fadE29
- Type
- protein_coding
- Name
- fadE29
- Locus Name
-
Rv3543c
- Product
-
Probable acyl-CoA dehydrogenase FadE29
- Functional Category
-
Lipid metabolism
- Location
- 3981977..3983140 (- strand)
- Gene Length
- 1163 bp
- Nucleotides
-
ATGTTCATTGACCTGACGCCGGAGCAGCGCCAGCTGCAAGCCGAGATACGGCAATACTTTTCGAATCTCATCTCGCCCGACGAGCGCACGGAGATGGAGAAAGACCGGCACGGCCCGGCCTACCGTGCGGTGATACGGCGCATGGGCCGGGACGGCAGGCTCGGTGTTGGGTGGCCGAAAGAGTTCGGCGGTCTGGGTTTTGGTCCGATCGAGCAGCAGATCTTCGTCAACGAGGCCCATCGCGCCGACGTGCCGCTGCCCGCGGTGACGCTGCAGACCGTCGGCCCCACGCTGCAGGCGCACGGCAGCGAGCTGCAGAAGAAGAAGTTCCTGCCGGCGATCCTGGCCGGTGAGGCGCACTTCGCGATCGGCTACACCGAGCCGGAAGCCGGCACCGACCTGGCGTCGTTGCGCACCACCGCCGTTCGCGACGGCGACCACTACATCGTCAACGGCCAGAAGGTCTTCACCACCGGCGCACACGACGCCGACTACATCTGGCTGGCCTGCCGCACCGACCCGAATGCCGCTAAGCACAAAGGCATTTCCATTCTAATCGTCGACACCAAGGATCCCGGCTATTCCTGGACGCCGATCATCTTGGCCGACGGCGCCCACCACACCAATGCCACGTACTACAACGACGTGCGCGTGCCGGTCGACATGCTGGTCGGAAAGGAGAACGACGGCTGGCGGCTGATCACCACCCAACTCAACAACGAACGGGTAATGCTCGGCCCGGCCGGGCGGTTCGCCAGCATCTACGACCGGGTGCACGCGTGGGCGTCCGTGCCGGGTGGCAACGGCGTGACGCCGATCGACCACGACGACGTCAAGCGGGCTCTTGGTGAGATTCGTGCGATCTGGCGGATCAACGAGTTGCTCAACTGGCAGGTAGCGTCCGCCGGTGAGGACATCAACATGGCCGATGCCGCGGCCACGAAAGTCTTTGGCACCGAGCGTGTTCAGCGTGCCGGCCGGCTCGCCGAGGAAATCGTTGGCAAGTACGGCAACCCCGCCGAACCCGACACCGCCGAGCTACTGCGCTGGCTGGACGCCCAGACCAAGCGCAACCTGGTGATCACCTTCGGCGGGGGTGTGAACGAAGTCATGCGCGAGATGATCGCCGCGTCTGGCCTCAAAGTGCCAAGGGTGCCCAGGTG
- Drug Resistance
- Mutations
- Function
- Involved in the third cycle of side chain dehydrogenation in the beta-oxidation of cholesterol catabolism (PubMed:26161441). Contributes partly to the virulence by increasing the efficiency of beta-oxidation (PubMed:22045806, PubMed:23560677). Catalyzes the dehydrogenation of 2'-propanoyl-CoA ester side chains of 3-oxo-4-pregnene-20-carboxyl-CoA (3-OPC-CoA) to yield 3-oxo-4,17-pregnadiene-20-carboxyl-CoA (3-OPDC-CoA). Also able to dehydrogenate steroyl-CoA such as 3-oxo-chol-4-en-24-oyl-CoA (3-OCO-CoA), 1beta-(2'-propanoyl-CoA)-3a-alpha-H- 7a-beta-methylhexahydro-4-indanone (indanone-CoA ester), hexahydroindanone and pregenenone (PubMed:22045806, PubMed:23560677). {ECO:0000269|PubMed:22045806, ECO:0000269|PubMed:23560677, ECO:0000269|PubMed:26161441}.
- Family
-
Acyl-CoA dehydrogenase family
- GO
-
- FAD binding GO:0071949 molecular_function
- growth of symbiont in host GO:0044117 biological_process
- pathogenesis GO:0009405 biological_process
- cholesterol catabolic process GO:0006707 biological_process
- oxidoreductase activity, acting on the CH-CH group of donors GO:0016627 molecular_function
- fatty acid beta-oxidation using acyl-CoA dehydrogenase GO:0033539 biological_process
- InterPro
Summary
- Name
- Acyl-CoA dehydrogenase FadE29 (ACAD) (EC 1.3.99.-) (3-oxo-23,24-bisnorchol-4-en-22-oyl-CoA dehydrogenase beta subunit) (3-oxo-4-pregnene-20-carboxyl-CoA dehydrogenase beta subunit)
- Family
- Acyl-CoA dehydrogenase family
- Protein Sequence
-
MFIDLTPEQRQLQAEIRQYFSNLISPDERTEMEKDRHGPAYRAVIRRMGRDGRLGVGWPKEFGGLGFGPIEQQIFVNEAHRADVPLPAVTLQTVGPTLQAHGSELQKKKFLPAILAGEAHFAIGYTEPEAGTDLASLRTTAVRDGDHYIVNGQKVFTTGAHDADYIWLACRTDPNAAKHKGISILIVDTKDPGYSWTPIILADGAHHTNATYYNDVRVPVDMLVGKENDGWRLITTQLNNERVMLGPAGRFASIYDRVHAWASVPGGNGVTPIDHDDVKRALGEIRAIWRINELLNWQVASAGEDINMADAAATKVFGTERVQRAGRLAEEIVGKYGNPAEPDTAELLRWLDAQTKRNLVITFGGGVNEVMREMIAASGLKVPRVPR
- Mass
- 42,715 Da
- Length
- 387 Aa
Rv3543c doesn't seem to be a targeted by any drug.
Rv3543c doesn't seem to be involved in any pathway.
- Unraveling Cholesterol Catabolism in Mycobacterium tuberculosis: ChsE4-ChsE5 alpha2beta2 Acyl-CoA Dehydrogenase Initiates beta-Oxidation of 3-Oxo-cholest-4-en-26-oyl CoA.
- ACS Infect Dis. 2015 Feb 13;1(2):110-125. doi: 10.1021/id500033m. Epub 2015 Jan 8.
- Mycobacterium tuberculosis utilizes a unique heterotetrameric structure for dehydrogenation of the cholesterol side chain.
- Biochemistry. 2013 Apr 30;52(17):2895-904. doi: 10.1021/bi4002979. Epub 2013 Apr 18.
- Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.
- Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
- Pathway profiling in Mycobacterium tuberculosis: elucidation of cholesterol-derived catabolite and enzymes that catalyze its metabolism.
- J Biol Chem. 2011 Dec 23;286(51):43668-78. doi: 10.1074/jbc.M111.313643. Epub 2011 Nov 1.
- A highly conserved transcriptional repressor controls a large regulon involved in lipid degradation in Mycobacterium smegmatis and Mycobacterium tuberculosis.
- Mol Microbiol. 2007 Aug;65(3):684-99. doi: 10.1111/j.1365-2958.2007.05827.x.
- Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.
- Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.