Gene - combatTB X

desA3

Type: protein_coding
Name: desA3
Locus Name: Rv3229c
Product: Possible linoleoyl-CoA desaturase (delta(6)-desaturase)
Functional Category: Lipid metabolism
Location: 3605751..3607034 (- strand)
Gene Length: 1283 bp
Nucleotides: ATGGCGATCACTGACGTCGACGTATTCGCGCATCTGACGGACGCCGACATTGAAAACCTGGCCGCCGAGCTAGATGCCATCCGCCGAGACGTTGAAGAATCGCGCGGCGAACGCGATGCCCGCTACATCCGCCGCACCATCGCCGCACAGCGGGCGCTCGAGGTGTCCGGTCGGCTACTGCTGGCTGGCAGCTCACGACGCCTCGCCTGGTGGACAGGTGCCCTGACCCTTGGCGTGGCCAAGATCATCGAAAACATGGAGATCGGCCACAACGTCATGCACGGCCAGTGGGACTGGATGAACGACCCGGAGATTCACTCCTCGACGTGGGAGTGGGACATGAGCGGATCGTCCAAGCACTGGCGCTACACCCACAACTTCGTGCACCACAAATACACCAACATCCTCGGTATGGATGACGACGTCGGATACGGCATGCTGCGCGTCACCCGTGACCAGCGCTGGAAGCGCTACAACATCTTCAACGTGGTGTGGAACACCATCCTTGCGATCGGCTTCGAGTGGGGAGTTGCGCTGCAGCACTTGGAGATCGGCAAGATCTTCAAGGGTCGGGCCGACCGCGAGGCCGCTAAAACCCGGTTGCGTGAATTCTCAGCCAAAGCGGGCCGCCAGGTGTTCAAGGACTATGTCGCGTTCCCGGCGCTGACCTCGTTGTCCCCGGGGGCGACGTATCGGTCCACTTTGACCGCCAACGTGGTGGCCAACGTGATCCGCAACGTGTGGTCCAACGCGGTGATCTTCTGCGGCCATTTCCCGGATGGCGCAGAGAAATTCACCAAGACGGACATGATCGGTGAGCCGAAGGGCCAGTGGTACCTACGGCAGATGCTGGGCAGCGCAAACTTCAACGCTGGGCCGGCGCTGCGGTTCATGAGCGGCAATCTGTGCCACCAAATCGAGCATCACCTCTATCCGGACCTGCCGAGCAACCGGCTTCACGAGATTTCGGTGCGGGTGCGTGAGGTCTGCGACAGATACGACTTGCCATACACCACGGGCTCGTTCTTGGTGCAGTACGGCAAGACCTGGCGCACCCTTGCCAAGCTGTCGTTGCCGGATAAGTACTTACGTGACAACGCTGACGATGCACCCGAGACCCGCAGTGAGCGGATGTTCGCTGGTCTGGGGCCGGGTTTCGCGGGCGCCGATCCGGTGACCGGACGTCGTCGGGGGTTAAAGACCGCGATTGCCGCCGTGCGGGGTCGGCGGCGCAGCAAGCGTATGGCTAAGTCGGTCACCGAACCCGACGATCTGGCAGCCTA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Is likely involved in the aerobic desaturation system responsible for the synthesis of oleic acid from stearoyl-CoA; oleic acid is a precursor of mycobacterial membrane phospholipids and triglycerides. Catalyzes the conversion of stearoyl-CoA to oleoyl-CoA by introduction of a cis double bond between carbons 9 and 10 of the acyl chain. Requires the electron transfer partner Rv3230c to pass two electrons from NADPH to its active site diiron center. Is also able to catalyze the 9-desaturation of palmitoyl-CoA to palmitoleoyl-CoA. {ECO:0000269|PubMed:14559907, ECO:0000269|PubMed:17087501}.

Family

Fatty acid desaturase type 1 family

GO

metal ion binding GO:0046872 molecular_function
plasma membrane GO:0005886 cellular_component
long-chain fatty acid biosynthetic process GO:0042759 biological_process
linoleoyl-CoA desaturase activity GO:0016213 molecular_function

InterPro

IPR005804

UniProt: P9WNZ3
GenBank: Rv3229c
EnsemblBacteria: Rv3229c
Mycobrowser: Rv3229c

Summary

Name: NADPH-dependent stearoyl-CoA 9-desaturase (EC 1.14.19.n4) (Delta(9)-stearoyl desaturase) (Stearoyl-CoA 9-desaturase (NADPH)) (Stearoyl-CoA Delta(9)-desaturase)
Family: Fatty acid desaturase type 1 family
Protein Sequence: MAITDVDVFAHLTDADIENLAAELDAIRRDVEESRGERDARYIRRTIAAQRALEVSGRLLLAGSSRRLAWWTGALTLGVAKIIENMEIGHNVMHGQWDWMNDPEIHSSTWEWDMSGSSKHWRYTHNFVHHKYTNILGMDDDVGYGMLRVTRDQRWKRYNIFNVVWNTILAIGFEWGVALQHLEIGKIFKGRADREAAKTRLREFSAKAGRQVFKDYVAFPALTSLSPGATYRSTLTANVVANVIRNVWSNAVIFCGHFPDGAEKFTKTDMIGEPKGQWYLRQMLGSANFNAGPALRFMSGNLCHQIEHHLYPDLPSNRLHEISVRVREVCDRYDLPYTTGSFLVQYGKTWRTLAKLSLPDKYLRDNADDAPETRSERMFAGLGPGFAGADPVTGRRRGLKTAIAAVRGRRRSKRMAKSVTEPDDLAA
Mass: 48,443 Da
Length: 427 Aa

Rv3229c doesn't seem to be a targeted by any drug.

Biosynthesis of unsaturated fatty acids mtu01040
mtu01040
PATHWAY_MAP mtu01040 Biosynthesis of unsaturated fatty acids

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
In vivo inactivation of the mycobacterial integral membrane stearoyl coenzyme A desaturase DesA3 by a C-terminus-specific degradation process.: J Bacteriol. 2008 Oct;190(20):6686-96. doi: 10.1128/JB.00585-08. Epub 2008 Aug 22.
targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis.: BMC Syst Biol. 2008 Dec 19;2:109. doi: 10.1186/1752-0509-2-109.
Identification of Rv3230c as the NADPH oxidoreductase of a two-protein DesA3 acyl-CoA desaturase in Mycobacterium tuberculosis H37Rv.: Biochemistry. 2006 Nov 14;45(45):13476-86. doi: 10.1021/bi0615285.
Genetic requirements for mycobacterial survival during infection.: Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12989-94. doi: 10.1073/pnas.2134250100. Epub 2003 Oct 20.
Unique mechanism of action of the thiourea drug isoxyl on Mycobacterium tuberculosis.: J Biol Chem. 2003 Dec 26;278(52):53123-30. doi: 10.1074/jbc.M311209200. Epub 2003 Oct 14.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.