nrdE
- Type
- protein_coding
- Name
- nrdE
- Locus Name
-
Rv3051c
- Product
-
Ribonucleoside-diphosphate reductase (alpha chain) NrdE (ribonucleotide reductase small subunit) (R1F protein)
- Functional Category
-
Information pathways
- Location
- 3412085..3414166 (- strand)
- Gene Length
- 2081 bp
- Nucleotides
-
ATGCTGAACCTGTACGACGCGGACGGCAAGATCCAGTTCGACAAGGATCGGGAAGCAGCCCACCAGTACTTTTTGCAGCATGTCAATCAGAACACGGTCTTCTTCCATAATCAGGACGAGAAGCTCGACTACCTGATCCGCGAGAATTACTACGAGCGTGAGGTTCTCGACCAGTACTCGCGCAACTTCGTCAAGACGCTGCTAGACCGCGCCTACGCCAAAAAGTTCCGGTTTCCGACGTTTTTGGGTGCGTTCAAGTACTACACCTCCTACACGCTGAAAACCTTTGACGGGAAGCGCTATCTGGAGCGCTTCGAGGACCGCGTGGTCATGGTGGCGCTAACGTTGGCCGCCGGCGATACCGCACTTGCCGAGCTGCTGGTCGACGAGATCATCGACGGCCGCTTCCAGCCCGCCACACCGACGTTTTTGAATTCTGGCAAGAAGCAGCGCGGGGAGCCCGTGAGCTGTTTTTTGCTTCGCGTCGAAGACAACATGGAGTCGATCGGACGGTCGATCAACTCCGCGCTGCAGCTATCCAAGCGTGGCGGGGGAGTGGCGTTGCTGCTGACCAACATTCGCGAGCACGGCGCGCCGATCAAGAACATCGAGAACCAGTCCTCGGGCGTCATCCCCATCATGAAGTTGCTGGAGGATGCGTTCTCCTACGCCAACCAGCTGGGCGCTCGTCAAGGTGCCGGCGCGGTGTACCTGCACGCCCATCACCCCGACATCTACCGATTCCTGGACACCAAGCGTGAGAACGCCGACGAGAAGATCCGGATCAAGACGCTGAGTCTGGGGGTGGTGATCCCCGACATCACCTTCGAGTTGGCCAAGCGCAACGATGACATGTACCTGTTCTCGCCCTACGATGTCGAGCGGGTCTACGGTGTGCCGTTCGCTGACATCTCGGTCACCGAGAAGTACTACGAAATGGTCGATGACGCGCGCATCCGCAAGACCAAGATCAAGGCACGGGAGTTCTTCCAGACGCTGGCCGAGCTGCAGTTCGAGTCCGGCTACCCCTATATCATGTTCGAAGACACCGTCAATCGCGCTAATCCAATTGATGGCAAGATCACGCACAGCAACCTGTGCTCGGAGATCCTGCAAGTGTCTACGCCGTCATTGTTCAACGAGGACTTGTCGTATGCCAAAGTGGGCAAAGACATTTCGTGCAACCTGGGGTCGCTGAACATCGCCAAGACGATGGACTCGCCGGACTTCGCGCAGACGATCGAGGTGGCGATCCGCGCGTTGACCGCGGTGAGCGACCAAACCCATATCAAGTCGGTGCCCTCAATCGAGCAGGGCAACAACGACTCCCACGCGATCGGGCTAGGACAGATGAACCTGCACGGCTACCTGGCCCGGGAACGCATCTTCTACGGATCCGACGAAGGCATCGACTTCACCAACATCTACTTCTATACGGTGCTGTATCACGCGTTGCGGGCATCCAACCGCATCGCGATCGAACGCGGCACGCACTTCAAGGGTTTCGAGCGGTCCAAGTACGCGTCCGGGGAATTCTTCGACAAGTACACCGACCAGATTTGGGAGCCGAAGACCCAGAAGGTACGCCAGCTGTTCGCCGACGCCGGCATCCGCATCCCAACGCAGGACGACTGGCGTCGGCTCAAGGAGTCGGTGCAAGCGCACGGCATCTACAACCAGAACCTGCAGGCGGTGCCGCCGACCGGGTCGATTTCCTACATCAACCATTCGACGTCGTCGATTCACCCGATCGTGTCGAAGGTCGAGATCCGCAAGGAAGGCAAGATCGGGCGGGTCTACTACCCGGCGCCGTATATGACCAACGACAACCTGGAGTACTACGAAGACGCCTACGAGATCGGTTACGAGAAGATCATCGACACCTACGCGGCGGCCACCCAGCATGTGGATCAAGGGCTTTCGCTGACGTTGTTCTTCAAAGACACCGCCACCACCCGCGACGTGAACAAGGCGCAGATTTACGCCTGGCGCAAGGGGATCAAGACGCTGTACTACATCCGGCTGCGGCAGATGGCGTTGGAGGGCACCGAGGTCGAGGGTTGCGTGTCCTGCATGCTGTA
- Drug Resistance
- Mutations
- Function
- Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. When coexpressed in E.coli with nrdF2 the 2 proteins complement a temperature-sensitive E.coli mutant, however coexpression with nrdF1 does not complement. {ECO:0000269|PubMed:9335290}.
- Family
-
Ribonucleoside diphosphate reductase large chain family
- GO
-
- deoxyribonucleotide biosynthetic process GO:0009263 biological_process
- ribonucleoside-diphosphate reductase complex GO:0005971 cellular_component
- ATP binding GO:0005524 molecular_function
- CDP reductase activity GO:0051063 molecular_function
- ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor GO:0004748 molecular_function
- oxidation-reduction process GO:0055114 biological_process
- plasma membrane GO:0005886 cellular_component
- DNA replication GO:0006260 biological_process
- InterPro
Summary
- Name
- Ribonucleoside-diphosphate reductase subunit alpha (EC 1.17.4.1) (Ribonucleotide reductase R1 subunit)
- Family
- Ribonucleoside diphosphate reductase large chain family
- Protein Sequence
-
MPPTVIAEPVASGAHASYSGGPGETDYHALNAMLNLYDADGKIQFDKDREAAHQYFLQHVNQNTVFFHNQDEKLDYLIRENYYEREVLDQYSRNFVKTLLDRAYAKKFRFPTFLGAFKYYTSYTLKTFDGKRYLERFEDRVVMVALTLAAGDTALAELLVDEIIDGRFQPATPTFLNSGKKQRGEPVSCFLLRVEDNMESIGRSINSALQLSKRGGGVALLLTNIREHGAPIKNIENQSSGVIPIMKLLEDAFSYANQLGARQGAGAVYLHAHHPDIYRFLDTKRENADEKIRIKTLSLGVVIPDITFELAKRNDDMYLFSPYDVERVYGVPFADISVTEKYYEMVDDARIRKTKIKAREFFQTLAELQFESGYPYIMFEDTVNRANPIDGKITHSNLCSEILQVSTPSLFNEDLSYAKVGKDISCNLGSLNIAKTMDSPDFAQTIEVAIRALTAVSDQTHIKSVPSIEQGNNDSHAIGLGQMNLHGYLARERIFYGSDEGIDFTNIYFYTVLYHALRASNRIAIERGTHFKGFERSKYASGEFFDKYTDQIWEPKTQKVRQLFADAGIRIPTQDDWRRLKESVQAHGIYNQNLQAVPPTGSISYINHSTSSIHPIVSKVEIRKEGKIGRVYYPAPYMTNDNLEYYEDAYEIGYEKIIDTYAAATQHVDQGLSLTLFFKDTATTRDVNKAQIYAWRKGIKTLYYIRLRQMALEGTEVEGCVSCML
- Mass
- 82,441 Da
- Length
- 725 Aa
Rv3051c doesn't seem to be a targeted by any drug.
-
-
Purine metabolism mtu00230
mtu00230PATHWAY_MAP mtu00230 Purine metabolism
-
- Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.
- Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
- Ribonucleotide reduction in Mycobacterium tuberculosis: function and expression of genes encoding class Ib and class II ribonucleotide reductases.
- Infect Immun. 2003 Nov;71(11):6124-31. doi: 10.1128/iai.71.11.6124-6131.2003.
- Characterization of two genes encoding the Mycobacterium tuberculosis ribonucleotide reductase small subunit.
- J Bacteriol. 1997 Oct;179(20):6408-15. doi: 10.1128/jb.179.20.6408-6415.1997.
- Isolation of ribonucleotide reductase from Mycobacterium tuberculosis and cloning, expression, and purification of the large subunit.
- J Bacteriol. 1994 Nov;176(21):6738-43. doi: 10.1128/jb.176.21.6738-6743.1994.
- Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.
- Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.