Gene - combatTB X

espI

Type: protein_coding
Name: espI
Locus Name: Rv3876
Product: ESX-1 secretion-associated protein EspI. Conserved proline and alanine rich protein.
Functional Category: None
Location: 4353010..4355010 (+ strand)
Gene Length: 2000 bp
Nucleotides: TGGCGGCCGACTACGACAAGCTCTTCCGGCCGCACGAAGGTATGGAAGCTCCGGACGATATGGCAGCGCAGCCGTTCTTCGACCCCAGTGCTTCGTTTCCGCCGGCGCCCGCATCGGCAAACCTACCGAAGCCCAACGGCCAGACTCCGCCCCCGACGTCCGACGACCTGTCGGAGCGGTTCGTGTCGGCCCCGCCGCCGCCACCCCCACCCCCACCTCCGCCTCCGCCAACTCCGATGCCGATCGCCGCAGGAGAGCCGCCCTCGCCGGAACCGGCCGCATCTAAACCACCCACACCCCCCATGCCCATCGCCGGACCCGAACCGGCCCCACCCAAACCACCCACACCCCCCATGCCCATCGCCGGACCCGAACCGGCCCCACCCAAACCACCCACACCTCCGATGCCCATCGCCGGACCTGCACCCACCCCAACCGAATCCCAGTTGGCGCCCCCCAGACCACCGACACCACAAACGCCAACCGGAGCGCCGCAGCAACCGGAATCACCGGCGCCCCACGTACCCTCGCACGGGCCACATCAACCCCGGCGCACCGCACCAGCACCGCCCTGGGCAAAGATGCCAATCGGCGAACCCCCGCCCGCTCCGTCCAGACCGTCTGCGTCCCCGGCCGAACCACCGACCCGGCCTGCCCCCCAACACTCCCGACGTGCGCGCCGGGGTCACCGCTATCGCACAGACACCGAACGAAACGTCGGGAAGGTAGCAACTGGTCCATCCATCCAGGCGCGGCTGCGGGCAGAGGAAGCATCCGGCGCGCAGCTCGCCCCCGGAACGGAGCCCTCGCCAGCGCCGTTGGGCCAACCGAGATCGTATCTGGCTCCGCCCACCCGCCCCGCGCCGACAGAACCTCCCCCCAGCCCCTCGCCGCAGCGCAACTCCGGTCGGCGTGCCGAGCGACGCGTCCACCCCGATTTAGCCGCCCAACATGCCGCGGCGCAACCTGATTCAATTACGGCCGCAACCACTGGCGGTCGTCGCCGCAAGCGTGCAGCGCCGGATCTCGACGCGACACAGAAATCCTTAAGGCCGGCGGCCAAGGGGCCGAAGGTGAAGAAGGTGAAGCCCCAGAAACCGAAGGCCACGAAGCCGCCCAAAGTGGTGTCGCAGCGCGGCTGGCGACATTGGGTGCATGCGTTGACGCGAATCAACCTGGGCCTGTCACCCGACGAGAAGTACGAGCTGGACCTGCACGCTCGAGTCCGCCGCAATCCCCGCGGGTCGTATCAGATCGCCGTCGTCGGTCTCAAAGGTGGGGCTGGCAAAACCACGCTGACAGCAGCGTTGGGGTCGACGTTGGCTCAGGTGCGGGCCGACCGGATCCTGGCTCTAGACGCGGATCCAGGCGCCGGAAACCTCGCCGATCGGGTAGGGCGACAATCGGGCGCGACCATCGCTGATGTGCTTGCAGAAAAAGAGCTGTCGCACTACAACGACATCCGCGCACACACTAGCGTCAATGCGGTCAATCTGGAAGTGCTGCCGGCACCGGAATACAGCTCGGCGCAGCGCGCGCTCAGCGACGCCGACTGGCATTTCATCGCCGATCCTGCGTCGAGGTTTTACAACCTCGTCTTGGCTGATTGTGGGGCCGGCTTCTTCGACCCGCTGACCCGCGGCGTGCTGTCCACGGTGTCCGGTGTCGTGGTCGTGGCAAGTGTCTCAATCGACGGCGCACAACAGGCGTCGGTCGCGTTGGACTGGTTGCGCAACAACGGTTACCAAGATTTGGCGAGCCGCGCATGCGTGGTCATCAATCACATCATGCCGGGAGAACCCAATGTCGCAGTTAAAGACCTGGTGCGGCATTTCGAACAGCAAGTTCAACCCGGCCGGGTCGTGGTCATGCCGTGGGACAGGCACATTGCGGCCGGAACCGAGATTTCACTCGACTTGCTCGACCCTATCTACAAGCGCAAGGTCCTCGAATTGGCCGCAGCGCTATCCGACGATTTCGAGAGGGCTGGACGTCGTTGA
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Required to repress ESX-1-mediated secretion under low ATP conditions. This function requires the ATP-binding motif. {ECO:0000269|PubMed:25039394, ECO:0000305|PubMed:14557547}.

Family

Unknown

GO

protein secretion by the type VII secretion system GO:0044315 biological_process
pathogenesis GO:0009405 biological_process
ATP binding GO:0005524 molecular_function
plasma membrane GO:0005886 cellular_component

InterPro

UniProt: P9WJC5
GenBank: Rv3876
EnsemblBacteria: Rv3876
Mycobrowser: Rv3876

Summary

Name: ESX-1 secretion-associated protein EspI
Family: Unknown
Protein Sequence: MAADYDKLFRPHEGMEAPDDMAAQPFFDPSASFPPAPASANLPKPNGQTPPPTSDDLSERFVSAPPPPPPPPPPPPPTPMPIAAGEPPSPEPAASKPPTPPMPIAGPEPAPPKPPTPPMPIAGPEPAPPKPPTPPMPIAGPAPTPTESQLAPPRPPTPQTPTGAPQQPESPAPHVPSHGPHQPRRTAPAPPWAKMPIGEPPPAPSRPSASPAEPPTRPAPQHSRRARRGHRYRTDTERNVGKVATGPSIQARLRAEEASGAQLAPGTEPSPAPLGQPRSYLAPPTRPAPTEPPPSPSPQRNSGRRAERRVHPDLAAQHAAAQPDSITAATTGGRRRKRAAPDLDATQKSLRPAAKGPKVKKVKPQKPKATKPPKVVSQRGWRHWVHALTRINLGLSPDEKYELDLHARVRRNPRGSYQIAVVGLKGGAGKTTLTAALGSTLAQVRADRILALDADPGAGNLADRVGRQSGATIADVLAEKELSHYNDIRAHTSVNAVNLEVLPAPEYSSAQRALSDADWHFIADPASRFYNLVLADCGAGFFDPLTRGVLSTVSGVVVVASVSIDGAQQASVALDWLRNNGYQDLASRACVVINHIMPGEPNVAVKDLVRHFEQQVQPGRVVVMPWDRHIAAGTEISLDLLDPIYKRKVLELAAALSDDFERAGRR
Mass: 70,645 Da
Length: 666 Aa

Rv3876 doesn't seem to be a targeted by any drug.

Rv3876 doesn't seem to be involved in any pathway.

Recombinant preparation and functional studies of EspI ATP binding domain from Mycobacterium tuberculosis.: Protein Expr Purif. 2016 Jul;123:51-9. doi: 10.1016/j.pep.2016.03.009. Epub 2016 Mar 25.
EspI regulates the ESX-1 secretion system in response to ATP levels in Mycobacterium tuberculosis.: Mol Microbiol. 2014 Sep;93(5):1057-1065. doi: 10.1111/mmi.12718. Epub 2014 Jul 31.
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Conservation of structure and protein-protein interactions mediated by the secreted mycobacterial proteins EsxA, EsxB, and EspA.: J Bacteriol. 2010 Jan;192(1):326-35. doi: 10.1128/JB.01032-09.
Systematic genetic nomenclature for type VII secretion systems.: PLoS Pathog. 2009 Oct;5(10):e1000507. doi: 10.1371/journal.ppat.1000507. Epub 2009 Oct 30.
Dissection of ESAT-6 system 1 of Mycobacterium tuberculosis and impact on immunogenicity and virulence.: Infect Immun. 2006 Jan;74(1):88-98. doi: 10.1128/IAI.74.1.88-98.2006.
Individual RD1-region genes are required for export of ESAT-6/CFP-10 and for virulence of Mycobacterium tuberculosis.: Mol Microbiol. 2004 Jan;51(2):359-70. doi: 10.1046/j.1365-2958.2003.03844.x.
The primary mechanism of attenuation of bacillus Calmette-Guerin is a loss of secreted lytic function required for invasion of lung interstitial tissue.: Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):12420-5. doi: 10.1073/pnas.1635213100. Epub 2003 Oct 13.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.