Gene - combatTB X

rraA

Type: protein_coding
Name: rraA
Locus Name: Rv3853
Product: Regulator of RNase E activity a RraA
Functional Category: None
Location: 4325495..4325968 (+ strand)
Gene Length: 473 bp
Nucleotides: TGGCCATCTCATTTCGCCCAACCGCTGACCTCGTCGACGACATCGGGCCCGACGTGCGCAGCTGTGACCTACAGTTCCGCCAATTCGGCGGCCGATCGCAGTTCGCCGGACCGATCAGCACCGTGCGGTGTTTTCAGGACAATGCGTTGCTGAAGTCGGTGCTCTCGCAGCCAAGTGCGGGCGGTGTGCTGGTCATCGACGGCGCCGGGTCCCTGCACACCGCGTTGGTCGGTGATGTCATCGCCGAGTTGGCCCGCTCTACCGGCTGGACCGGGTTGATCGTCCACGGCGCGGTGCGAGATGCCGCCGCGCTGCGCGGCATCGACATCGGCATCAAAGCGCTGGGCACCAATCCCCGCAAGAGCACCAAGACCGGTGCCGGAGAACGCGACGTTGAAATCACGCTGGGCGGGGTGACATTCGTTCCGGGCGATATCGCCTACAGCGACGACGACGGCATCATCGTCGTCTGA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2-oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions (By similarity). {ECO:0000250}.

Family

Class II aldolase/RraA-like family

GO

methylation GO:0032259 biological_process
metal ion binding GO:0046872 molecular_function
ribonuclease inhibitor activity GO:0008428 molecular_function
4-hydroxy-4-methyl-2-oxoglutarate aldolase activity GO:0047443 molecular_function
regulation of RNA metabolic process GO:0051252 biological_process

InterPro

UniProt: P9WGY3
GenBank: Rv3853
EnsemblBacteria: Rv3853
Mycobrowser: Rv3853

Summary

Name: Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase (HMG aldolase) (EC 4.1.3.17) (Oxaloacetate decarboxylase) (OAA decarboxylase) (EC 4.1.1.112) (Regulator of ribonuclease activity homolog) (RraA-like protein)
Family: Class II aldolase/RraA-like family
Protein Sequence: MAISFRPTADLVDDIGPDVRSCDLQFRQFGGRSQFAGPISTVRCFQDNALLKSVLSQPSAGGVLVIDGAGSLHTALVGDVIAELARSTGWTGLIVHGAVRDAAALRGIDIGIKALGTNPRKSTKTGAGERDVEITLGGVTFVPGDIAYSDDDGIIVV
Mass: 16,235 Da
Length: 157 Aa

Rv3853 doesn't seem to be a targeted by any drug.

Rv3853 doesn't seem to be involved in any pathway.

Crystal structure of a putative methyltransferase from Mycobacterium tuberculosis: misannotation of a genome clarified by protein structural analysis.: J Bacteriol. 2003 Jul;185(14):4057-65. doi: 10.1128/jb.185.14.4057-4065.2003.
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis.: BMC Syst Biol. 2008 Dec 19;2:109. doi: 10.1186/1752-0509-2-109.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.