pknK
- Type
- protein_coding
- Name
- pknK
- Locus Name
-
Rv3080c
- Product
-
Serine/threonine-protein kinase transcriptional regulatory protein PknK (protein kinase K) (STPK K)
- Functional Category
-
Regulatory proteins
- Location
- 3442656..3445988 (- strand)
- Gene Length
- 3332 bp
- Nucleotides
-
ATGACCGACGTTGATCCGCACGCGACGCGGCGGGACCTGGTCCCGAATATTCCCGCGGAACTGCTTGAGGCTGGATTCGACAATGTCGAGGAGATCGGGCGCGGCGGATTCGGCGTCGTCTACCGCTGCGTCCAGCCCTCGCTGGACCGCGCCGTCGCCGTCAAGGTATTGAGCACCGACCTGGATCGGGACAATCTCGAGCGCTTCCTGCGCGAGCAGCGGGCCATGGGCCGCCTTTCCGGGCACCCGCACATCGTGACCGTCTTGCAGGTGGGCGTGTTGGCGGGTGGGCGGCCCTTCATCGTGATGCCCTACCACGCCAAGAATTCGTTGGAGACGCTGATTCGCCGGCACGGGCCGCTGGACTGGCGCGAGACGCTGTCGATCGGCGTCAAGCTCGCGGGAGCGCTGGAAGCCGCGCATCGCGTCGGCACCCTGCACCGTGACGTGAAGCCGGGGAATATCCTGCTGACCGACTACGGGGAACCGCAGCTGACCGATTTCGGAATCGCCAGAATCGCCGGGGGTTTCGAGACGGCGACCGGGGTGATTGCCGGTTCCCCGGCTTTCACCGCGCCGGAAGTTCTCGAAGGAGCATCGCCGACGCCCGCCTCTGACGTGTACTCCCTGGGCGCGACGTTGTTCTGTGCGCTGACCGGCCATGCCGCCTACGAGCGCCGCAGCGGTGAGCGGGTGATCGCCCAGTTCCTGCGGATCACCTCGCAGCCGATCCCCGACCTGCGGAAGCAGGGACTGCCCGCGGACGTGGCCGCCGCCATCGAACGGGCGATGGCCCGCCATCCGGCGGATCGTCCCGCGACCGCGGCAGACGTTGGCGAGGAGCTTCGCGACGTTCAGCGCCGCAACGGCGTCAGCGTCGACGAGATGCCCCTCCCCGTCGAGCTGGGCGTGGAACGCCGACGCTCGCCCGAGGCGCACGCGGCGCATCGGCATACCGGCGGCGGCACCCCGACGGTCCCGACGCCTCCGACACCCGCGACCAAGTACCGGCCGTCGGTGCCCACCGGCTCGCTGGTCACCCGCAGCCGGCTCACCGACATCCTGCGCGCCGGCGGACGGCGCCGGCTGATCCTCATCCACGCGCCCTCGGGATTCGGCAAAAGCACCCTGGCGGCGCAATGGCGGGAAGAGCTCTCGCGCGACGGCGCCGCGGTCGCCTGGCTGACAATCGACAACGACGACAACAACGAGGTGTGGTTCTTGTCGCACCTGCTCGAGTCGATCCGGCGGGTCCGGCCCACGCTGGCCGAGTCGTTGGGGCACGTGCTCGAAGAGCATGGGGATGACGCCGGCCGCTACGTGTTGACTTCGCTGATCGACGAAATCCACGAAAACGACGACCGGATCGCGGTGGTGATCGACGACTGGCATCGGGTGTCCGACAGCCGCACCCAAGCTGCCCTGGGTTTCCTGCTGGACAACGGATGTCACCACCTGCAGCTCATCGTGACCAGCTGGTCTCGCGCCGGTTTGCCGGTGGGCAGGTTGCGGATCGGCGACGAACTAGCCGAGATCGATTCGGCTGCTTTGCGCTTCGATACCGACGAGGCCGCCGCGCTGCTGAACGATGCTGGTGGTCTGCGATTGCCGCGCGCAGACGTGCAGGCGCTGACTACCTCTACCGACGGGTGGGCCGCGGCGCTGCGGCTGGCCGCGCTGTCGCTGCGCGGCGGGGGCGACGCGACCCAACTCCTGCGCGGACTTTCCGGCGCCAGTGACGTGATCCACGAATTCCTGAGCGAAAACGTGCTGGACACCCTGGAACCCGAACTGCGCGAATTCCTACTGGTGGCATCGGTCACCGAACGCACGTGCGGCGGGCTGGCCTCGGCGCTGGCCGGGATCACCAATGGGCGGGCGATGCTGGAAGAGGCCGAGCACCGCGGCTTGTTCCTGCAACGGACCGAAGACGACCCGAATTGGTTTCGCTTCCACCAAATGTTCGCCGACTTTCTCCACCGTCGCCTCGAACGTGGCGGGTCGCACCGGGTGGCGGAACTGCACCGCAGGGCATCGGCCTGGTTCGCCGAGAACGGCTACCTGCACGAAGCCGTCGACCATGCACTGGCCGCGGGCGATCCCGCGCGCGCCGTCGATCTTGTCGAGCAGGATGAAACGAACCTGCCGGAGCAGTCAAAGATGACCACACTTCTGGCAATCGTGCAGAAACTGCCGACGTCGATGGTGGTTTCACGGGCCCGGCTCCAACTCGCCATCGCGTGGGCGAACATTCTGCTGCAACGGCCGGCGCCGGCCACCGGTGCCCTGAATCGTTTCGAAACGGCCCTTGGCCGGGCCGAGCTTCCCGAGGCGACGCAGGCGGATCTGCGGGCCGAGGCAGACGTGTTGCGGGCGGTCGCCGAGGTGTTCGCAGACCGGGTCGAGCGCGTGGATGACCTTCTCGCCGAGGCAATGTCGAGACCGGACACCCTGCCCCCGCGAGTCCCCGGGACCGCCGGCAACACCGCGGCGTTGGCCGCGATCTGCCGCTTCGAGTTCGCCGAGGTATATCCACTGCTGGACTGGGCCGCGCCCTACCAGGAAATGATGGGACCGTTCGGCACCGTTTATGCGCAGTGCTTGCGCGGCATGGCGGCCAGGAATCGGCTCGACATTGTCGCTGCGCTACAGAACTTCCGAACGGCGTTCGAGGTCGGCACGGCAGTGGGGGCCCACTCGCACGCGGCGCGGCTTGCGGGTTCGCTGCTCGCCGAATTGCTCTACGAGACCGGCGATCTGGCCGGGGCTGGTCGTCTCATGGACGAGAGCTATCTGCTGGGTTCCGAGGGGGGTGCAGTGGACTACCTGGCCGCCAGGTACGTGATCGGCGCGCGGGTCAAGGCGGCCCAGGGGGATCATGAGGGTGCGGCTGATCGCCTGTCCACCGGAGGCGATACTGCCGTCCAGCTGGGGCTGCCGCGCCTGGCTGCCCGAATCAACAACGAGCGGATCCGGCTGGGCATCGCGCTACCTGCGGCGGTGGCCGCCGATTTGCTGGCACCCCGCACCATCCCCCGCGACAATGGAATCGCCACCATGACAGCCGAACTCGACGAGGACTCCGCGGTGCGCCTGTTGTCCGCCGGCGACTCCGCCGATCGTGACCAAGCCTGCCAACGGGCCGGTGCTCTCGCCGCCGCCATCGACGGTACGCGCAGACCGCTGGCGGCGCTGCAGGCGCAAATACTTCATATCGAAACGCTTGCCGCCACCGGACGGGAATCCGATGCGCGAAACGAACTGGCGCCGGTAGCCACGAAGTGCGCCGAACTCGGGCTGTCACGTCTGCTGGTCGATGCGGGACTGGCCTA
- Drug Resistance
- Mutations
- Function
- Key microbial factor involved in regulation of early and late events in tuberculosis infection, and in host-pathogen interactions. Modulates host immunity during early infection. Slows mycobacterial growth during chronic infection in host and during a variety of stress conditions in vitro. Regulates the expression of a large subset of tRNA genes as a means to facilitate adaptation to changing growth environments. In vitro, directs the inhibition of transcription and translation processes in a phosphorylation-dependent manner. Phosphorylates the transcriptional regulator VirS, thereby increasing the affinity of VirS for the mycobacterial monooxygenase (mymA) promoter. In vitro, can also phosphorylate the mycobacterial monooxygenase operon products Rv3083 (MymA), Rv3084 (LipR), Rv3085 and Rv3088. {ECO:0000269|PubMed:19251699, ECO:0000269|PubMed:20522497, ECO:0000269|PubMed:22661693, ECO:0000269|PubMed:22740025}.
- Family
-
Protein kinase superfamily, Ser/Thr protein kinase family
- GO
-
- protein kinase activity GO:0004672 molecular_function
- protein serine/threonine kinase activity GO:0004674 molecular_function
- regulation of DNA-binding transcription factor activity GO:0051090 biological_process
- plasma membrane GO:0005886 cellular_component
- identical protein binding GO:0042802 molecular_function
- cytosol GO:0005829 cellular_component
- ATP binding GO:0005524 molecular_function
- extracellular region GO:0005576 cellular_component
- cell wall GO:0005618 cellular_component
- pathogenesis GO:0009405 biological_process
- protein autophosphorylation GO:0046777 biological_process
- metal ion binding GO:0046872 molecular_function
- InterPro
Summary
- Name
- Serine/threonine-protein kinase PknK (EC 2.7.11.1) (Protein kinase K)
- Family
- Protein kinase superfamily, Ser/Thr protein kinase family
- Protein Sequence
-
MTDVDPHATRRDLVPNIPAELLEAGFDNVEEIGRGGFGVVYRCVQPSLDRAVAVKVLSTDLDRDNLERFLREQRAMGRLSGHPHIVTVLQVGVLAGGRPFIVMPYHAKNSLETLIRRHGPLDWRETLSIGVKLAGALEAAHRVGTLHRDVKPGNILLTDYGEPQLTDFGIARIAGGFETATGVIAGSPAFTAPEVLEGASPTPASDVYSLGATLFCALTGHAAYERRSGERVIAQFLRITSQPIPDLRKQGLPADVAAAIERAMARHPADRPATAADVGEELRDVQRRNGVSVDEMPLPVELGVERRRSPEAHAAHRHTGGGTPTVPTPPTPATKYRPSVPTGSLVTRSRLTDILRAGGRRRLILIHAPSGFGKSTLAAQWREELSRDGAAVAWLTIDNDDNNEVWFLSHLLESIRRVRPTLAESLGHVLEEHGDDAGRYVLTSLIDEIHENDDRIAVVIDDWHRVSDSRTQAALGFLLDNGCHHLQLIVTSWSRAGLPVGRLRIGDELAEIDSAALRFDTDEAAALLNDAGGLRLPRADVQALTTSTDGWAAALRLAALSLRGGGDATQLLRGLSGASDVIHEFLSENVLDTLEPELREFLLVASVTERTCGGLASALAGITNGRAMLEEAEHRGLFLQRTEDDPNWFRFHQMFADFLHRRLERGGSHRVAELHRRASAWFAENGYLHEAVDHALAAGDPARAVDLVEQDETNLPEQSKMTTLLAIVQKLPTSMVVSRARLQLAIAWANILLQRPAPATGALNRFETALGRAELPEATQADLRAEADVLRAVAEVFADRVERVDDLLAEAMSRPDTLPPRVPGTAGNTAALAAICRFEFAEVYPLLDWAAPYQEMMGPFGTVYAQCLRGMAARNRLDIVAALQNFRTAFEVGTAVGAHSHAARLAGSLLAELLYETGDLAGAGRLMDESYLLGSEGGAVDYLAARYVIGARVKAAQGDHEGAADRLSTGGDTAVQLGLPRLAARINNERIRLGIALPAAVAADLLAPRTIPRDNGIATMTAELDEDSAVRLLSAGDSADRDQACQRAGALAAAIDGTRRPLAALQAQILHIETLAATGRESDARNELAPVATKCAELGLSRLLVDAGLA
- Mass
- 119,418 Da
- Length
- 1110 Aa
Rv3080c doesn't seem to be a targeted by any drug.
Rv3080c doesn't seem to be involved in any pathway.
- Functional characterization delineates that a Mycobacterium tuberculosis specific protein kinase (Rv3080c) is responsible for the growth, phagocytosis and intracellular survival of avirulent mycobacteria.
- Mol Cell Biochem. 2012 Oct;369(1-2):67-74. doi: 10.1007/s11010-012-1369-9. Epub 2012 Jun 28.
- Mycobacterium tuberculosis protein kinase K enables growth adaptation through translation control.
- J Bacteriol. 2012 Aug;194(16):4184-96. doi: 10.1128/JB.00585-12. Epub 2012 Jun 1.
- Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.
- Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.
- The Mycobacterium tuberculosis protein kinase K modulates activation of transcription from the promoter of mycobacterial monooxygenase operon through phosphorylation of the transcriptional regulator VirS.
- J Biol Chem. 2009 Apr 24;284(17):11090-9. doi: 10.1074/jbc.M808705200. Epub 2009 Feb 27.
- Mycobacterium tuberculosis protein kinase K confers survival advantage during early infection in mice and regulates growth in culture and during persistent infection: implications for immune modulation.
- Microbiology. 2010 Sep;156(Pt 9):2829-41. doi: 10.1099/mic.0.040675-0. Epub 2010 Jun 3.
- Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.
- Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.