hsaD
- Type
- protein_coding
- Name
- hsaD
- Locus Name
-
Rv3569c
- Product
-
4,9-DHSA hydrolase
- Functional Category
-
Intermediary metabolism and respiration
- Location
- 4010196..4011071 (- strand)
- Gene Length
- 875 bp
- Nucleotides
-
ATGACAGCTACCGAGGAATTGACGTTCGAATCCACCTCGCGCTTTGCGGAAGTGGACGTCGACGGGCCGCTGAAACTGCACTACCACGAGGCCGGCGTGGGCAACGACCAGACGGTGGTGCTACTGCACGGCGGTGGGCCCGGCGCGGCGAGCTGGACGAACTTCTCGCGTAATATCGCGGTGCTGGCGCGGCACTTTCATGTGCTGGCCGTCGACCAGCCCGGTTACGGCCATTCCGACAAGCGGGCCGAGCACGGCCAGTTCAATCGCTATGCCGCGATGGCGCTGAAGGGGCTCTTCGATCAGCTGGGGCTGGGGCGGGTACCGCTGGTGGGCAACTCGTTGGGCGGGGGAACCGCGGTCCGGTTTGCGCTGGACTACCCGGCCCGGGCAGGACGGTTAGTGCTGATGGGCCCGGGGGGCCTGAGTATCAACCTGTTTGCGCCCGACCCGACCGAGGGAGTCAAACGGCTGTCGAAGTTCTCCGTTGCGCCCACCCGGGAGAACCTCGAGGCGTTCCTGCGGGTCATGGTCTACGACAAGAACCTGATCACCCCCGAGTTGGTGGATCAGCGGTTTGCGCTGGCCAGCACCCCGGAGTCGTTGACGGCAACACGGGCGATGGGAAAGTCGTTCGCCGGAGCCGACTTCGAGGCCGGCATGATGTGGCGCGAGGTGTATCGGCTGCGCCAGCCGGTGTTGCTGATCTGGGGTCGTGAGGACCGGGTCAACCCGCTGGACGGCGCGCTGGTTGCGTTGAAAACGATTCCGCGTGCGCAGCTGCACGTATTCGGGCAGTGTGGGCATTGGGTGCAGGTGGAGAAGTTCGACGAGTTCAACAAGCTGACGATTGAATTTCTGGGAGGTGGCAGATG
- Drug Resistance
- Mutations
- Function
- Catalyzes the hydrolysis of a carbon-carbon bond in 4,5: 9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate (4,9-DSHA) to yield 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oate (DOHNAA) and 2-hydroxy-hexa-2,4-dienoate (HHD). Is also able to catalyze the hydrolysis of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) and the synthetic analog 8-(2-chlorophenyl)-2-hydroxy-5-methyl-6-oxoocta-2,4-dienoic acid (HOPODA). {ECO:0000269|PubMed:19875455}.
- Family
-
AB hydrolase superfamily, HsaD family
- GO
-
- aromatic compound catabolic process GO:0019439 biological_process
- growth of symbiont in host GO:0044117 biological_process
- 4,9-DSHA hydrolase activity GO:0034820 molecular_function
- cell wall GO:0005618 cellular_component
- plasma membrane GO:0005886 cellular_component
- 4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase activity GO:0102296 molecular_function
- 2,6-dioxo-6-phenylhexa-3-enoate hydrolase activity GO:0018774 molecular_function
- lipid catabolic process GO:0016042 biological_process
- steroid biosynthetic process GO:0006694 biological_process
- InterPro
Summary
- Name
- 4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase (EC 3.7.1.17) (2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase) (HOPDA hydrolase) (EC 3.7.1.8) (Meta-cleavage product hydrolase) (MCP hydrolase)
- Family
- AB hydrolase superfamily, HsaD family
- Protein Sequence
-
MTATEELTFESTSRFAEVDVDGPLKLHYHEAGVGNDQTVVLLHGGGPGAASWTNFSRNIAVLARHFHVLAVDQPGYGHSDKRAEHGQFNRYAAMALKGLFDQLGLGRVPLVGNSLGGGTAVRFALDYPARAGRLVLMGPGGLSINLFAPDPTEGVKRLSKFSVAPTRENLEAFLRVMVYDKNLITPELVDQRFALASTPESLTATRAMGKSFAGADFEAGMMWREVYRLRQPVLLIWGREDRVNPLDGALVALKTIPRAQLHVFGQCGHWVQVEKFDEFNKLTIEFLGGGR
- Mass
- 31,875 Da
- Length
- 291 Aa
Rv3569c doesn't seem to be a targeted by any drug.
-
-
Steroid degradation mtu00984
mtu00984PATHWAY_MAP mtu00984 Steroid degradation
-
- Characterization of a carbon-carbon hydrolase from Mycobacterium tuberculosis involved in cholesterol metabolism.
- J Biol Chem. 2010 Jan 1;285(1):434-43. doi: 10.1074/jbc.M109.058081. Epub 2009 Oct 29.
- Structure of HsaD, a steroid-degrading hydrolase, from Mycobacterium tuberculosis.
- Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Jan 1;64(Pt 1):2-7. doi: 10.1107/S1744309107065931. Epub 2007 Dec 20.
- Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.
- Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
- A gene cluster encoding cholesterol catabolism in a soil actinomycete provides insight into Mycobacterium tuberculosis survival in macrophages.
- Proc Natl Acad Sci U S A. 2007 Feb 6;104(6):1947-52. doi: 10.1073/pnas.0605728104. Epub 2007 Jan 30.
- A highly conserved transcriptional repressor controls a large regulon involved in lipid degradation in Mycobacterium smegmatis and Mycobacterium tuberculosis.
- Mol Microbiol. 2007 Aug;65(3):684-99. doi: 10.1111/j.1365-2958.2007.05827.x.
- Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.
- Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.