Gene - combatTB X

rmlB

Type: protein_coding
Name: rmlB
Locus Name: Rv3464
Product: dTDP-glucose 4,6-dehydratase RmlB
Functional Category: Intermediary metabolism and respiration
Location: 3881837..3882832 (+ strand)
Gene Length: 995 bp
Nucleotides: TGCGGTTGCTAGTCACCGGTGGCGCGGGATTCATCGGCACGAATTTCGTGCACAGCGCCGTACGTGAGCATCCAGACGATGCGGTTACCGTACTCGACGCCCTGACCTACGCCGGCCGGCGCGAGTCGCTGGCCGACGTGGAGGATGCCATCCGGCTGGTTCAGGGCGATATCACCGACGCCGAGCTGGTTTCGCAGCTGGTGGCCGAGTCCGACGCGGTGGTGCATTTTGCCGCCGAATCCCATGTCGACAATGCACTGGACAATCCGGAGCCGTTTCTGCACACCAACGTCATCGGGACCTTCACCATCCTGGAAGCGGTGCGACGCCACGGTGTGCGCCTGCACCACATCTCCACCGACGAGGTCTACGGCGACTTGGAGCTCGACGACCGGGCGCGGTTCACCGAATCGACGCCCTATAACCCGTCCAGCCCTTACTCGGCGACCAAGGCGGGCGCAGACATGTTGGTCCGGGCCTGGGTTCGGTCCTATGGCGTACGCGCGACGATCTCCAACTGCTCCAACAACTACGGGCCGTATCAGCACGTCGAGAAGTTCATTCCGCGTCAGATCACCAATGTGCTCACCGGGCGGCGGCCCAAGCTCTACGGCGCGGGCGCCAATGTCCGTGACTGGATCCACGTCGACGACCACAACAGCGCGGTGCGGCGAATCCTGGACAGAGGCCGCATCGGCCGAACCTACCTGATCAGCTCCGAGGGCGAGCGTGACAACCTGACCGTGCTGCGCACGCTGCTGCGACTGATGGACCGCGATCCGGACGACTTCGACCACGTCACCGACCGCGTCGGCCACGACCTGCGCTATGCCATCGACCCGTCCACGCTCTACGACGAATTATGCTGGGCGCCAAAGCATACCGATTTCGAGGAGGGCCTGCGGACCACGATCGACTGGTACCGCGACAACGAATCGTGGTGGCGTCCACTAAAAGACGCCACGGAGGCCCGCTATCAAGAACGCGGTCAATGA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction (By similarity). Involved in the biosynthesis of the dTDP-L-rhamnose which is a component of the critical linker, D-N-acetylglucosamine-L-rhamnose disaccharide, which connects the galactan region of arabinogalactan to peptidoglycan via a phosphodiester linkage (PubMed:16472764). {ECO:0000250|UniProtKB:P27830, ECO:0000269|PubMed:16472764}.

Family

NAD(P)-dependent epimerase/dehydratase family, dTDP-glucose dehydratase subfamily

GO

dTDP-rhamnose biosynthetic process GO:0019305 biological_process
cell wall GO:0005618 cellular_component
NADH binding GO:0070404 molecular_function
dTDP-glucose 4,6-dehydratase activity GO:0008460 molecular_function
extracellular polysaccharide biosynthetic process GO:0045226 biological_process

InterPro

UniProt: P9WN65
GenBank: Rv3464
EnsemblBacteria: Rv3464
Mycobrowser: Rv3464

Summary

Name: dTDP-glucose 4,6-dehydratase (EC 4.2.1.46)
Family: NAD(P)-dependent epimerase/dehydratase family, dTDP-glucose dehydratase subfamily
Protein Sequence: MRLLVTGGAGFIGTNFVHSAVREHPDDAVTVLDALTYAGRRESLADVEDAIRLVQGDITDAELVSQLVAESDAVVHFAAESHVDNALDNPEPFLHTNVIGTFTILEAVRRHGVRLHHISTDEVYGDLELDDRARFTESTPYNPSSPYSATKAGADMLVRAWVRSYGVRATISNCSNNYGPYQHVEKFIPRQITNVLTGRRPKLYGAGANVRDWIHVDDHNSAVRRILDRGRIGRTYLISSEGERDNLTVLRTLLRLMDRDPDDFDHVTDRVGHDLRYAIDPSTLYDELCWAPKHTDFEEGLRTTIDWYRDNESWWRPLKDATEARYQERGQ
Mass: 37,558 Da
Length: 331 Aa

Rv3464 doesn't seem to be a targeted by any drug.

Streptomycin biosynthesis mtu00521
mtu00521
Metabolism; Biosynthesis of other secondary metabolites

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
rmlB and rmlC genes are essential for growth of mycobacteria.: Biochem Biophys Res Commun. 2006 Mar 31;342(1):170-8. doi: 10.1016/j.bbrc.2006.01.130. Epub 2006 Feb 3.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.