Gene - combatTB X

ligB

Type: protein_coding
Name: ligB
Locus Name: Rv3062
Product: Probable ATP-dependent DNA ligase LigB (polydeoxyribonucleotide synthase [ATP]) (polynucleotide ligase [ATP]) (sealase) (DNA repair protein) (DNA joinase)
Functional Category: Information pathways
Location: 3425584..3427107 (+ strand)
Gene Length: 1523 bp
Nucleotides: TGCTCCTTCACGACGTGGCCATCACCTCAATGGACGTGGCCGCCACCTCGTCGCGGCTGACCAAGGTCGCGCGCATCGCCGCCCTGTTGCACCGCGCCGCGCCAGACACACAGCTGGTCACGATCATCGTGTCGTGGCTCTCCGGCGAGCTGCCGCAACGCCATATCGGTGTCGGGTGGGCGGCATTGCGGTCCCTACCGCCGCCCGCGCCGCAACCGGCGTTGACCGTCACCGGTGTCGACGCCACCCTCTCTAAGATCGGCACTCTACCGGGCAAAGGGTCTCAGGCGCAGCGCGCGGCACTCGTTGCGGAATTGTTCTCCGCCGCAACCGAAGCTGAGCAAACCTTTTTGTTGCGACTGCTCGGCGGTGAACTGCGCCAGGGCGCAAAGGGCGGGATCATGGCCGATGCGGTCGCCCAGGCCGCCGGGCTCCCGGCCGCGACGGTCCAACGCGCCGCGATGCTAGGCGGCGACCTGGCGGCAGCGGCGGCGGCCGGCCTGTCCGGCGCGGCGCTGGACACCTTCACCCTGCGAGTGGGCCGACCGATAGGCCCGATGCTGGCACAGACCGCGACCAGCGTCCATGATGCACTCGAACGTCACGGCGGCACAACCATTTTCGAGGCTAAACTAGACGGCGCGCGAGTGCAGATCCACCGGGCAAACGACCAGGTCAGGATCTACACCCGAAGCCTGGACGACGTCACTGCCCGGCTGCCCGAGGTGGTGGAGGCAACACTGGCACTGCCGGTCCGGGATCTAGTGGCCGACGGCGAGGCGATCGCGCTGTGCCCGGACAACCGGCCGCAGCGTTTCCAGGTCACCGCATCACGGTTCGGCCGATCGGTCGATGTTGCGGCTGCCCGCGCGACGCAGCCACTTTCGGTGTTCTTCTTCGACATCCTGCATCGGGATGGTACCGACTTGCTCGAAGCGCCGACCACCGAGCGGCTGGCCGCCCTGGACGCACTGGTGCCGGCTCGGCACCGCGTGGACCGGCTGATCACGTCCGATCCAACGGACGCGGCCAACTTCCTGGATGCGACGCTGGCCGCCGGCCACGAGGGGGTGATGGCCAAGGCACCGGCCGCTCGTTACCTTGCGGGTCGCCGCGGAGCGGGCTGGCTGAAGGTCAAGCCGGTGCACACACTCGACTTGGTGGTGCTCGCGGTGGAATGGGGCTCGGGACGCCGGCGCGGCAAGCTCTCCAATATTCACCTGGGCGCACGCGATCCGGCTACCGGTGGATTCGTGATGGTGGGCAAGACCTTCAAAGGAATGACCGACGCCATGCTGGACTGGCAGACCACCAGGTTTCACGAGATCGCGGTGGGTCCGACAGACGGCTACGTCGTCCAACTTAGGCCCGAGCAGGTGGTCGAGGTAGCCCTCGACGGCGTGCAAAGGTCGTCGCGCTACCCGGGCGGGCTGGCATTGCGGTTTGCCCGCGTGGTGCGCTACCGCGCCGACAAGGACCCGGCCGAGGCCGACACCATCGATGCCGTGCGCGCGCTCTACTGA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. {ECO:0000255|HAMAP-Rule:MF_00407, ECO:0000269|PubMed:14985346}.

Family

ATP-dependent DNA ligase family

GO

DNA biosynthetic process GO:0071897 biological_process
cell cycle GO:0007049 biological_process
magnesium ion binding GO:0000287 molecular_function
DNA ligase (ATP) activity GO:0003910 molecular_function
cell wall GO:0005618 cellular_component
DNA ligation involved in DNA repair GO:0051103 biological_process
cell division GO:0051301 biological_process
cytoplasm GO:0005737 cellular_component
DNA recombination GO:0006310 biological_process
DNA ligation GO:0006266 biological_process
lagging strand elongation GO:0006273 biological_process
DNA binding GO:0003677 molecular_function
ATP binding GO:0005524 molecular_function

InterPro

UniProt: P9WNV5
GenBank: Rv3062
EnsemblBacteria: Rv3062
Mycobrowser: Rv3062

Summary

Name: DNA ligase B (LigB) (EC 6.5.1.1) (Polydeoxyribonucleotide synthase [ATP])
Family: ATP-dependent DNA ligase family
Protein Sequence: MLLHDVAITSMDVAATSSRLTKVARIAALLHRAAPDTQLVTIIVSWLSGELPQRHIGVGWAALRSLPPPAPQPALTVTGVDATLSKIGTLPGKGSQAQRAALVAELFSAATEAEQTFLLRLLGGELRQGAKGGIMADAVAQAAGLPAATVQRAAMLGGDLAAAAAAGLSGAALDTFTLRVGRPIGPMLAQTATSVHDALERHGGTTIFEAKLDGARVQIHRANDQVRIYTRSLDDVTARLPEVVEATLALPVRDLVADGEAIALCPDNRPQRFQVTASRFGRSVDVAAARATQPLSVFFFDILHRDGTDLLEAPTTERLAALDALVPARHRVDRLITSDPTDAANFLDATLAAGHEGVMAKAPAARYLAGRRGAGWLKVKPVHTLDLVVLAVEWGSGRRRGKLSNIHLGARDPATGGFVMVGKTFKGMTDAMLDWQTTRFHEIAVGPTDGYVVQLRPEQVVEVALDGVQRSSRYPGGLALRFARVVRYRADKDPAEADTIDAVRALY
Mass: 53,737 Da
Length: 507 Aa

Rv3062 doesn't seem to be a targeted by any drug.

Mismatch repair mtu03430
mtu03430
The Escherichia coli MMR pathway has been extensively studied and is well characterized. In E. coli, the mismatch-activated MutS-MutL-ATP complex licenses MutH to incise the nearest unmethylated GATC sequence. UvrD and an exonuclease generate a gap. This gap is filled by pol III and DNA ligase. The GATC sites are then methylated by Dam. Several human MMR proteins have been identified based on their homology to E. coli MMR proteins. These include human homologs of MutS and MutL. Although E. coli MutS and MutL proteins are homodimers, human MutS and MutL homologs are heterodimers. The role of hemimethylated dGATC sites as a signal for strand discrimination is not conserved from E. coli to human. Human MMR is presumed to be nick-directed in vivo, and is thought to discriminate daughter and template strands using a strand-specific nick.

Nucleotide excision repair mtu03420
mtu03420
Genetic Information Processing; Replication and repair

Base excision repair mtu03410
mtu03410
Genetic Information Processing; Replication and repair

DNA replication mtu03030
mtu03030
Genetic Information Processing; Replication and repair

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Biochemical and genetic analysis of the four DNA ligases of mycobacteria.: J Biol Chem. 2004 May 14;279(20):20594-606. doi: 10.1074/jbc.M401841200. Epub 2004 Feb 25.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.