Gene - combatTB X

aftB

Type: protein_coding
Name: aftB
Locus Name: Rv3805c
Product: Possible arabinofuranosyltransferase AftB
Functional Category: None
Location: 4266953..4268836 (- strand)
Gene Length: 1883 bp
Nucleotides: ATGGTCCGGGTCAGCTTGTGGCTGTCGGTGACGGCGGTCGCCGTGCTGTTCGGCTGGGGGAGCTGGCAACGTCGCTGGATCGCTGACGACGGACTGATCGTCCTGCGGACGGTACGCAACCTATTGGCCGGCAACGGACCGGTCTTCAACCAGGGCGAGCGAGTCGAAGCGAACACCTCGACAGCGTGGACGTATCTGCTGTATGTGGGCGGCTGGGTGGGCGGGCCGATGCGCCTGGAGTACGTGGCGTTGGCCCTGGCCATGGTGCTTTCGCTGCTGGGAATGGTGCTGCTCATGCTGGGGACCGGCCGGTTGTACGCACCCAGCCTGCGGGGCCGTCGGGCGATCATGCTTCCGGCCGGGGCGCTGGTATATATCGCGGTGCCACCGGCCCGCGACTTCGCCACCTCCGGCCTGGAGAGCGGGCTGGTGTTGGCCTATCTGGGGCTGTTGTGGTGGATGATGGTCTGCTGGTCACAGCCGCTGCGGGCCCGCCCGGACAGCCAGATGTTCCTCGGCGCGTTGGCCTTCGTCGCCGGATGCAGCGTGCTAGTGCGGCCGGAATTCGCGCTGATCGGCGGGCTGGCGCTGATCATGATGTTGATCGCCGCGCGGACCTGGCGTCGCCGCGTGCTGATCGTGCTGGCCGGCGGATTCTTGCCGGTGGCCTACCAGATCTTCCGGATGGGCTATTACGGACTGCTGGTTCCGAGTACCGCGCTGGCCAAGGATGCCGCGGGCGACAAGTGGTCGCAAGGCATGATCTACGTGTCGAACTTCAACCGGCCGTACGCACTGTGGGTGCCGTTGGTGCTGTCGGTGCCGCTGGGATTGCTGTTGATGACGGCGCGCCGCCGGCCGTCGTTCCTGCGTCCCGTGCTGGCACCCGACTACGGTCGGGTGGCCCGGGCGGTGCAAAGTCCGCCGGCCGTGGTGGCTTTCATCGTCGGCAGCGGCGTGCTGCAGGCGCTGTACTGGATTCGGCAAGGCGGCGACTTTATGCACGGCCGGGTGTTGCTGGCGCCGCTGTTTTGTTTGCTGGCCCCGGTGGGGGTCATTCCTATTTTGCTGCCCGACGGTAAGGACTTTTCGCGGGAGACCGGTCGCTGGCTAGTCGGCGCGCTCAGCGGGCTCTGGCTGGGGATCGCGGGTTGGTCGCTGTGGGCGGCAAACTCGCCGGGCATGGGTGACGACGCCACCCGGGTCACCTATTCGGGCATCGTCGACGAGCGCCGCTTCTACGCCCAGGCCACCGGGCACGCGCATCCGCTGACCGCCGCGGACTACCTCGATTACCCGCGAATGGCTGCCGTGCTTACAGCGCTCAACAACACCCCGGAAGGGGCGTTGCTATTGCCGTCGGGCAACTACAACCAGTGGGACCTGGTGCCGATGATCCGGCCCTCGTCGGGGACCGCGCCGGGTGGCAAGCCGGCGCCGAAACCGCAGCACGCAGTGTTTTTCACCAACATGGGCATGCTAGGCATGAACGTCGGGCTCGATGTCCGGGTGATCGACCAAATCGGGCTGGTAAACCCATTGGCCGCGCACACCGAGCGGCTGAAGCATGCCCGTATCGGCCATGACAAGAATCTGTTCCCGGACTGGGTGATCGCCGACGGACCCTGGGTGAAGTGGTACCCGGGTATTCCCGGCTACATCGACCAGCAGTGGGTCACGCAAGCCGAAGCGGCCCTGCAATGCCCCGCGACTCGGGCGGTGCTTAATTCTGTGCGCGCCCCGATCACCCTGCACCGATTCCTCTCCAACGTGTTGCATTCCTACGAGTTCACCCGTTACCGCATCGACCGCGTTCCGCGCTATGAATTGGTCCGGTGTGGGCTCGATGTTCCCGACGGGCCCGGCCCGCCACCGCGGGAGTG
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Involved in the biosynthesis of the arabinogalactan (AG) region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an essential component of the mycobacterial cell wall. Catalyzes the transfer of arabinofuranosyl (Araf) residues from the sugar donor decaprenyl-phospho-arabinose (DPA) to the arabinan domain to form terminal beta-(1->2)-linked Araf residues, which marks the end point for AG arabinan biosynthesis before decoration with mycolic acids. {ECO:0000269|PubMed:17387176}.

Family

AftB family

GO

cell wall organization GO:0071555 biological_process
capsule polysaccharide biosynthetic process GO:0045227 biological_process
transferase activity, transferring glycosyl groups GO:0016757 molecular_function
integral component of membrane GO:0016021 cellular_component

InterPro

Unknown

UniProt: O53582
GenBank: Rv3805c
EnsemblBacteria: Rv3805c
Mycobrowser: Rv3805c

Summary

Name: Terminal beta-(1->2)-arabinofuranosyltransferase (EC 2.4.2.-)
Family: AftB family
Protein Sequence: MVRVSLWLSVTAVAVLFGWGSWQRRWIADDGLIVLRTVRNLLAGNGPVFNQGERVEANTSTAWTYLLYVGGWVGGPMRLEYVALALAMVLSLLGMVLLMLGTGRLYAPSLRGRRAIMLPAGALVYIAVPPARDFATSGLESGLVLAYLGLLWWMMVCWSQPLRARPDSQMFLGALAFVAGCSVLVRPEFALIGGLALIMMLIAARTWRRRVLIVLAGGFLPVAYQIFRMGYYGLLVPSTALAKDAAGDKWSQGMIYVSNFNRPYALWVPLVLSVPLGLLLMTARRRPSFLRPVLAPDYGRVARAVQSPPAVVAFIVGSGVLQALYWIRQGGDFMHGRVLLAPLFCLLAPVGVIPILLPDGKDFSRETGRWLVGALSGLWLGIAGWSLWAANSPGMGDDATRVTYSGIVDERRFYAQATGHAHPLTAADYLDYPRMAAVLTALNNTPEGALLLPSGNYNQWDLVPMIRPSSGTAPGGKPAPKPQHAVFFTNMGMLGMNVGLDVRVIDQIGLVNPLAAHTERLKHARIGHDKNLFPDWVIADGPWVKWYPGIPGYIDQQWVTQAEAALQCPATRAVLNSVRAPITLHRFLSNVLHSYEFTRYRIDRVPRYELVRCGLDVPDGPGPPPRE
Mass: 68,710 Da
Length: 627 Aa

Rv3805c doesn't seem to be a targeted by any drug.

Arabinogalactan biosynthesis - Mycobacterium mtu00572
mtu00572
Metabolism; Glycan biosynthesis and metabolism

Lipoarabinomannan (LAM) biosynthesis mtu00571
mtu00571
Metabolism; Glycan biosynthesis and metabolism

Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Identification of a novel arabinofuranosyltransferase AftB involved in a terminal step of cell wall arabinan biosynthesis in Corynebacterianeae, such as Corynebacterium glutamicum and Mycobacterium tuberculosis.: J Biol Chem. 2007 May 18;282(20):14729-40. doi: 10.1074/jbc.M700271200. Epub 2007 Mar 26.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.