Gene - combatTB X

glfT2

Type: protein_coding
Name: glfT2
Locus Name: Rv3808c
Product: Bifunctional UDP-galactofuranosyl transferase GlfT2
Functional Category: None
Location: 4270366..4272279 (- strand)
Gene Length: 1913 bp
Nucleotides: ATGAGTGAACTCGCCGCGAGCCTGCTGTCTCGGGTCATCCTGCCGCGCCCAGGTGAACCCCTTGACGTGCGCAAGCTGTACCTCGAGGAGTCAACGACCAACGCCCGGCGCGCGCATGCGCCAACCCGGACCTCGCTACAGATTGGCGCGGAGTCCGAGGTGTCGTTCGCCACCTACTTCAACGCTTTCCCGGCTAGCTATTGGCGACGTTGGACGACGTGCAAATCGGTGGTGTTGCGCGTGCAGGTGACCGGCGCCGGGCGGGTAGACGTCTACCGGACCAAGGCCACCGGTGCCCGGATCTTCGTTGAGGGCCACGACTTCACCGGCACCGAGGATCAGCCAGCCGCCGTGGAGACCGAAGTGGTGCTGCAGCCTTTCGAAGACGGCGGGTGGGTCTGGTTCGACATCACCACCGACACCGCGGTCACCCTGCACAGTGGTGGCTGGTATGCCACCAGCCCCGCTCCGGGGACGGCCAACATCGCGGTCGGCATCCCGACGTTCAACCGCCCCGCAGACTGCGTCAACGCGCTGCGAGAACTCACCGCCGATCCGCTGGTGGACCAGGTGATCGGGGCGGTGATCGTGCCTGATCAGGGTGAGCGCAAGGTGCGCGACCACCCGGATTTCCCCGCCGCGGCTGCGAGATTGGGTAGTCGGCTCTCCATCCACGACCAGCCCAACCTGGGCGGTTCCGGCGGCTACAGCCGGGTGATGTATGAGGCACTGAAAAACACCGACTGTCAGCAGATCCTATTCATGGACGACGACATCCGCCTCGAGCCGGACTCGATTCTGCGGGTGCTGGCCATGCACCGGTTCGCCAAGGCGCCGATGCTGGTGGGTGGTCAGATGCTCAACCTGCAGGAGCCGTCGCACCTGCACATCATGGGCGAAGTCGTAGACCGGTCGATATTCATGTGGACCGCCGCGCCGCATGCCGAGTATGACCACGACTTCGCCGAATACCCGTTGAACGACAACAACTCCCGTAGCAAGTTGCTGCACCGGCGTATCGATGTCGACTACAACGGCTGGTGGACGTGCATGATCCCACGCCAGGTCGCCGAAGAGCTAGGACAGCCGCTGCCGTTGTTCATCAAATGGGACGACGCCGATTACGGCCTGCGGGCCGCCGAGCACGGGTATCCGACCGTCACGCTGCCCGGTGCGGCGATCTGGCACATGGCCTGGAGCGATAAGGATGACGCCATCGACTGGCAGGCCTATTTCCATCTGCGCAACCGGCTGGTGGTGGCCGCCATGCACTGGGACGGCCCAAAGGCGCAAGTCATCGGCCTGGTCCGCAGCCATCTCAAGGCAACCCTGAAACACCTTGCCTGCCTGGAATATTCGACGGTGGCAATCCAGAACAAGGCCATCGACGACTTTCTCGCCGGCCCCGAGCACATCTTTTCGATCCTGGAATCGGCGCTGCCGCAAGTGCACCGCATCCGCAAGAGCTATCCGGACGCGGTGGTGCTGCCGGCGGCCAGTGAGTTGCCGCCGCCGTTGCACAAGAACAAGGCAATGAAGCCGCCGGTGAACCCGTTGGTCATCGGCTACCGGCTGGCCCGCGGGATCATGCACAACCTGACCGCCGCCAACCCCCAACACCACCGTCGCCCGGAGTTCAACGTTCCGACCCAGGATGCGCGCTGGTTCCTGCTGTGCACTGTCGACGGGGCCACCGTCACCACGGCCGACGGATGCGGCGTGGTCTACCGGCAGCGCGACCGGGCCAAGATGTTCGCGTTGCTGTGGCAGTCGTTGCGCCGTCAGCGCCAGTTGCTGAAACGGTTCGAGGAGATGCGCCGGATTTATCGCGACGCGTTGCCGACGCTGTCCAGCAAGCAGAAGTGGGAGACGGCGCTGCTGCCGGCGGCAAACCAAGAGCCCGAGCATGGCTG
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Involved in the galactan polymerization of the arabinogalactan (AG) region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an essential component of the mycobacteria cell wall. Thus, successively transfers approximately 28 galactofuranosyl (Galf) residues from UDP-galactofuranose (UDP-Galf) onto the galactofuranosyl-galactofuranosyl-rhamnosyl-GlcNAc-diphospho-decaprenol (Galf-Galf-Rha-GlcNAc-PP-C50) acceptor produced by GlfT1, with alternating 1->5 and 1->6 links, forming a galactan domain with approximately 30 galactofuranosyl residues. {ECO:0000269|PubMed:18055597, ECO:0000269|PubMed:19571009, ECO:0000305|PubMed:10934214, ECO:0000305|PubMed:11304545, ECO:0000305|PubMed:16704275, ECO:0000305|PubMed:18423586}.

Family

Glycosyltransferase 2 family

GO

transferase activity, transferring glycosyl groups GO:0016757 molecular_function
transferase activity GO:0016740 molecular_function
capsule polysaccharide biosynthetic process GO:0045227 biological_process
lipopolysaccharide-1,5-galactosyltransferase activity GO:0035496 molecular_function
UDP-galactosyltransferase activity GO:0035250 molecular_function
plasma membrane GO:0005886 cellular_component
lipopolysaccharide-1,6-galactosyltransferase activity GO:0008921 molecular_function
UDP-D-galactose metabolic process GO:0052573 biological_process
cytosol GO:0005829 cellular_component
cell wall polysaccharide biosynthetic process GO:0070592 biological_process
lipopolysaccharide biosynthetic process GO:0009103 biological_process
cell wall macromolecule biosynthetic process GO:0044038 biological_process
mycolate cell wall layer assembly GO:0071769 biological_process
cell wall organization GO:0071555 biological_process
metal ion binding GO:0046872 molecular_function

InterPro

UniProt: O53585
GenBank: Rv3808c
EnsemblBacteria: Rv3808c
Mycobrowser: Rv3808c

Summary

Name: Galactofuranosyltransferase GlfT2 (GalTr 2) (EC 2.4.1.288) (Arabinogalactan galactosyltransferase 2) (Galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase) (Polymerizing galactofuranosyltransferase GlfT2)
Family: Glycosyltransferase 2 family
Protein Sequence: MSELAASLLSRVILPRPGEPLDVRKLYLEESTTNARRAHAPTRTSLQIGAESEVSFATYFNAFPASYWRRWTTCKSVVLRVQVTGAGRVDVYRTKATGARIFVEGHDFTGTEDQPAAVETEVVLQPFEDGGWVWFDITTDTAVTLHSGGWYATSPAPGTANIAVGIPTFNRPADCVNALRELTADPLVDQVIGAVIVPDQGERKVRDHPDFPAAAARLGSRLSIHDQPNLGGSGGYSRVMYEALKNTDCQQILFMDDDIRLEPDSILRVLAMHRFAKAPMLVGGQMLNLQEPSHLHIMGEVVDRSIFMWTAAPHAEYDHDFAEYPLNDNNSRSKLLHRRIDVDYNGWWTCMIPRQVAEELGQPLPLFIKWDDADYGLRAAEHGYPTVTLPGAAIWHMAWSDKDDAIDWQAYFHLRNRLVVAAMHWDGPKAQVIGLVRSHLKATLKHLACLEYSTVAIQNKAIDDFLAGPEHIFSILESALPQVHRIRKSYPDAVVLPAASELPPPLHKNKAMKPPVNPLVIGYRLARGIMHNLTAANPQHHRRPEFNVPTQDARWFLLCTVDGATVTTADGCGVVYRQRDRAKMFALLWQSLRRQRQLLKRFEEMRRIYRDALPTLSSKQKWETALLPAANQEPEHG
Mass: 71,507 Da
Length: 637 Aa

Rv3808c doesn't seem to be a targeted by any drug.

Arabinogalactan biosynthesis - Mycobacterium mtu00572
mtu00572
Metabolism; Glycan biosynthesis and metabolism

Tetrameric structure of the GlfT2 galactofuranosyltransferase reveals a scaffold for the assembly of mycobacterial Arabinogalactan.: J Biol Chem. 2012 Aug 10;287(33):28132-43. doi: 10.1074/jbc.M112.347484. Epub 2012 Jun 15.
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
A tethering mechanism for length control in a processive carbohydrate polymerization.: Proc Natl Acad Sci U S A. 2009 Jul 21;106(29):11851-6. doi: 10.1073/pnas.0901407106. Epub 2009 Jul 1.
Galactosyl transferases in mycobacterial cell wall synthesis.: J Bacteriol. 2008 Feb;190(3):1141-5. doi: 10.1128/JB.01326-07. Epub 2007 Nov 30.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.
Biosynthesis of the galactan component of the mycobacterial cell wall.: J Biol Chem. 2000 Oct 27;275(43):33890-7. doi: 10.1074/jbc.M006875200.
Galactan biosynthesis in Mycobacterium tuberculosis. Identification of a bifunctional UDP-galactofuranosyltransferase.: J Biol Chem. 2001 Jul 13;276(28):26430-40. doi: 10.1074/jbc.M102022200. Epub 2001 Apr 13.
Expression, purification, and characterization of a galactofuranosyltransferase involved in Mycobacterium tuberculosis arabinogalactan biosynthesis.: J Am Chem Soc. 2006 May 24;128(20):6721-9. doi: 10.1021/ja058254d.
targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis.: BMC Syst Biol. 2008 Dec 19;2:109. doi: 10.1186/1752-0509-2-109.
Development of a coupled spectrophotometric assay for GlfT2, a bifunctional mycobacterial galactofuranosyltransferase.: Carbohydr Res. 2008 Aug 11;343(12):2130-9. doi: 10.1016/j.carres.2008.03.023. Epub 2008 Mar 24.