Gene - combatTB X

mtrB

Type: protein_coding
Name: mtrB
Locus Name: Rv3245c
Product: Two component sensory transduction histidine kinase MtrB
Functional Category: Regulatory proteins
Location: 3624910..3626613 (- strand)
Gene Length: 1703 bp
Nucleotides: ATGATCTTCGGCTCGCGCCGACGCATTCGGGGTCGCCGGGGTCGCTCTGGCCCGATGACACGGGGCCTCAGTGCGTTGAGTCGAGCCGTAGCTGTCGCGTGGCGCCGATCGCTGCAGCTGCGGGTCGTGGCGCTGACCCTTGGACTGTCGCTAGCCGTCATCCTGGCGCTTGGCTTTGTGCTGACCAGCCAGGTCACCAATCGTGTCCTCGACATCAAGGTCAGGGCGGCGATCGACCAGATCGAGCGGGCACGCACCACCGTCAGCGGGATCGTCAACGGTGAGGAGACGCGCTCACTGGACAGTAGCCTGCAGCTTGCGCGCAACACTCTGACGTCGAAAACCGACCCCGCTTCGGGGGCTGGCCTCGCCGGTGCGTTCGATGCGGTGCTGATGGTGCCGGGTGATGGGCCGCGCGCTGCCTCTACTGCCGGACCCGTCGATCAGGTGCCCAACGCGCTGCGCGGCTTCGTCAAGGCCGGGCAGGCCGCCTACCAGTACGCCACGGTGCAGACCGAAGGTTTCTCCGGGCCGGCCCTGATCATCGGGACTCCGACGTTGTCGCGGGTGGCCAACCTGGAGCTATACCTGATCTTTCCGCTGGCGAGCGAGCAAGCCACGATCACGCTGGTGCGTGGCACGATGGCCACCGGCGGCCTGGTACTGCTAGTCCTGCTCGCCGGCATTGCGCTGCTGGTGTCGCGTCAGGTGGTGGTGCCGGTGCGGTCGGCGTCGCGGATCGCCGAACGTTTCGCCGAGGGACATCTGTCCGAACGCATGCCGGTGCGCGGCGAGGACGACATGGCCAGGCTGGCGGTGTCGTTCAACGACATGGCCGAGAGCCTGTCCCGACAGATCGCCCAGCTGGAGGAGTTCGGCAACCTACAGCGCCGGTTCACCTCCGACGTCAGCCACGAACTGCGTACGCCGCTGACCACGGTGCGGATGGCGGCCGACTTGATCTATGACCACAGCGCCGACCTCGACCCCACGCTGCGGCGGTCCACCGAGTTGATGGTTAGCGAGCTGGACCGATTCGAGACGTTGCTCAACGACCTGCTGGAGATCTCGCGGCATGACGCCGGGGTGGCCGAGTTGTCGGTTGAGGCGGTCGACTTGCGCACAACGGTCAACAACGCGCTCGGCAATGTGGGCCACCTGGCCGAGGAGGCCGGTATCGAGTTGCTGGTGGATCTGCCCGCCGAGCAGGTGATCGCGGAGGTCGATGCGCGTCGGGTAGAACGGATCCTGCGCAATCTGATTGCCAATGCCATCGACCACGCCGAACACAAACCGGTGCGGATCCGGATGGCCGCCGACGAAGACACGGTCGCCGTCACCGTGCGTGACTACGGGGTTGGGCTGCGGCCTGGTGAGGAGAAGCTGGTGTTTAGCCGGTTCTGGCGCTCGGATCCCTCACGGGTACGTCGGTCCGGCGGCACCGGGCTGGGTTTGGCGATCAGCGTCGAGGATGCCCGATTGCACCAGGGTCGGCTTGAGGCGTGGGGCGAACCCGGCGAGGGCGCCTGCTTCCGGCTGACGCTTCCGATGGTGCGCGGCCACAAGGTCACCACCAGCCCGCTGCCCATGAAACCGATCCCGCAGCCAGTTCTGCAACCGGTCGCGCAACCGAATCCGCAACCGATGCCACCGGAATACAAGGAACGTCAGCGCCCACGTGAGCACGCGGAGTGGAGCGGTTG
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Member of the two-component regulatory system MtrA/MtrB. Probably functions as a membrane-associated protein kinase that phosphorylates MtrA in response to environmental signals. Autophosphorylates and transfers phosphate to MtrA in vitro. Overexpression of MtrA alone decreases bacterial virulence in mouse infection; co-expression of MtrA and MtrB restores normal bacterial growth, suggesting that bacterial growth in macrophages requires an optimal ratio of MtrB to MtrA. Probably plays a role in cell division. {ECO:0000269|PubMed:21295603, ECO:0000269|PubMed:22610443}.

Family

Unknown

GO

ATP binding GO:0005524 molecular_function
cytosol GO:0005829 cellular_component
plasma membrane GO:0005886 cellular_component
phosphorelay sensor kinase activity GO:0000155 molecular_function
integral component of membrane GO:0016021 cellular_component

InterPro

UniProt: P9WGK9
GenBank: Rv3245c
EnsemblBacteria: Rv3245c
Mycobrowser: Rv3245c

Summary

Name: Sensor histidine kinase MtrB (EC 2.7.13.3)
Family: Unknown
Protein Sequence: MIFGSRRRIRGRRGRSGPMTRGLSALSRAVAVAWRRSLQLRVVALTLGLSLAVILALGFVLTSQVTNRVLDIKVRAAIDQIERARTTVSGIVNGEETRSLDSSLQLARNTLTSKTDPASGAGLAGAFDAVLMVPGDGPRAASTAGPVDQVPNALRGFVKAGQAAYQYATVQTEGFSGPALIIGTPTLSRVANLELYLIFPLASEQATITLVRGTMATGGLVLLVLLAGIALLVSRQVVVPVRSASRIAERFAEGHLSERMPVRGEDDMARLAVSFNDMAESLSRQIAQLEEFGNLQRRFTSDVSHELRTPLTTVRMAADLIYDHSADLDPTLRRSTELMVSELDRFETLLNDLLEISRHDAGVAELSVEAVDLRTTVNNALGNVGHLAEEAGIELLVDLPAEQVIAEVDARRVERILRNLIANAIDHAEHKPVRIRMAADEDTVAVTVRDYGVGLRPGEEKLVFSRFWRSDPSRVRRSGGTGLGLAISVEDARLHQGRLEAWGEPGEGACFRLTLPMVRGHKVTTSPLPMKPIPQPVLQPVAQPNPQPMPPEYKERQRPREHAEWSG
Mass: 61,637 Da
Length: 567 Aa

Rv3245c doesn't seem to be a targeted by any drug.

Two-component system mtu02020
mtu02020
Environmental Information Processing; Signal transduction

Septal localization of the Mycobacterium tuberculosis MtrB sensor kinase promotes MtrA regulon expression.: J Biol Chem. 2012 Jul 6;287(28):23887-99. doi: 10.1074/jbc.M112.346544. Epub 2012 May 20.
Mycobacterium tuberculosis mtrA merodiploid strains with point mutations in the signal-receiving domain of MtrA exhibit growth defects in nutrient broth.: Plasmid. 2011 May;65(3):210-8. doi: 10.1016/j.plasmid.2011.01.002. Epub 2011 Feb 3.
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
A lipoprotein modulates activity of the MtrAB two-component system to provide intrinsic multidrug resistance, cytokinetic control and cell wall homeostasis in Mycobacterium.: Mol Microbiol. 2010 Apr;76(2):348-64. doi: 10.1111/j.1365-2958.2010.07110.x. Epub 2010 Mar 10.
targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis.: BMC Syst Biol. 2008 Dec 19;2:109. doi: 10.1186/1752-0509-2-109.
Modulation of Mycobacterium tuberculosis proliferation by MtrA, an essential two-component response regulator.: Mol Microbiol. 2006 May;60(3):643-57. doi: 10.1111/j.1365-2958.2006.05137.x.
An essential two-component signal transduction system in Mycobacterium tuberculosis.: J Bacteriol. 2000 Jul;182(13):3832-8. doi: 10.1128/jb.182.13.3832-3838.2000.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.
Elements of signal transduction in Mycobacterium tuberculosis: in vitro phosphorylation and in vivo expression of the response regulator MtrA.: J Bacteriol. 1996 Jun;178(11):3314-21. doi: 10.1128/jb.178.11.3314-3321.1996.