Gene - combatTB X

tyrA

Type: protein_coding
Name: tyrA
Locus Name: Rv3754
Product: Prephenate dehydrogenase TyrA (PDH) (hydroxyphenylpyruvate synthase)
Functional Category: None
Location: 4200421..4201326 (+ strand)
Gene Length: 905 bp
Nucleotides: TGCGGGCCGCCGCAGCGGCGGGCCGTGAAGTCTTTGGCTACAACCGGTCGGTGGAGGGTGCCCACGGCGCCCGCTCCGACGGGTTTGATGCCATAACCGATCTCAACCAAACGCTAACCCGGGCCGCCGCTACCGAGGCGTTGATCGTGCTGGCCGTTCCGATGCCGGCCTTGCCAGGCATGCTCGCCCATATTCGCAAATCGGCACCTGGCTGTCCGTTGACCGACGTCACCAGCGTCAAATGCGCGGTTCTCGACGAGGTCACGGCGGCTGGTCTGCAGGCGCGCTACGTCGGCGGTCACCCGATGACGGGCACCGCGCACTCGGGTTGGACCGCCGGTCACGGCGGCTTGTTCAACAGAGCCCCCTGGGTGGTCAGCGTCGATGACCATGTCGACCCCACGGTGTGGTCGATGGTGATGACGCTGGCGCTGGACTGCGGGGCGATGGTGGTGCCCGCCAAATCCGACGAGCACGACGCCGCCGCTGCTGCCGTCTCGCACCTGCCACACCTGCTCGCTGAGGCGCTCGCCGTCACTGCGGCCGAGGTACCACTTGCCTTCGCGTTGGCTGCAGGGTCTTTCCGCGATGCCACCCGGGTGGCAGCCACCGCTCCTGACCTAGTGCGGGCAATGTGTGAAGCTAACACCGGCCAACTGGCGCCGGCCGCGGACCGGATCATCGACCTGCTGAGCCGTGCGCGTGATTCGCTGCAATCCCACGGTTCGATAGCCGACCTCGCCGACGCGGGCCACGCCGCACGCACACGCTATGACAGCTTCCCGCGCTCCGACATCGTCACCGTCGTTATTGGCGCGGACAAATGGCGCGAGCAACTGGCCGCCGCGGGGCGGGCGGGCGGGGTGATTACATCCGCTCTGCCAAGCCTGGATAGTCCACAATGA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Catalyzes the NAD(+)-dependent conversion of prephenate to p-hydroxyphenylpyruvate, with the elimination of carbon dioxide. Is a key regulatory enzyme in tyrosine biosynthesis. Displays no chorismate mutase (CM) activity, in contrast to TyrA from E.coli and some other bacteria, that are bifunctional and possess a CM domain. {ECO:0000269|PubMed:16889979}.

Family

Prephenate/arogenate dehydrogenase family

GO

prephenate dehydrogenase (NAD+) activity GO:0008977 molecular_function
NAD+ binding GO:0070403 molecular_function
oxidation-reduction process GO:0055114 biological_process
tyrosine biosynthetic process GO:0006571 biological_process
prephenate dehydrogenase (NADP+) activity GO:0004665 molecular_function

InterPro

UniProt: O69721
GenBank: Rv3754
EnsemblBacteria: Rv3754
Mycobrowser: Rv3754

Summary

Name: Prephenate dehydrogenase (PDH) (EC 1.3.1.12)
Family: Prephenate/arogenate dehydrogenase family
Protein Sequence: MTRTVAAPPVCVLGLGLIGGSIMRAAAAAGREVFGYNRSVEGAHGARSDGFDAITDLNQTLTRAAATEALIVLAVPMPALPGMLAHIRKSAPGCPLTDVTSVKCAVLDEVTAAGLQARYVGGHPMTGTAHSGWTAGHGGLFNRAPWVVSVDDHVDPTVWSMVMTLALDCGAMVVPAKSDEHDAAAAAVSHLPHLLAEALAVTAAEVPLAFALAAGSFRDATRVAATAPDLVRAMCEANTGQLAPAADRIIDLLSRARDSLQSHGSIADLADAGHAARTRYDSFPRSDIVTVVIGADKWREQLAAAGRAGGVITSALPSLDSPQ
Mass: 33,099 Da
Length: 323 Aa

Rv3754 doesn't seem to be a targeted by any drug.

Phenylalanine, tyrosine and tryptophan biosynthesis mtu00400
mtu00400
PATHWAY_MAP mtu00400 Phenylalanine, tyrosine and tryptophan biosynthesis

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Purification and characterization of a functionally active Mycobacterium tuberculosis prephenate dehydrogenase.: Protein Expr Purif. 2006 Oct;49(2):151-8. doi: 10.1016/j.pep.2006.05.020. Epub 2006 Jun 16.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.