGCCGTTTACGCAGGACAACTGATCATCGAGCATCATGGGCACATCGCGGAACCGGGGAAGTCCCTCGAGGCGATACGTTCACTGCTGTGCACCGTTCCCTCGGAGTATGGCTGGATCATGGAGTGA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Catalyzes diesterification of phthiocerol, phthiodiolone, and phenolphthiocerol with mycocerosic acids, the final step in the phthiocerol, phthiodiolone and phenolphthiocerol dimycocerosate esters (PDIM) synthesis. Can directly transfer the mycocerosate bound to the mycocerosic acid synthase (mas) onto the substrate alcohols. Is also able to catalyze acyl transfer using various nucleophiles as acceptors and several acyl-CoA thioesters as donors in vitro; preference is observed for saturated medium chain alcohols and long chain acyl-CoA thioesters. {ECO:0000269|PubMed:15070765, ECO:0000269|PubMed:15749014, ECO:0000269|PubMed:22361940}.

Family

Acyltransferase PapA5 family

GO

DIM/DIP cell wall layer assembly GO:0071770 biological_process
lipid biosynthetic process GO:0008610 biological_process
O-acyltransferase activity GO:0008374 molecular_function
transferase activity, transferring acyl groups other than amino-acyl groups GO:0016747 molecular_function

InterPro

UniProt: P9WIN5
GenBank: Rv2939
EnsemblBacteria: Rv2939
Mycobrowser: Rv2939

Summary

Name: Phthiocerol/phthiodiolone dimycocerosyl transferase (EC 2.3.1.282) (Acyltransferase PapA5) (Phthiocerol/phthiodiolone O-acyltransferase) (Polyketide synthase-associated protein A5)
Family: Acyltransferase PapA5 family
Protein Sequence: MFPGSVIRKLSHSEEVFAQYEVFTSMTIQLRGVIDVDALSDAFDALLETHPVLASHLEQSSDGGWNLVADDLLHSGICVIDGTAATNGSPSGNAELRLDQSVSLLHLQLILREGGAELTLYLHHCMADGHHGAVLVDELFSRYTDAVTTGDPGPITPQPTPLSMEAVLAQRGIRKQGLSGAERFMSVMYAYEIPATETPAVLAHPGLPQAVPVTRLWLSKQQTSDLMAFGREHRLSLNAVVAAAILLTEWQLRNTPHVPIPYVYPVDLRFVLAPPVAPTEATNLLGAASYLAEIGPNTDIVDLASDIVATLRADLANGVIQQSGLHFGTAFEGTPPGLPPLVFCTDATSFPTMRTPPGLEIEDIKGQFYCSISVPLDLYSCAVYAGQLIIEHHGHIAEPGKSLEAIRSLLCTVPSEYGWIME
Mass: 45,429 Da
Length: 422 Aa

Rv2939 doesn't seem to be a targeted by any drug.

R-MTU-9635470 R-MTU-9635470
R-MTU-9635470
R-MTU-9635470

Crystal structure of PapA5, a phthiocerol dimycocerosyl transferase from Mycobacterium tuberculosis.: J Biol Chem. 2004 Jul 16;279(29):30634-42. doi: 10.1074/jbc.M404011200. Epub 2004 May 3.
Diacyltransferase Activity and Chain Length Specificity of Mycobacterium tuberculosis PapA5 in the Synthesis of Alkyl beta-Diol Lipids.: Biochemistry. 2015 Sep 8;54(35):5457-68. doi: 10.1021/acs.biochem.5b00455. Epub 2015 Aug 24.
The mycobacterial acyltransferase PapA5 is required for biosynthesis of cell wall-associated phenolic glycolipids.: Microbiology. 2012 May;158(Pt 5):1379-87. doi: 10.1099/mic.0.057869-0. Epub 2012 Feb 23.
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Forkhead-associated domain-containing protein Rv0019c and polyketide-associated protein PapA5, from substrates of serine/threonine protein kinase PknB to interacting proteins of Mycobacterium tuberculosis.: J Biol Chem. 2009 Dec 11;284(50):34723-34. doi: 10.1074/jbc.M109.058834. Epub 2009 Oct 13.
targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis.: BMC Syst Biol. 2008 Dec 19;2:109. doi: 10.1186/1752-0509-2-109.
Dissecting the mechanism and assembly of a complex virulence mycobacterial lipid.: Mol Cell. 2005 Mar 4;17(5):631-43. doi: 10.1016/j.molcel.2005.02.009.
Mycobacterial polyketide-associated proteins are acyltransferases: proof of principle with Mycobacterium tuberculosis PapA5.: Proc Natl Acad Sci U S A. 2004 Mar 30;101(13):4608-13. doi: 10.1073/pnas.0306928101. Epub 2004 Mar 18.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.