Gene - combatTB X

espB

Type: protein_coding
Name: espB
Locus Name: Rv3881c
Product: Secreted ESX-1 substrate protein B, EspB. Conserved alanine and glycine rich protein
Functional Category: None
Location: 4360543..4361925 (- strand)
Gene Length: 1382 bp
Nucleotides: ATGACGCAGTCGCAGACCGTGACGGTGGATCAGCAAGAGATTTTGAACAGGGCCAACGAGGTGGAGGCCCCGATGGCGGACCCACCGACTGATGTCCCCATCACACCGTGCGAACTCACGGCGGCTAAAAACGCCGCCCAACAGCTGGTATTGTCCGCCGACAACATGCGGGAATACCTGGCGGCCGGTGCCAAAGAGCGGCAGCGTCTGGCGACCTCGCTGCGCAACGCGGCCAAGGCGTATGGCGAGGTTGATGAGGAGGCTGCGACCGCGCTGGACAACGACGGCGAAGGAACTGTGCAGGCAGAATCGGCCGGGGCCGTCGGAGGGGACAGTTCGGCCGAACTAACCGATACGCCGAGGGTGGCCACGGCCGGTGAACCCAACTTCATGGATCTCAAAGAAGCGGCAAGGAAGCTCGAAACGGGCGACCAAGGCGCATCGCTCGCGCACTTTGCGGATGGGTGGAACACTTTCAACCTGACGCTGCAAGGCGACGTCAAGCGGTTCCGGGGGTTTGACAACTGGGAAGGCGATGCGGCTACCGCTTGCGAGGCTTCGCTCGATCAACAACGGCAATGGATACTCCACATGGCCAAATTGAGCGCTGCGATGGCCAAGCAGGCTCAATATGTCGCGCAGCTGCACGTGTGGGCTAGGCGGGAACATCCGACTTATGAAGACATAGTCGGGCTCGAACGGCTTTACGCGGAAAACCCTTCGGCCCGCGACCAAATTCTCCCGGTGTACGCGGAGTATCAGCAGAGGTCGGAGAAGGTGCTGACCGAATACAACAACAAGGCAGCCCTGGAACCGGTAAACCCGCCGAAGCCTCCCCCCGCCATCAAGATCGACCCGCCCCCGCCTCCGCAAGAGCAGGGATTGATCCCTGGCTTCCTGATGCCGCCGTCTGACGGCTCCGGTGTGACTCCCGGTACCGGGATGCCAGCCGCACCGATGGTTCCGCCTACCGGATCGCCGGGTGGTGGCCTCCCGGCTGACACGGCGGCGCAGCTGACGTCGGCTGGGCGGGAAGCCGCAGCGCTGTCGGGCGACGTGGCGGTCAAAGCGGCATCGCTCGGTGGCGGTGGAGGCGGCGGGGTGCCGTCGGCGCCGTTGGGATCCGCGATCGGGGGCGCCGAATCGGTGCGGCCCGCTGGCGCTGGTGACATTGCCGGCTTAGGCCAGGGAAGGGCCGGCGGCGGCGCCGCGCTGGGCGGCGGTGGCATGGGAATGCCGATGGGTGCCGCGCATCAGGGACAAGGGGGCGCCAAGTCCAAGGGTTCTCAGCAGGAAGACGAGGCGCTCTACACCGAGGATCGGGCATGGACCGAGGCCGTCATTGGTAACCGTCGGCGCCAGGACAGTAAGGAGTCGAAGTG
Drug Resistance: Check for drug resistance association at TBDREAMDB
Mutations: Check for mutants available at TARGET

Function

Required for host-cell death and may support an EsxA-independent virulence function (PubMed:23869560). Secreted processed form of EspB binds to phosphatidic acid and phosphatidylserine (PubMed:23869560). Inhibits IFN-gamma-induced autophagy in murine macrophages (PubMed:25641595). {ECO:0000269|PubMed:23869560, ECO:0000269|PubMed:25641595}.

Family

EspB family

GO

protein secretion by the type VII secretion system GO:0044315 biological_process
pathogenesis GO:0009405 biological_process
extracellular region GO:0005576 cellular_component
identical protein binding GO:0042802 molecular_function

InterPro

IPR041275

UniProt: P9WJD9
GenBank: Rv3881c
EnsemblBacteria: Rv3881c
Mycobrowser: Rv3881c

Summary

Name: ESX-1 secretion-associated protein EspB (Antigen MTB48)
Family: EspB family
Protein Sequence: MTQSQTVTVDQQEILNRANEVEAPMADPPTDVPITPCELTAAKNAAQQLVLSADNMREYLAAGAKERQRLATSLRNAAKAYGEVDEEAATALDNDGEGTVQAESAGAVGGDSSAELTDTPRVATAGEPNFMDLKEAARKLETGDQGASLAHFADGWNTFNLTLQGDVKRFRGFDNWEGDAATACEASLDQQRQWILHMAKLSAAMAKQAQYVAQLHVWARREHPTYEDIVGLERLYAENPSARDQILPVYAEYQQRSEKVLTEYNNKAALEPVNPPKPPPAIKIDPPPPPQEQGLIPGFLMPPSDGSGVTPGTGMPAAPMVPPTGSPGGGLPADTAAQLTSAGREAAALSGDVAVKAASLGGGGGGGVPSAPLGSAIGGAESVRPAGAGDIAGLGQGRAGGGAALGGGGMGMPMGAAHQGQGGAKSKGSQQEDEALYTEDRAWTEAVIGNRRRQDSKESK
Mass: 47,594 Da
Length: 460 Aa

Rv3881c doesn't seem to be a targeted by any drug.

Rv3881c doesn't seem to be involved in any pathway.

Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism.: Structure. 2015 Mar 3;23(3):571-583. doi: 10.1016/j.str.2015.01.002. Epub 2015 Feb 12.
Structure of EspB, a secreted substrate of the ESX-1 secretion system of Mycobacterium tuberculosis.: J Struct Biol. 2015 Aug;191(2):236-44. doi: 10.1016/j.jsb.2015.06.003. Epub 2015 Jun 4.
Anticytolytic screen identifies inhibitors of mycobacterial virulence protein secretion.: Cell Host Microbe. 2014 Oct 8;16(4):538-48. doi: 10.1016/j.chom.2014.09.008.
Mycobacterium tuberculosis EspB protein suppresses interferon-gamma-induced autophagy in murine macrophages.: J Microbiol Immunol Infect. 2016 Dec;49(6):859-865. doi: 10.1016/j.jmii.2014.11.008. Epub 2014 Nov 21.
Mycobacterium tuberculosis EspB binds phospholipids and mediates EsxA-independent virulence.: Mol Microbiol. 2013 Sep;89(6):1154-66. doi: 10.1111/mmi.12336. Epub 2013 Aug 15.
Understanding specificity of the mycosin proteases in ESX/type VII secretion by structural and functional analysis.: J Struct Biol. 2013 Nov;184(2):115-28. doi: 10.1016/j.jsb.2013.09.022. Epub 2013 Oct 7.
Structure of the mycosin-1 protease from the mycobacterial ESX-1 protein type VII secretion system.: J Biol Chem. 2013 Jun 14;288(24):17782-90. doi: 10.1074/jbc.M113.462036. Epub 2013 Apr 25.
Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.: Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
Mycobacterium tuberculosis MycP1 protease plays a dual role in regulation of ESX-1 secretion and virulence.: Cell Host Microbe. 2010 Mar 18;7(3):210-20. doi: 10.1016/j.chom.2010.02.006.
A mycobacterium ESX-1-secreted virulence factor with unique requirements for export.: PLoS Pathog. 2007 Aug 3;3(8):e105. doi: 10.1371/journal.ppat.0030105.
The regulatory elements of the Mycobacterium tuberculosis gene Rv3881c function efficiently in Escherichia coli.: FEMS Microbiol Lett. 2003 Jan 28;218(2):365-70. doi: 10.1016/S0378-1097(02)01185-0.
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.: Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.
Serological expression cloning and immunological evaluation of MTB48, a novel Mycobacterium tuberculosis antigen.: J Clin Microbiol. 2001 Jul;39(7):2485-93. doi: 10.1128/JCM.39.7.2485-2493.2001.