Gene - combatTB X

asd

Type: protein_coding
Name: asd
Locus Name: Rv3708c
Product: Aspartate-semialdehyde dehydrogenase Asd (ASA dehydrogenase) (ASADH) (aspartic semialdehyde dehydrogenase) (L-aspartate-beta-semialdehyde dehydrogenase)
Functional Category: Intermediary metabolism and respiration
Location: 4151180..4152217 (- strand)
Gene Length: 1037 bp
Nucleotides: ATGGGCCTGTCAATAGGGATCGTGGGGGCCACCGGTCAGGTGGGTCAGGTCATGCGCACGTTGCTCGACGAGCGGGATTTCCCGGCGAGCGCGGTGCGGTTCTTCGCGTCGGCCCGATCGCAGGGCCGCAAGCTGGCCTTCCGCGGCCAGGAGATCGAAGTGGAAGACGCCGAGACGGCCGACCCGAGCGGGCTGGATATCGCGTTGTTCTCCGCCGGCTCGGCCATGTCGAAGGTGCAGGCGCCCCGCTTTGCGGCGGCCGGAGTCACGGTGATCGACAACTCGTCGGCGTGGCGTAAGGACCCCGACGTGCCGTTGGTGGTGTCCGAGGTGAACTTTGAACGCGACGCGCACCGCCGGCCTAAGGGCATCATCGCCAACCCGAACTGCACCACCATGGCCGCGATGCCGGTGCTCAAGGTGCTGCACGACGAAGCCCGACTGGTGCGGTTGGTGGTCTCGTCGTATCAGGCGGTGTCCGGTAGCGGCCTGGCCGGGGTGGCCGAGCTGGCCGAGCAGGCGCGTGCGGTCATCGGTGGGGCCGAGCAGCTGGTGTATGACGGCGGCGCGCTCGAGTTCCCGCCGCCGAACACCTACGTCGCCCCGATCGCGTTCAACGTGGTGCCGCTGGCCGGATCCCTGGTGGACGACGGCTCCGGCGAGACAGACGAGGATCAAAAGTTGCGCTTCGAGAGCCGCAAGATCCTTGGCATCCCCGACCTGTTGGTCAGCGGCACCTGTGTGCGGGTTCCGGTGTTCACCGGACACTCTTTGTCGATCAACGCCGAGTTCGCGCAGCCGCTCTCGCCGGAGCGGGCGCGCGAGCTGCTCGACGGTGCTACGGGCGTGCAGCTGGTCGACGTGCCGACGCCGCTGGCCGCCGCCGGTGTCGACGAATCTCTGGTCGGCCGGATCCGGCGCGACCCGGGGGTGCCCGACGGGCGTGGTCTGGCGCTGTTCGTGTCGGGGGATAACCTGCGCAAGGGGGCGGCGCTGAACACCATCCAGATCGCCGAGCTGCTGACCGCCGACTTGTG
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Catalyzes the NADPH-dependent formation of L-aspartate-semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate (PubMed:15752328). Is essential for the growth and pathogenicity of M.tuberculosis, and for the generation of the bacterial cell wall (PubMed:26437401). {ECO:0000269|PubMed:15752328, ECO:0000269|PubMed:26437401}.

Family

Aspartate-semialdehyde dehydrogenase family

GO

protein dimerization activity GO:0046983 molecular_function
isoleucine biosynthetic process GO:0009097 biological_process
pathogenesis GO:0009405 biological_process
'de novo' L-methionine biosynthetic process GO:0071266 biological_process
threonine biosynthetic process GO:0009088 biological_process
aspartate-semialdehyde dehydrogenase activity GO:0004073 molecular_function
NAD binding GO:0051287 molecular_function
NADP binding GO:0050661 molecular_function
plasma membrane GO:0005886 cellular_component
cell wall GO:0005618 cellular_component
diaminopimelate biosynthetic process GO:0019877 biological_process
lysine biosynthetic process via diaminopimelate GO:0009089 biological_process

InterPro

UniProt: P9WNX5
GenBank: Rv3708c
EnsemblBacteria: Rv3708c
Mycobrowser: Rv3708c

Summary

Name: Aspartate-semialdehyde dehydrogenase (ASA dehydrogenase) (ASADH) (EC 1.2.1.11) (Aspartate-beta-semialdehyde dehydrogenase)
Family: Aspartate-semialdehyde dehydrogenase family
Protein Sequence: MGLSIGIVGATGQVGQVMRTLLDERDFPASAVRFFASARSQGRKLAFRGQEIEVEDAETADPSGLDIALFSAGSAMSKVQAPRFAAAGVTVIDNSSAWRKDPDVPLVVSEVNFERDAHRRPKGIIANPNCTTMAAMPVLKVLHDEARLVRLVVSSYQAVSGSGLAGVAELAEQARAVIGGAEQLVYDGGALEFPPPNTYVAPIAFNVVPLAGSLVDDGSGETDEDQKLRFESRKILGIPDLLVSGTCVRVPVFTGHSLSINAEFAQPLSPERARELLDGATGVQLVDVPTPLAAAGVDESLVGRIRRDPGVPDGRGLALFVSGDNLRKGAALNTIQIAELLTADL
Mass: 36,230 Da
Length: 345 Aa

Rv3708c doesn't seem to be a targeted by any drug.

Cysteine and methionine metabolism mtu00270
mtu00270
Metabolism; Amino acid metabolism

Monobactam biosynthesis mtu00261
mtu00261
Metabolism; Biosynthesis of other secondary metabolites

Glycine, serine and threonine metabolism mtu00260
mtu00260
Metabolism; Amino acid metabolism

Structures of ternary complexes of aspartate-semialdehyde dehydrogenase (Rv3708c) from Mycobacterium tuberculosis H37Rv.: Acta Crystallogr D Biol Crystallogr. 2012 Jun;68(Pt 6):671-9. doi: 10.1107/S0907444912007330. Epub 2012 May 17.
Shape-based virtual screening, docking, and molecular dynamics simulations to identify Mtb-ASADH inhibitors.: J Biomol Struct Dyn. 2015;33(5):1082-93. doi: 10.1080/07391102.2014.929535. Epub 2014 Jun 23.
Pharmacoinformatics analysis to identify inhibitors of Mtb-ASADH.: J Biomol Struct Dyn. 2016;34(1):1-14. doi: 10.1080/07391102.2015.1005137. Epub 2015 Feb 11.
Identification and Validation of Aspartic Acid Semialdehyde Dehydrogenase as a New Anti-Mycobacterium Tuberculosis Target.: Int J Mol Sci. 2015 Sep 30;16(10):23572-86. doi: 10.3390/ijms161023572.
Molecular modelling and comparative structural account of aspartyl beta-semialdehyde dehydrogenase of Mycobacterium tuberculosis (H37Rv).: J Mol Model. 2008 Apr;14(4):249-63. doi: 10.1007/s00894-008-0267-2. Epub 2008 Jan 31.
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Purification, crystallization and preliminary X-ray diffraction analysis of aspartate semialdehyde dehydrogenase (Rv3708c) from Mycobacterium tuberculosis.: Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Mar 1;64(Pt 3):167-70. doi: 10.1107/S1744309108002753. Epub 2008 Feb 23.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.
Cloning and characterization of aspartate-beta-semialdehyde dehydrogenase from Mycobacterium tuberculosis H37 Rv.: J Appl Microbiol. 2005;98(4):832-8. doi: 10.1111/j.1365-2672.2004.02505.x.