Gene - combatTB X

PPE68

Type: protein_coding
Name: PPE68
Locus Name: Rv3873
Product: PPE family protein PPE68
Functional Category: None
Location: 4351075..4352181 (+ strand)
Gene Length: 1106 bp
Nucleotides: TGCTGTGGCACGCAATGCCACCGGAGCTAAATACCGCACGGCTGATGGCCGGCGCGGGTCCGGCTCCAATGCTTGCGGCGGCCGCGGGATGGCAGACGCTTTCGGCGGCTCTGGACGCTCAGGCCGTCGAGTTGACCGCGCGCCTGAACTCTCTGGGAGAAGCCTGGACTGGAGGTGGCAGCGACAAGGCGCTTGCGGCTGCAACGCCGATGGTGGTCTGGCTACAAACCGCGTCAACACAGGCCAAGACCCGTGCGATGCAGGCGACGGCGCAAGCCGCGGCATACACCCAGGCCATGGCCACGACGCCGTCGCTGCCGGAGATCGCCGCCAACCACATCACCCAGGCCGTCCTTACGGCCACCAACTTCTTCGGTATCAACACGATCCCGATCGCGTTGACCGAGATGGATTATTTCATCCGTATGTGGAACCAGGCAGCCCTGGCAATGGAGGTCTACCAGGCCGAGACCGCGGTTAACACGCTTTTCGAGAAGCTCGAGCCGATGGCGTCGATCCTTGATCCCGGCGCGAGCCAGAGCACGACGAACCCGATCTTCGGAATGCCCTCCCCTGGCAGCTCAACACCGGTTGGCCAGTTGCCGCCGGCGGCTACCCAGACCCTCGGCCAACTGGGTGAGATGAGCGGCCCGATGCAGCAGCTGACCCAGCCGCTGCAGCAGGTGACGTCGTTGTTCAGCCAGGTGGGCGGCACCGGCGGCGGCAACCCAGCCGACGAGGAAGCCGCGCAGATGGGCCTGCTCGGCACCAGTCCGCTGTCGAACCATCCGCTGGCTGGTGGATCAGGCCCCAGCGCGGGCGCGGGCCTGCTGCGCGCGGAGTCGCTACCTGGCGCAGGTGGGTCGTTGACCCGCACGCCGCTGATGTCTCAGCTGATCGAAAAGCCGGTTGCCCCCTCGGTGATGCCGGCGGCTGCTGCCGGATCGTCGGCGACGGGTGGCGCCGCTCCGGTGGGTGCGGGAGCGATGGGCCAGGGTGCGCAATCCGGCGGCTCCACCAGGCCGGGTCTGGTCGCGCCGGCACCGCTCGCGCAGGAGCGTGAAGAAGACGACGAGGACGACTGGGACGAAGAGGACGACTGGTGA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Plays a major role in RD1-associated pathogenesis, and may contribute to the establishment and maintenance of M.tuberculosis infection. Together with PE35, stimulates the secretion of IL-10 and MCP-1 from human macrophages, via the interaction with human Toll-like receptor 2 (TLR2) (PubMed:24467650). Stimulates high levels of gamma interferon secretion in peripheral blood mononuclear cells isolated from tuberculosis patients, as well as from healthy tuberculin purified protein derivative-positive donors. Does not interfere with the secretion and immunogenicity of EsxA (ESAT-6) and EsxB (CFP-10) (PubMed:14573626, PubMed:15039340). {ECO:0000269|PubMed:14573626, ECO:0000269|PubMed:15039340, ECO:0000269|PubMed:24467650}.

Family

Mycobacterial PPE family

GO

plasma membrane GO:0005886 cellular_component
extracellular region GO:0005576 cellular_component
cell wall GO:0005618 cellular_component
pathogenesis GO:0009405 biological_process
cell surface GO:0009986 cellular_component

InterPro

UniProt: P9WHW9
GenBank: Rv3873
EnsemblBacteria: Rv3873
Mycobrowser: Rv3873

Summary

Name: PPE family immunomodulator PPE68
Family: Mycobacterial PPE family
Protein Sequence: MLWHAMPPELNTARLMAGAGPAPMLAAAAGWQTLSAALDAQAVELTARLNSLGEAWTGGGSDKALAAATPMVVWLQTASTQAKTRAMQATAQAAAYTQAMATTPSLPEIAANHITQAVLTATNFFGINTIPIALTEMDYFIRMWNQAALAMEVYQAETAVNTLFEKLEPMASILDPGASQSTTNPIFGMPSPGSSTPVGQLPPAATQTLGQLGEMSGPMQQLTQPLQQVTSLFSQVGGTGGGNPADEEAAQMGLLGTSPLSNHPLAGGSGPSAGAGLLRAESLPGAGGSLTRTPLMSQLIEKPVAPSVMPAAAAGSSATGGAAPVGAGAMGQGAQSGGSTRPGLVAPAPLAQEREEDDEDDWDEEDDW
Mass: 37,330 Da
Length: 368 Aa

Rv3873 doesn't seem to be a targeted by any drug.

Rv3873 doesn't seem to be involved in any pathway.

Characterization of a cross-reactive, immunodominant and HLA-promiscuous epitope of Mycobacterium tuberculosis-specific major antigenic protein PPE68.: PLoS One. 2014 Aug 19;9(8):e103679. doi: 10.1371/journal.pone.0103679. eCollection 2014.
An immunomodulatory role for the Mycobacterium tuberculosis region of difference 1 locus proteins PE35 (Rv3872) and PPE68 (Rv3873).: FEBS J. 2014 Mar;281(6):1556-70. doi: 10.1111/febs.12723. Epub 2014 Feb 12.
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.
PPE protein (Rv3873) from DNA segment RD1 of Mycobacterium tuberculosis: strong recognition of both specific T-cell epitopes and epitopes conserved within the PPE family.: Infect Immun. 2003 Nov;71(11):6116-23. doi: 10.1128/iai.71.11.6116-6123.2003.
Cell envelope protein PPE68 contributes to Mycobacterium tuberculosis RD1 immunogenicity independently of a 10-kilodalton culture filtrate protein and ESAT-6.: Infect Immun. 2004 Apr;72(4):2170-6. doi: 10.1128/iai.72.4.2170-2176.2004.
Dissection of ESAT-6 system 1 of Mycobacterium tuberculosis and impact on immunogenicity and virulence.: Infect Immun. 2006 Jan;74(1):88-98. doi: 10.1128/IAI.74.1.88-98.2006.
A protein linkage map of the ESAT-6 secretion system 1 (ESX-1) of Mycobacterium tuberculosis.: Microbiol Res. 2009;164(3):253-9. doi: 10.1016/j.micres.2006.11.016. Epub 2007 Apr 12.