Gene - combatTB X

dnaE2

Type: protein_coding
Name: dnaE2
Locus Name: Rv3370c
Product: Probable DNA polymerase III (alpha chain) DnaE2 (DNA nucleotidyltransferase)
Functional Category: Information pathways
Location: 3781501..3784740 (- strand)
Gene Length: 3239 bp
Nucleotides: ATGGAACGGGTGCTTAACGGCAAGCCGCGCCATGCCGGGGTGCCGGCGTTCGACGCGGATGGGGACGTCCCACGGTCCCGCAAGCGCGGGGCGTACCAGCCGCCGGGCCGGGAACGGGTCGGATCGTCCGTCGCGTATGCCGAGCTGCATGCGCATTCGGCGTACAGCTTCCTCGACGGGGCCAGCACGCCCGAGGAGTTGGTCGAGGAGGCCGCCCGGCTGGGCCTGTGTGCCCTGGCGCTGACCGACCACGACGGCCTGTACGGGGCGGTGCGGTTCGCCGAAGCGGCCGCGGAACTCGACGTGCGCACGGTCTTCGGCGCCGAGCTGTCACTGGGCGCCACGGCTCGCACCGAGCGGCCGGATCCGCCCGGCCCGCACCTGCTGGTGTTGGCCCGCGGCCCGGAAGGCTACCGGCGGTTGTCGCGGCAACTGGCGGCGGCGCATCTGGCCGGCGGCGAAAAGGGTAAGCCGCGCTACGACTTCGACGCGCTAACCGAGGCCGCCGGCGGGCACTGGCACATCCTGACTGGCTGCCGCAAAGGCCATGTGCGCCAAGCGCTTTCCCAGGGTGGCCCGGCCGCGGCGCAGCGGGCGCTGGCCGATCTGGTGGACCGGTTCACCCCCAGCCGGGTCAGCATCGAGCTGACCCATCATGGTCACCCGCTCGACGACGAACGCAACGCCGCGCTGGCCGGACTGGCGCCGCGCTTCGGTGTCGGCATCGTCGCCACCACCGGGGCGCACTTCGCGGATCCGTCACGTGGGCGGCTGGCGATGGCGATGGCGGCCATCCGGGCCCGGCGGTCGCTGGACTCCGCGGCCGGGTGGCTGGCTCCGCTGGGTGGGGCGCACCTGCGGTCCGGGGAGGAGATGGCCCGGTTGTTCGCGTGGTGCCCCGAGGCGGTGACCGCGGCCGCCGAGCTCGGCGAGCGGTGCGCCTTTGGCCTACAGCTCATCGCGCCGCGGCTGCCGCCGTTCGATGTTCCCGACGGGCACACCGAGGACAGCTGGCTGCGGTCGTTGGTCATGGCGGGCGCCCGCGAGCGTTACGGGCCGCCCAAGAGCGCGCCCCGGGCGTACTCCCAGATCGAGCATGAGCTGAAAGTCATTGCCCAACTGAGATTTCCGGGCTACTTCCTGGTGGTGCACGACATCACCCGGTTTTGCCGGGACAACGACATCCTGTGTCAGGGCAGGGGATCGGCGGCCAACTCCGCGGTCTGCTATGCCCTGGGCGTCACCGCCGTCGACCCGGTGGCCAACGAGCTGTTGTTCGAGCGCTTCTTATCGCCCGCCCGCGACGGGCCACCCGACATCGACATCGACATCGAGTCGGATCAGCGCGAAAAGGTCATCCAGTACGTCTACCACAAATACGGCCGCGACTACGCCGCCCAGGTCGCCAACGTCATCACCTACCGGGGGCGCAGCGCGGTGCGTGACATGGCCCGCGCCCTGGGCTTCTCGCCGGGCCAGCAGGACGCGTGGAGCAAGCAGGTCAGCCACTGGACCGGGCAGGCCGACGACGTTGACGGCATCCCCGAGCAGGTGATCGACCTGGCCACCCAGATCCGCAACCTGCCGCGGCACCTAGGCATCCACTCCGGCGGCATGGTCATCTGCGACCGCCCGATCGCCGACGTGTGCCCGGTGGAATGGGCGCGCATGGCCAACCGCAGCGTCCTGCAATGGGACAAAGACGACTGCGCGGCAATCGGCTTGGTGAAATTCGACCTACTCGGGCTGGGCATGCTCTCGGCGCTGCACTATGCGAAAGACCTGGTGGCCGAGCACAAGGGCATCGAGGTGGACCTGGCCCGCCTCGACCTCTCCGAGCCGGCGGTGTATGAGATGCTGGCCCGCGCCGATTCCGTCGGCGTGTTCCAGGTGGAGTCACGTGCGCAGATGGCCACCCTGCCGCGCCTTAAGCCGCGAGTGTTCTACGACCTGGTGGTGGAGGTGGCGCTGATCCGCCCCGGGCCCATCCAGGGCGGATCGGTGCACCCCTACATCCGGCGGCGCAACGGCGTCGACCCGGTCATCTACGAGCACCCGTCGATGGCGCCGGCATTGCGAAAGACGCTGGGAGTGCCGCTGTTTCAGGAGCAGCTGATGCAGTTGGCGGTCGACTGCGCCGGCTTTTCCGCCGCCGAGGCCGACCAGCTGCGCCGCGCCATGGGGTCCAAACGCTCCACCGAACGCATGCGCCGGCTGCGCGGCCGGTTCTACGACGGCATGCGCGCGCTGCACGGCGCCCCCGACGAGGTGATCGACCGGATCTACGAAAAGCTGGAGGCGTTTGCCAATTTCGGCTTCCCCGAGAGCCACGCACTGAGTTTCGCGTCGCTGGTGTTCTACTCGGCGTGGTTCAAGCTGCACCACCCGGCGGCGTTCTGTGCGGCGCTGCTGCGCGCCCAGCCGATGGGTTTCTATTCGCCGCAGTCGCTGGTGGCCGACGCCCGCCGGCACGGCGTGGCGGTGCACGGCCCGTGTGTCAATGCCAGCCTGGCGCACGCCACGTGTGAGAACGCCGGAACGGAGGTGCGTTTGGGCTTGGGCGCCGTCCGCTACCTCGGCGCCGAGCTGGCCGAGAAGCTGGTCGCGGAGCGAACAGCCAACGGCCCGTTCACCTCCCTGCCGGACTTGACGTCGCGGGTGCAGCTTTCCGTGCCGCAGGTCGAAGCGCTGGCGACGGCCGGGGCGCTGGGCTGCTTCGGCATGTCTCGGCGGGAGGCGCTGTGGGCGGCCGGGGCCGCGGCCACGGGCCGGCCGGACCGGTTACCCGGGGTGGGCTCGTCGTCGCACATCCCGGCGCTGCCGGGGATGAGCGAGCTGGAGCTGGCCGCCGCCGACGTGTGGGCCACCGGCGTCTCCCCGGACAGCTATCCGACCCAGTTCCTGCGGGCGGACCTGGACGCGATGGGGGTGCTGCCCGCCGAGAGGCTGGGATCGGTGTCCGACGGCGACCGCGTGCTGATCGCCGGCGCGGTGACTCATCGGCAGCGACCCGCCACGGCCCAGGGGGTGACGTTCATCAACCTCGAAGACGAGACCGGGATGGTCAACGTGCTCTGCACGCCCGGGGTGTGGGCGCGACACCGCAAGCTCGCGCACACGGCGCCGGCGCTGCTGATCCGCGGCCAGGTCCAAAACGCCAGCGGCGCAATCACCGTCGTCGCCGAACGGATGGGCCGCCTCACCCTGGCGGTCGGCGCGCGCTCGCGCGACTTCCGGTG
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase. Does not appear to be essential for chromosomal replication. May be involved in generating antibiotic resistance. {ECO:0000269|PubMed:12705867}.

Family

DNA polymerase type-C family, DnaE2 subfamily

GO

response to antibiotic GO:0046677 biological_process
DNA-directed DNA polymerase activity GO:0003887 molecular_function
error-prone translesion synthesis GO:0042276 biological_process
cell wall GO:0005618 cellular_component
cytoplasm GO:0005737 cellular_component
plasma membrane GO:0005886 cellular_component
DNA repair GO:0006281 biological_process
DNA replication GO:0006260 biological_process
3'-5' exonuclease activity GO:0008408 molecular_function

InterPro

UniProt: P9WNT5
GenBank: Rv3370c
EnsemblBacteria: Rv3370c
Mycobrowser: Rv3370c

Summary

Name: Error-prone DNA polymerase (EC 2.7.7.7)
Family: DNA polymerase type-C family, DnaE2 subfamily
Protein Sequence: MFDILWNVGWSNGPPSWAEMERVLNGKPRHAGVPAFDADGDVPRSRKRGAYQPPGRERVGSSVAYAELHAHSAYSFLDGASTPEELVEEAARLGLCALALTDHDGLYGAVRFAEAAAELDVRTVFGAELSLGATARTERPDPPGPHLLVLARGPEGYRRLSRQLAAAHLAGGEKGKPRYDFDALTEAAGGHWHILTGCRKGHVRQALSQGGPAAAQRALADLVDRFTPSRVSIELTHHGHPLDDERNAALAGLAPRFGVGIVATTGAHFADPSRGRLAMAMAAIRARRSLDSAAGWLAPLGGAHLRSGEEMARLFAWCPEAVTAAAELGERCAFGLQLIAPRLPPFDVPDGHTEDSWLRSLVMAGARERYGPPKSAPRAYSQIEHELKVIAQLRFPGYFLVVHDITRFCRDNDILCQGRGSAANSAVCYALGVTAVDPVANELLFERFLSPARDGPPDIDIDIESDQREKVIQYVYHKYGRDYAAQVANVITYRGRSAVRDMARALGFSPGQQDAWSKQVSHWTGQADDVDGIPEQVIDLATQIRNLPRHLGIHSGGMVICDRPIADVCPVEWARMANRSVLQWDKDDCAAIGLVKFDLLGLGMLSALHYAKDLVAEHKGIEVDLARLDLSEPAVYEMLARADSVGVFQVESRAQMATLPRLKPRVFYDLVVEVALIRPGPIQGGSVHPYIRRRNGVDPVIYEHPSMAPALRKTLGVPLFQEQLMQLAVDCAGFSAAEADQLRRAMGSKRSTERMRRLRGRFYDGMRALHGAPDEVIDRIYEKLEAFANFGFPESHALSFASLVFYSAWFKLHHPAAFCAALLRAQPMGFYSPQSLVADARRHGVAVHGPCVNASLAHATCENAGTEVRLGLGAVRYLGAELAEKLVAERTANGPFTSLPDLTSRVQLSVPQVEALATAGALGCFGMSRREALWAAGAAATGRPDRLPGVGSSSHIPALPGMSELELAAADVWATGVSPDSYPTQFLRADLDAMGVLPAERLGSVSDGDRVLIAGAVTHRQRPATAQGVTFINLEDETGMVNVLCTPGVWARHRKLAHTAPALLIRGQVQNASGAITVVAERMGRLTLAVGARSRDFR
Mass: 118,672 Da
Length: 1098 Aa

Rv3370c doesn't seem to be a targeted by any drug.

Rv3370c doesn't seem to be involved in any pathway.

DnaE2 polymerase contributes to in vivo survival and the emergence of drug resistance in Mycobacterium tuberculosis.: Cell. 2003 Apr 18;113(2):183-93.
Definition of the mycobacterial SOS box and use to identify LexA-regulated genes in Mycobacterium tuberculosis.: J Bacteriol. 2002 Jun;184(12):3287-95. doi: 10.1128/jb.184.12.3287-3295.2002.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.