Gene - combatTB X

fadE26

Type: protein_coding
Name: fadE26
Locus Name: Rv3504
Product: Probable acyl-CoA dehydrogenase FadE26
Functional Category: Lipid metabolism
Location: 3922471..3923673 (+ strand)
Gene Length: 1202 bp
Nucleotides: TGCGCATCAGTTACACCCCGCAGCAGGAGGAGCTGCGCCGCGAGCTGCGCTCGTACTTTGCCACGTTGATGACGCCGGAACGCCGGGAGGCGCTGAGCTCGGTCCAGGGTGAATACGGCGTCGGCAATGTCTACCGGGAGACGATCGCGCAAATGGGCCGCGACGGGTGGCTTGCGCTGGGCTGGCCCAAGGAATACGGCGGCCAGGGCCGCTCGGCGATGGACCAGCTGATCTTCACCGATGAAGCCGCCATCGCCGGTGCACCGGTGCCGTTCCTGACCATCAACAGCGTGGCGCCGACGATCATGGCCTACGGAACCGACGAGCAGAAGAGGTTTTTCCTGCCCCGGATCGCCGCCGGGGATCTGCACTTCTCGATCGGCTACTCCGAGCCCGGCGCCGGCACCGACCTGGCCAACCTGCGCACCACCGCGGTTCGCGACGGCGATGACTATGTGGTCAACGGCCAGAAGATGTGGACCAGCCTGATTCAGTACGCCGACTACGTCTGGTTAGCGGTACGCACCAACCCGGAGTCTTCTGGGGCCAAAAAACACCGTGGCATATCGGTGTTAATCGTGCCGACGACCGCTGAGGGCTTCTCCTGGACTCCAGTGCACACCATGGCCGGTCCGGACACCAGCGCCACCTACTACTCCGACGTGCGGGTACCGGTGGCCAACCGGGTCGGTGAGGAAAACGCCGGCTGGAAGCTGGTGACCAACCAGCTCAACCACGAGCGGGTCGCCCTGGTGTCGCCGGCACCGATTTTCGGATGCCTGCGCGAGGTCCGCGAATGGGCACAAAACACCAAGGACGCCGGCGGCACCAGGCTGATCGACTCGGAGTGGGTGCAGCTCAACCTGGCCCGGGTACACGCCAAGGCCGAAGTCCTCAAGCTGATCAACTGGGAGCTGGCTTCCTCGCAAAGTGGGCCGAAGGACGCTGGACCGTCACCGGCCGATGCGTCGGCGGCCAAGGTGTTCGGTACCGAGCTGGCCACCGAGGCCTACCGGCTGCTGATGGAGGTGTTGGGCACTGCGGCGACCCTGCGCCAGAATTCGCCAGGCGCGTTGCTGCGCGGCCGCGTCGAACGGATGCACCGGGCGTGCCTGATCCTGACGTTCGGCGGCGGCACCAACGAAGTCCAGCGCGACATCATCGGCATGGTCGCGCTGGGACTGCCGCGAGCCAACCGCTGA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Involved in the first cycle of side chain dehydrogenation in the beta-oxidation of cholesterol catabolism (PubMed:26161441). It contributes partly to the virulence by increasing the efficiency of beta-oxidation. Catalyzes the dehydrogenation of acyl-CoA ester side chains of (25S)-3-oxo-cholest-4-en-26-oyl-CoA (3-OCS-CoA) to yield (24E)-3-oxo-cholest-4,24-dien-26-oyl-CoA (PubMed:26348625, PubMed:26161441). Also able to dehydrogenate steroyl-CoA such as 3-oxo-chol-4-en-24-oyl-CoA (3-OCO-CoA) as well as 3-oxo-4-pregnene-20-carboxyl-CoA (3-OPC-CoA) (PubMed:26161441). It dehydrogenates only (25S)-OCS-CoA diastereomer (Probable). {ECO:0000269|PubMed:26161441, ECO:0000269|PubMed:26348625}.

Family

Acyl-CoA dehydrogenase family

GO

pathogenesis GO:0009405 biological_process
flavin adenine dinucleotide binding GO:0050660 molecular_function
oxidoreductase activity, acting on the CH-CH group of donors GO:0016627 molecular_function
cholesterol catabolic process GO:0006707 biological_process

InterPro

UniProt: I6YCA3
GenBank: Rv3504
EnsemblBacteria: Rv3504
Mycobrowser: Rv3504

Summary

Name: Acyl-CoA dehydrogenase FadE26 (ACAD) (EC 1.3.99.-) (3-oxocholest-4-en-26-oyl-CoA dehydrogenase alpha subunit)
Family: Acyl-CoA dehydrogenase family
Protein Sequence: MRISYTPQQEELRRELRSYFATLMTPERREALSSVQGEYGVGNVYRETIAQMGRDGWLALGWPKEYGGQGRSAMDQLIFTDEAAIAGAPVPFLTINSVAPTIMAYGTDEQKRFFLPRIAAGDLHFSIGYSEPGAGTDLANLRTTAVRDGDDYVVNGQKMWTSLIQYADYVWLAVRTNPESSGAKKHRGISVLIVPTTAEGFSWTPVHTMAGPDTSATYYSDVRVPVANRVGEENAGWKLVTNQLNHERVALVSPAPIFGCLREVREWAQNTKDAGGTRLIDSEWVQLNLARVHAKAEVLKLINWELASSQSGPKDAGPSPADASAAKVFGTELATEAYRLLMEVLGTAATLRQNSPGALLRGRVERMHRACLILTFGGGTNEVQRDIIGMVALGLPRANR
Mass: 43,785 Da
Length: 400 Aa

Rv3504 doesn't seem to be a targeted by any drug.

Rv3504 doesn't seem to be involved in any pathway.

Unraveling Cholesterol Catabolism in Mycobacterium tuberculosis: ChsE4-ChsE5 alpha2beta2 Acyl-CoA Dehydrogenase Initiates beta-Oxidation of 3-Oxo-cholest-4-en-26-oyl CoA.: ACS Infect Dis. 2015 Feb 13;1(2):110-125. doi: 10.1021/id500033m. Epub 2015 Jan 8.
alpha-Methyl Acyl CoA Racemase Provides Mycobacterium tuberculosis Catabolic Access to Cholesterol Esters.: Biochemistry. 2015 Sep 22;54(37):5669-72. doi: 10.1021/acs.biochem.5b00911. Epub 2015 Sep 11.
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
A highly conserved transcriptional repressor controls a large regulon involved in lipid degradation in Mycobacterium smegmatis and Mycobacterium tuberculosis.: Mol Microbiol. 2007 Aug;65(3):684-99. doi: 10.1111/j.1365-2958.2007.05827.x.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.