Gene - combatTB X

fadE34

Type: protein_coding
Name: fadE34
Locus Name: Rv3573c
Product: Probable acyl-CoA dehydrogenase FadE34
Functional Category: Lipid metabolism
Location: 4014077..4016212 (- strand)
Gene Length: 2135 bp
Nucleotides: GTGGTAGCGACCGTCACCGACGAACAATCCGCGGCTCGTGAGTTGGTGCGCGGCTGGGCCCGCACCGCGGCCTCAGGTGCGGCCGCGACTGCGGCGGTCCGCGACATGGAATACGGCTTCGAAGAAGGAAATGCGGACGCATGGCGGCCGGTGTTCGCCGGGCTGGCGGGCCTGGGCCTCTTCGGTGTCGCCGTCCCAGAGGATTGTGGCGGAGCTGGCGGCAGCATCGAGGACCTGTGTGCGATGGTCGACGAGGCGGCCAGGGCGTTGGTACCGGGGCCGGTCGCGACCACCGCGGTGGCCACTTTGGTTGTCTCCGATCCCAAGCTGCGCAGCGCGCTAGCGTCGGGTGAACGGTTCGCCGGGGTGGCTATCGACGGTGGCGTGCAGGTCGACCCGAAGACGTCGACCGCGTCGGGCACCGTCGGCCGAGTGCTTGGTGGCGCGCCCGGCGGCGTCGTGCTGTTGCCGGCCGACGGGAATTGGCTGCTGGTGGACACTGCTTGCGACGAGGTCGTAGTGGAGCCGCTGCGGGCCACCGACTTTTCGCTGCCGCTGGCCCGGATGGTGCTGACATCGGCACCGGTCACCGTTCTGGAGGTGTCGGGGGAGCGGGTGGAAGACCTGGCCGCTACCGTGCTGGCGGCCGAGGCGGCCGGGGTGGCTCGATGGACGCTCGATACCGCGGTTGCCTACGCCAAGGTGCGGGAGCAATTCGGCAAGCCGATCGGCAGCTTCCAGGCTGTCAAGCACCTGTGTGCTCAGATGTTGTGCCGTGCCGAGCAGGCCGATGTGGCCGCCGCCGATGCGGCCCGCGCCGCAGCCGATTCCGATGGCACCCAACTTTCGATCGCCGCGGCGGTGGCCGCGAGCATCGGCATCGACGCCGCCAAAGCCAACGCCAAGGACTGTATCCAGGTGCTCGGTGGTATCGGCTGCACCTGGGAGCACGACGCGCATTTGTATCTGCGCCGGGCCCACGGAATCGGTGGGTTCCTCGGTGGATCCGGGCGCTGGCTGCGTCGGGTTACGGCGCTGACCCAGGCCGGTGTCCGTCGTCGCTTGGGCGTCGACCTGGCCGAGGTGGCCGGCCTGCGACCGGAGATCGCGGCGGCGGTCGCCGAGGTGGCCGCGTTGCCCGAGGAGAAACGTCAGGTGGCGCTGGCGGACACCGGCCTGCTGGCGCCGCACTGGCCGGCGCCGTACGGGCGCGGCGCGTCCCCGGCCGAGCAACTGCTGATCGATCAGGAACTGGCCGCGGCCAAGGTTGAGCGCCCGGACCTGGTGATCGGCTGGTGGGCGGCGCCGACCATCCTTGAACACGGCACGCCGGAACAGATCGAACGTTTCGTCCCGGCCACCATGCGCGGTGAATTCCTTTGGTGTCAGCTATTTTCCGAGCCGGGGGCCGGTTCGGACCTGGCGTCGCTGCGTACCAAGGCGGTGCGGGCTGACGGCGGCTGGCTGCTCACCGGACAGAAGGTGTGGACGTCGGCGGCTCACAAGGCTCGGTGGGGGGTGTGCCTGGCGCGCACCGACCCGGATGCTCCGAAGCACAAGGGCATTACCTACTTCCTGGTGGACATGACCACACCGGGAATTGAGATCCGACCGCTGCGCGAGATCACCGGCGACTCGCTGTTCAACGAGGTCTTCCTGGACAATGTGTTCGTCCCCGACGAGATGGTCGTCGGCGCCGTGAACGACGGCTGGCGGCTGGCGCGCACCACGCTAGCCAACGAGCGGGTCGCGATGGCCACCGGGACCGCGCTGGGCAACCCGATGGAAGAGCTGCTCAAGGTCTTGGGTGACATGGAACTCGATGTCGCGCAGCAAGATCGGCTGGGCAGGCTGATCCTGCTGGCTCAGGCCGGTGCGCTGTTGGACCGGCGGATCGCCGAGTTGGCCGTCGGCGGCCAGGATCCGGGGGCGCAATCCAGCGTCCGCAAACTGATCGGCGTGCGCTACCGACAGGCGCTGGCCGAATACCTGATGGAGGTGTCCGACGGGGGCGGGCTCGTCGAGAACCGCGCGGTGTACGACTTCCTCAACACCCGGTGCCTGACGATAGCCGGCGGTACCGAGCAGATCCTGTTGACGGTGGCCGCCGAACGGCTGCTGGGTCTGCCGCGTTG
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Involved in the second cycle of side chain dehydrogenation in the beta-oxidation of cholesterol catabolism. It contributes partly to the virulence by increasing the efficiency of beta-oxidation. Catalyzes the highly specific dehydrogenation of acyl-CoA ester side chains of 3-oxo-chol-4-en-24-oyl-CoA (3-OCO-CoA) to yield 3-oxochol-4,22-dien-24-oyl-CoA. {ECO:0000269|PubMed:26161441}.

Family

Acyl-CoA dehydrogenase family

GO

pathogenesis GO:0009405 biological_process
plasma membrane GO:0005886 cellular_component
flavin adenine dinucleotide binding GO:0050660 molecular_function
oxidoreductase activity, acting on the CH-CH group of donors GO:0016627 molecular_function
cholesterol catabolic process GO:0006707 biological_process

InterPro

UniProt: P96855
GenBank: Rv3573c
EnsemblBacteria: Rv3573c
Mycobrowser: Rv3573c

Summary

Name: Acyl-CoA dehydrogenase FadE34 (ACAD) (EC 1.3.99.-) (3-oxochol-4-en-24-oyl-CoA dehydrogenase)
Family: Acyl-CoA dehydrogenase family
Protein Sequence: MVATVTDEQSAARELVRGWARTAASGAAATAAVRDMEYGFEEGNADAWRPVFAGLAGLGLFGVAVPEDCGGAGGSIEDLCAMVDEAARALVPGPVATTAVATLVVSDPKLRSALASGERFAGVAIDGGVQVDPKTSTASGTVGRVLGGAPGGVVLLPADGNWLLVDTACDEVVVEPLRATDFSLPLARMVLTSAPVTVLEVSGERVEDLAATVLAAEAAGVARWTLDTAVAYAKVREQFGKPIGSFQAVKHLCAQMLCRAEQADVAAADAARAAADSDGTQLSIAAAVAASIGIDAAKANAKDCIQVLGGIGCTWEHDAHLYLRRAHGIGGFLGGSGRWLRRVTALTQAGVRRRLGVDLAEVAGLRPEIAAAVAEVAALPEEKRQVALADTGLLAPHWPAPYGRGASPAEQLLIDQELAAAKVERPDLVIGWWAAPTILEHGTPEQIERFVPATMRGEFLWCQLFSEPGAGSDLASLRTKAVRADGGWLLTGQKVWTSAAHKARWGVCLARTDPDAPKHKGITYFLVDMTTPGIEIRPLREITGDSLFNEVFLDNVFVPDEMVVGAVNDGWRLARTTLANERVAMATGTALGNPMEELLKVLGDMELDVAQQDRLGRLILLAQAGALLDRRIAELAVGGQDPGAQSSVRKLIGVRYRQALAEYLMEVSDGGGLVENRAVYDFLNTRCLTIAGGTEQILLTVAAERLLGLPR
Mass: 74,578 Da
Length: 711 Aa

Rv3573c doesn't seem to be a targeted by any drug.

Rv3573c doesn't seem to be involved in any pathway.

Unraveling Cholesterol Catabolism in Mycobacterium tuberculosis: ChsE4-ChsE5 alpha2beta2 Acyl-CoA Dehydrogenase Initiates beta-Oxidation of 3-Oxo-cholest-4-en-26-oyl CoA.: ACS Infect Dis. 2015 Feb 13;1(2):110-125. doi: 10.1021/id500033m. Epub 2015 Jan 8.
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
A highly conserved transcriptional repressor controls a large regulon involved in lipid degradation in Mycobacterium smegmatis and Mycobacterium tuberculosis.: Mol Microbiol. 2007 Aug;65(3):684-99. doi: 10.1111/j.1365-2958.2007.05827.x.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.