Gene - combatTB X

espA

Type: protein_coding
Name: espA
Locus Name: Rv3616c
Product: ESX-1 secretion-associated protein A, EspA
Functional Category: Cell wall and cell processes
Location: 4055197..4056375 (- strand)
Gene Length: 1178 bp
Nucleotides: ATGAGCAGAGCGTTCATCATCGATCCAACGATCAGTGCCATTGACGGCTTGTACGACCTTCTGGGGATTGGAATACCCAACCAAGGGGGTATCCTTTACTCCTCACTAGAGTACTTCGAAAAAGCCCTGGAGGAGCTGGCAGCAGCGTTTCCGGGTGATGGCTGGTTAGGTTCGGCCGCGGACAAATACGCCGGCAAAAACCGCAACCACGTGAATTTTTTCCAGGAACTGGCAGACCTCGATCGTCAGCTCATCAGCCTGATCCACGACCAGGCCAACGCGGTCCAGACGACCCGCGACATCCTGGAGGGCGCCAAGAAAGGTCTCGAGTTCGTGCGCCCGGTGGCTGTGGACCTGACCTACATCCCGGTCGTCGGGCACGCCCTATCGGCCGCCTTCCAGGCGCCGTTTTGCGCGGGCGCGATGGCCGTAGTGGGCGGCGCGCTTGCCTACTTGGTCGTGAAAACGCTGATCAACGCGACTCAACTCCTCAAATTGCTTGCCAAATTGGCGGAGTTGGTCGCGGCCGCCATTGCGGACATCATTTCGGATGTGGCGGACATCATCAAGGGCACCCTCGGAGAAGTGTGGGAGTTCATCACAAACGCGCTCAACGGCCTGAAAGAGCTTTGGGACAAGCTCACGGGGTGGGTGACCGGACTGTTCTCTCGAGGGTGGTCGAACCTGGAGTCCTTCTTTGCGGGCGTCCCCGGCTTGACCGGCGCGACCAGCGGCTTGTCGCAAGTGACTGGCTTGTTCGGTGCGGCCGGTCTGTCCGCATCGTCGGGCTTGGCTCACGCGGATAGCCTGGCGAGCTCAGCCAGCTTGCCCGCCCTGGCCGGCATTGGGGGCGGGTCCGGTTTTGGGGGCTTGCCGAGCCTGGCTCAGGTCCATGCCGCCTCAACTCGGCAGGCGCTACGGCCCCGAGCTGATGGCCCGGTCGGCGCCGCTGCCGAGCAGGTCGGCGGGCAGTCGCAGCTGGTCTCCGCGCAGGGTTCCCAAGGTATGGGCGGACCCGTAGGCATGGGCGGCATGCACCCCTCTTCGGGGGCGTCGAAAGGGACGACGACGAAGAAGTACTCGGAAGGCGCGGCGGCGGGCACTGAAGACGCCGAGCGCGCGCCAGTCGAAGCTGACGCGGGCGGTGGGCAAAAGGTGCTGGTACGAAACGTCGTCTA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Required for secretion of EsxA (ESAT-6) and EsxB (CFP-10) and for virulence. Involved in translocation of bacteria from the host (human) phagolysosome to the host cytoplasm (PubMed:17604718). {ECO:0000269|PubMed:16030141, ECO:0000269|PubMed:17604718, ECO:0000269|PubMed:24078612}.

Family

Unknown

GO

protein secretion by the type VII secretion system GO:0044315 biological_process
Actinobacterium-type cell wall biogenesis GO:0071766 biological_process
pathogenesis GO:0009405 biological_process
extracellular space GO:0005615 cellular_component
extracellular region GO:0005576 cellular_component
plasma membrane GO:0005886 cellular_component

InterPro

Unknown

UniProt: P9WJE1
GenBank: Rv3616c
EnsemblBacteria: Rv3616c
Mycobrowser: Rv3616c

Summary

Name: ESX-1 secretion-associated protein EspA
Family: Unknown
Protein Sequence: MSRAFIIDPTISAIDGLYDLLGIGIPNQGGILYSSLEYFEKALEELAAAFPGDGWLGSAADKYAGKNRNHVNFFQELADLDRQLISLIHDQANAVQTTRDILEGAKKGLEFVRPVAVDLTYIPVVGHALSAAFQAPFCAGAMAVVGGALAYLVVKTLINATQLLKLLAKLAELVAAAIADIISDVADIIKGTLGEVWEFITNALNGLKELWDKLTGWVTGLFSRGWSNLESFFAGVPGLTGATSGLSQVTGLFGAAGLSASSGLAHADSLASSASLPALAGIGGGSGFGGLPSLAQVHAASTRQALRPRADGPVGAAAEQVGGQSQLVSAQGSQGMGGPVGMGGMHPSSGASKGTTTKKYSEGAAAGTEDAERAPVEADAGGGQKVLVRNVV
Mass: 39,888 Da
Length: 392 Aa

Rv3616c doesn't seem to be a targeted by any drug.

Rv3616c doesn't seem to be involved in any pathway.

Mycobacterium tuberculosis Differentially Activates cGAS- and Inflammasome-Dependent Intracellular Immune Responses through ESX-1.: Cell Host Microbe. 2015 Jun 10;17(6):799-810. doi: 10.1016/j.chom.2015.05.003. Epub 2015 Jun 2.
EspR, a regulator of the ESX-1 secretion system in Mycobacterium tuberculosis, is directly regulated by the two-component systems MprAB and PhoPR.: Microbiology. 2015 Mar;161(Pt 3):477-89. doi: 10.1099/mic.0.000023. Epub 2014 Dec 23.
Phenotypic profiling of Mycobacterium tuberculosis EspA point mutants reveals that blockage of ESAT-6 and CFP-10 secretion in vitro does not always correlate with attenuation of virulence.: J Bacteriol. 2013 Dec;195(24):5421-30. doi: 10.1128/JB.00967-13. Epub 2013 Sep 27.
MprAB regulates the espA operon in Mycobacterium tuberculosis and modulates ESX-1 function and host cytokine response.: J Bacteriol. 2013 Jan;195(1):66-75. doi: 10.1128/JB.01067-12. Epub 2012 Oct 26.
Long-range transcriptional control of an operon necessary for virulence-critical ESX-1 secretion in Mycobacterium tuberculosis.: J Bacteriol. 2012 May;194(9):2307-20. doi: 10.1128/JB.00142-12. Epub 2012 Mar 2.
EspD is critical for the virulence-mediating ESX-1 secretion system in Mycobacterium tuberculosis.: J Bacteriol. 2012 Feb;194(4):884-93. doi: 10.1128/JB.06417-11. Epub 2011 Dec 9.
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
EspA acts as a critical mediator of ESX1-dependent virulence in Mycobacterium tuberculosis by affecting bacterial cell wall integrity.: PLoS Pathog. 2010 Jun 24;6(6):e1000957. doi: 10.1371/journal.ppat.1000957.
Conservation of structure and protein-protein interactions mediated by the secreted mycobacterial proteins EsxA, EsxB, and EspA.: J Bacteriol. 2010 Jan;192(1):326-35. doi: 10.1128/JB.01032-09.
Secreted transcription factor controls Mycobacterium tuberculosis virulence.: Nature. 2008 Aug 7;454(7205):717-21. doi: 10.1038/nature07219.
M. tuberculosis and M. leprae translocate from the phagolysosome to the cytosol in myeloid cells.: Cell. 2007 Jun 29;129(7):1287-98. doi: 10.1016/j.cell.2007.05.059.
The Mycobacterium tuberculosis PhoPR two-component system regulates genes essential for virulence and complex lipid biosynthesis.: Mol Microbiol. 2006 Apr;60(2):312-30. doi: 10.1111/j.1365-2958.2006.05102.x.
Mutually dependent secretion of proteins required for mycobacterial virulence.: Proc Natl Acad Sci U S A. 2005 Jul 26;102(30):10676-81. doi: 10.1073/pnas.0504922102. Epub 2005 Jul 19.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.