Rv3586
- Type
- protein_coding
- Name
- Rv3586
- Locus Name
-
Rv3586
- Product
-
Conserved hypothetical protein
- Functional Category
-
Conserved hypotheticals
- Location
- 4027891..4028967 (+ strand)
- Gene Length
- 1076 bp
- Nucleotides
-
TGCACGCTGTGACTCGTCCGACCCTGCGTGAGGCTGTCGCCCGCCTAGCCCCGGGCACTGGGCTGCGGGACGGCCTGGAGCGTATCCTGCGCGGCCGCACTGGTGCCCTGATCGTGCTGGGCCATGACGAGAATGTCGAGGCCATCTGCGATGGTGGCTTCTCCCTCGATGTCCGCTATGCAGCAACCCGGCTACGCGAGCTGTGCAAGATGGACGGCGCCGTGGTGCTGTCCACCGACGGCAGCCGCATCGTGCGGGCCAACGTGCAACTGGTACCGGATCCGTCGATCCCCACCGACGAATCGGGGACCCGGCACCGCTCGGCCGAGCGGGCCGCGATCCAGACCGGTTACCCGGTGATCTCAGTGAGCCACTCGATGAACATCGTGACCGTCTACGTCCGCGGGGAACGTCACGTATTGACCGACTCGGCAACCATCCTGTCGCGGGCCAACCAGGCCATCGCAACCCTGGAGCGGTACAAAACCAGGCTCGACGAGGTCAGCCGGCAACTGTCCAGGGCAGAAATCGAGGACTTCGTCACGCTGCGCGATGTGATGACGGTGGTGCAACGCCTCGAGCTGGTCCGGCGAATCGGGCTGGTGATCGACTACGACGTGGTCGAACTCGGCACTGATGGTCGTCAGCTGCGGCTGCAGCTCGACGAGTTGCTCGGCGGCAACGACACCGCCCGGGAATTGATCGTGCGCGATTACCACGCCAACCCGGAACCACCGTCCACGGGGCAAATCAATGCCACCCTGGACGAACTGGACGCCCTGTCGGACGGCGACCTCCTCGATTTCACCGCGCTGGCAAAGGTTTTCGGATATCCGACGACCACGGAAGCGCAGGATTCGACGCTGAGCCCGCGTGGCTACCGCGCGATGGCCGGTATCCCCCGGCTCCAGTTCGCCCATGCCGACCTGCTGGTCCGGGCGTTCGGAACGTTGCAGGGTCTGCTGGCGGCCAGCGCCGGCGATCTGCAATCAGTGGACGGCATCGGCGCCATGTGGGCCCGTCATGTGCGCGAGGGGTTGTCACAGCTGGCGGAATCGACCATCAGCGATCAATAA
- Drug Resistance
- Mutations
- Function
- Participates in a DNA-damage check-point. DisA forms globular foci that rapidly scan along the chromosomes searching for lesions. {ECO:0000255|HAMAP-Rule:MF_01438}.; FUNCTION: Has also diadenylate cyclase activity, catalyzing the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP). c-di-AMP likely acts as a signaling molecule that may couple DNA integrity with a cellular process. To a lesser extent, can also use ADP as substrate to produce c-di-AMP. Does not convert GTP to c-di-GMP. Also exhibits residual ATPase and ADPase activities in vitro. {ECO:0000255|HAMAP-Rule:MF_01438, ECO:0000269|PubMed:22529992}.
- Family
-
DisA family
- GO
-
- second-messenger-mediated signaling GO:0019932 biological_process
- ATP binding GO:0005524 molecular_function
- DNA binding GO:0003677 molecular_function
- DNA repair GO:0006281 biological_process
- nucleotidyltransferase activity GO:0016779 molecular_function
- adenylate cyclase activity GO:0004016 molecular_function
- InterPro
Summary
- Name
- DNA integrity scanning protein DisA (Cyclic-di-AMP synthase) (c-di-AMP synthase) (Diadenylate cyclase) (EC 2.7.7.85)
- Family
- DisA family
- Protein Sequence
-
MHAVTRPTLREAVARLAPGTGLRDGLERILRGRTGALIVLGHDENVEAICDGGFSLDVRYAATRLRELCKMDGAVVLSTDGSRIVRANVQLVPDPSIPTDESGTRHRSAERAAIQTGYPVISVSHSMNIVTVYVRGERHVLTDSATILSRANQAIATLERYKTRLDEVSRQLSRAEIEDFVTLRDVMTVVQRLELVRRIGLVIDYDVVELGTDGRQLRLQLDELLGGNDTARELIVRDYHANPEPPSTGQINATLDELDALSDGDLLDFTALAKVFGYPTTTEAQDSTLSPRGYRAMAGIPRLQFAHADLLVRAFGTLQGLLAASAGDLQSVDGIGAMWARHVREGLSQLAESTISDQ
- Mass
- 39,031 Da
- Length
- 358 Aa
Rv3586 doesn't seem to be a targeted by any drug.
Rv3586 doesn't seem to be involved in any pathway.
- Radiation-sensitive gene A (RadA) targets DisA, DNA integrity scanning protein A, to negatively affect cyclic Di-AMP synthesis activity in Mycobacterium smegmatis.
- J Biol Chem. 2013 Aug 2;288(31):22426-36. doi: 10.1074/jbc.M113.464883. Epub 2013 Jun 10.
- Mycobacterium tuberculosis Rv3586 (DacA) is a diadenylate cyclase that converts ATP or ADP into c-di-AMP.
- PLoS One. 2012;7(4):e35206. doi: 10.1371/journal.pone.0035206. Epub 2012 Apr 17.
- Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.
- Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
- Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.
- Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.