Gene - combatTB X

topA

Type: protein_coding
Name: topA
Locus Name: Rv3646c
Product: DNA topoisomerase I TopA (omega-protein) (relaxing enzyme) (untwisting enzyme) (swivelase) (type I DNA topoisomerase) (nicking-closing enzyme) (TOPO I)
Functional Category: Information pathways
Location: 4084453..4087257 (- strand)
Gene Length: 2804 bp
Nucleotides: TTGGCTGACCCGAAAACGAAGGGCCGTGGCAGCGGCGGCAATGGCAGCGGCCGGCGACTGGTCATCGTCGAGTCGCCCACCAAGGCGCGCAAGCTGGCCTCCTACCTGGGCTCTGGCTACATCGTCGAGTCCTCCCGGGGGCACATCCGTGACTTGCCGCGGGCCGCGTCGGATGTACCCGCAAAGTACAAGTCGCAGCCGTGGGCGCGGCTCGGGGTCAACGTCGACGCCGACTTCGAACCGCTCTACATCATCAGCCCGGAGAAACGGAGCACCGTCAGCGAGCTCAGGGGCCTGCTCAAAGACGTGGACGAGCTGTATCTGGCCACGGATGGGGACCGTGAGGGCGAAGCTATTGCCTGGCATCTGCTGGAAACCCTCAAACCGCGCATACCGGTAAAGCGGATGGTCTTCCACGAGATCACCGAACCGGCGATCCGCGCCGCCGCCGAGCACCCCCGCGACCTAGACATCGACCTGGTCGACGCGCAGGAGACCCGGCGCATCCTGGACCGGCTGTACGGCTACGAAGTCAGCCCAGTGCTGTGGAAGAAGGTCGCCCCCAAGTTGTCGGCGGGCCGGGTGCAGTCGGTGGCCACCCGCATCATCGTGGCGCGCGAACGCGACCGCATGGCGTTCCGCAGCGCGGCCTACTGGGACATCCTTGCCAAGCTGGATGCCAGCGTGTCCGACCCGGACGCCGCGCCGCCCACCTTCAGCGCCCGGCTGACGGCCGTGGCTGGCCGGCGGGTGGCCACTGGCCGCGATTTCGACTCGCTGGGCACGCTGCGCAAAGGCGACGAAGTCATTGTGCTCGACGAGGGGAGCGCGACCGCGTTGGCCGCGGGCCTGGATGGCACGCAGCTGACCGTGGCCTCGGCCGAGGAGAAGCCCTACGCCCGGCGCCCGTACCCGCCGTTCATGACCTCCACGCTGCAGCAAGAGGCCAGCCGCAAGCTGCGGTTCTCCGCCGAGCGGACGATGAGCATCGCCCAGCGGCTGTACGAAAACGGCTACATCACCTATATGCGTACCGACTCCACCACGCTGTCGGAGTCGGCGATCAACGCCGCACGTACCCAGGCGCGCCAGCTCTACGGCGACGAGTACGTCGCGCCGGCGCCGCGCCAATACACCCGCAAGGTGAAGAACGCCCAGGAAGCGCACGAGGCTATCCGGCCCGCCGGTGAAACGTTTGCCACCCCGGACGCGGTGCGTCGCGAACTCGACGGTCCCAACATTGATGATTTCCGGCTCTATGAGCTGATTTGGCAACGCACCGTAGCCTCGCAGATGGCCGATGCGCGGGGCATGACGCTGAGCCTGCGGATCACTGGCATGTCGGGGCACCAGGAGGTGGTGTTCTCCGCGACCGGACGCACCTTGACGTTCCCGGGCTTCCTCAAGGCCTACGTGGAGACCGTGGACGAGCTGGTCGGCGGCGAGGCTGACGATGCCGAGCGGCGACTGCCCCATCTGACCCCGGGTCAACGGTTGGACATCGTCGAGTTGACCCCAGACGGCCATGCCACCAACCCGCCGGCCCGCTACACCGAGGCGTCGCTGGTCAAAGCGCTCGAGGAGCTGGGCATCGGCCGCCCGTCGACCTACTCGTCGATCATCAAGACCATCCAGGATCGCGGCTACGTGCACAAGAAGGGCAGTGCACTGGTGCCGTCATGGGTGGCGTTCGCGGTAACCGGTCTGCTCGAGCAGCATTTCGGTCGGCTCGTCGACTACGACTTCACCGCGGCGATGGAAGACGAGCTCGACGAGATCGCCGCCGGCAACGAGCGCCGCACCAACTGGCTCAACAACTTCTACTTTGGTGGCGATCACGGTGTGCCCGATTCGGTAGCCCGATCGGGTGGCCTCAAGAAGCTTGTCGGGATCAATCTCGAGGGCATCGACGCACGAGAAGTAAACTCTATCAAGCTTTTTGACGACACCCACGGACGCCCCATATATGTTCGGGTGGGCAAGAACGGTCCCTACCTGGAACGTTTGGTGGCCGGCGACACCGGTGAGCCCACGCCGCAGCGGGCCAACCTCAGCGACTCGATTACCCCGGACGAGCTGACTCTACAGGTGGCCGAAGAGCTCTTTGCCACACCGCAACAGGGACGGACTTTGGGCTTGGACCCAGAAACCGGCCACGAGATCGTGGCCAGGGAAGGCCGGTTTGGGCCGTATGTGACCGAGATCCTGCCGGAGCCTGCGGCTGATGCGGCCGCGGCCGCTCAGGGAGTCAAGAAACGCCAGAAGGCCGCCGGGCCCAAACCGCGCACCGGTTCGTTGCTGCGGAGCATGGACCTACAGACGGTCACCCTCGAAGACGCGCTGAGGCTGCTGTCACTGCCGCGCGTGGTCGGAGTGGACCCCGCCTCGGGTGAGGAGATCACCGCGCAGAACGGGCGCTACGGACCGTATCTAAAGCGCGGCAACGATTCTCGATCACTGGTCACCGAAGACCAGATATTCACCATCACGCTCGACGAAGCCCTGAAGATCTACGCAGAGCCGAAACGTCGTGGCCGGCAAAGCGCTTCGGCTCCGCCGCTGCGCGAGCTGGGAACAGATCCGGCGTCGGGCAAGCCAATGGTCATCAAGGACGGCCGATTCGGGCCGTACGTCACCGACGGTGAGACCAATGCCAGCCTGCGTAAGGGCGACGACGTGGCTTCCATAACCGACGAGCGCGCCGCCGAGCTGTTGGCCGATCGCCGAGCCCGGGGTCCGGCAAAACGGCCAGCCAGGAAAGCTGCCCGGAAGGTGCCGGCGAAGAAGGCAGCCAAGCGCGACTA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex (PubMed:8921893, PubMed:20724443). Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. {ECO:0000255|HAMAP-Rule:MF_00952, ECO:0000269|PubMed:20724443, ECO:0000269|PubMed:8921893}.; FUNCTION: The C-terminus (residues 622-934) inhibits RNA cleavage by MazF4. {ECO:0000269|PubMed:20724443}.

Family

Type IA topoisomerase family

GO

magnesium ion binding GO:0000287 molecular_function
DNA topoisomerase type I activity GO:0003917 molecular_function
cell wall GO:0005618 cellular_component
cytosol GO:0005829 cellular_component
DNA binding GO:0003677 molecular_function
DNA topological change GO:0006265 biological_process
plasma membrane GO:0005886 cellular_component
negative regulation of ribonuclease activity GO:0060701 biological_process

InterPro

UniProt: P9WG49
GenBank: Rv3646c
EnsemblBacteria: Rv3646c
Mycobrowser: Rv3646c

Summary

Name: DNA topoisomerase 1 (EC 5.6.2.2) (DNA topoisomerase I) (Omega-protein) (Relaxing enzyme) (Swivelase) (Untwisting enzyme)
Family: Type IA topoisomerase family
Protein Sequence: MADPKTKGRGSGGNGSGRRLVIVESPTKARKLASYLGSGYIVESSRGHIRDLPRAASDVPAKYKSQPWARLGVNVDADFEPLYIISPEKRSTVSELRGLLKDVDELYLATDGDREGEAIAWHLLETLKPRIPVKRMVFHEITEPAIRAAAEHPRDLDIDLVDAQETRRILDRLYGYEVSPVLWKKVAPKLSAGRVQSVATRIIVARERDRMAFRSAAYWDILAKLDASVSDPDAAPPTFSARLTAVAGRRVATGRDFDSLGTLRKGDEVIVLDEGSATALAAGLDGTQLTVASAEEKPYARRPYPPFMTSTLQQEASRKLRFSAERTMSIAQRLYENGYITYMRTDSTTLSESAINAARTQARQLYGDEYVAPAPRQYTRKVKNAQEAHEAIRPAGETFATPDAVRRELDGPNIDDFRLYELIWQRTVASQMADARGMTLSLRITGMSGHQEVVFSATGRTLTFPGFLKAYVETVDELVGGEADDAERRLPHLTPGQRLDIVELTPDGHATNPPARYTEASLVKALEELGIGRPSTYSSIIKTIQDRGYVHKKGSALVPSWVAFAVTGLLEQHFGRLVDYDFTAAMEDELDEIAAGNERRTNWLNNFYFGGDHGVPDSVARSGGLKKLVGINLEGIDAREVNSIKLFDDTHGRPIYVRVGKNGPYLERLVAGDTGEPTPQRANLSDSITPDELTLQVAEELFATPQQGRTLGLDPETGHEIVAREGRFGPYVTEILPEPAADAAAAAQGVKKRQKAAGPKPRTGSLLRSMDLQTVTLEDALRLLSLPRVVGVDPASGEEITAQNGRYGPYLKRGNDSRSLVTEDQIFTITLDEALKIYAEPKRRGRQSASAPPLRELGTDPASGKPMVIKDGRFGPYVTDGETNASLRKGDDVASITDERAAELLADRRARGPAKRPARKAARKVPAKKAAKRD
Mass: 102,336 Da
Length: 934 Aa

Rv3646c doesn't seem to be a targeted by any drug.

Rv3646c doesn't seem to be involved in any pathway.

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Characterization of an interplay between a Mycobacterium tuberculosis MazF homolog, Rv1495 and its sole DNA topoisomerase I.: Nucleic Acids Res. 2010 Dec;38(22):8219-30. doi: 10.1093/nar/gkq737. Epub 2010 Aug 19.
targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis.: BMC Syst Biol. 2008 Dec 19;2:109. doi: 10.1186/1752-0509-2-109.
Cloning, expression, purification and characterization of DNA topoisomerase I of Mycobacterium tuberculosis.: Gene. 1996 Oct 31;178(1-2):63-9.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.