Rv3903c
- Type
- protein_coding
- Name
- Rv3903c
- Locus Name
-
Rv3903c
- Product
-
Hypothetical alanine and proline rich protein
- Functional Category
-
None
- Location
- 4387892..4390432 (- strand)
- Gene Length
- 2540 bp
- Nucleotides
-
ATGGCGCCGTTGGCGGTCGATCCCGCGGCCCTTGATAGCGCGGGCGGCGCGGTGGTGGCTGCGGGTGCGGGTTTGGGTGCGGTGATCTCGTCGCTGACCGCGGCGCTGGCCGGGTGTGCGGGGATGGCCGGTGATGATCCGGCTGGGGCGGTGTTCGGGCGCTCCTATGACGGTTCGGCGGCCGCGCTGGTGCAGGCGATGTCGGTGGCGCGCAACGGATTGTGCAACCTCGGCGATGGGGTGCGCATGAGCGCGCACAACTACTCGTTGGCCGAGGCGATGTCGGATGTCGCTGGGCGGGCGGCGCCGTTGCCGGCGCCGCCGCCGAGCGGCTGTGTCGGCGTGGGTGCGCCGCCGTCGGCGGTCGGTGGCGGCGGTGGCGCCCCGAAGGGCTGGGGGTGGGTGGCCCCGTATATCGGGATGATCTGGCCGAACGGGGATTCGACAAAGCTACGTGCGGCGGCTGTGGCGTGGCGCAGCGCGGGCACGCAGTTCGCGCTGACTGAGATTCAGTCGACGGCGGGGCCGATGGGCGTTATTCGCGCCCAGCAGCTCCCGGAGGCGGGGCTGATCGAGTCGGCGTTTGCTGACGCGTACGCCAGCACCACCGCTGTCGTGGGCCAATGCCACCAGCTGGCGGCCCAGCTAGACGCCTATGCCGCCCGCATCGACGCGGTGCATGCGGCGGTCCTGGATTTGTTGGCCCGCATCTGCGATCCGCTGACCGGGATCAAAGAGGTGTGGGAGTTTCTGACCGACCAGGACGAAGACGAGATCCAGCGCATCGCCCATGACATCGCGGTGGTGGTCGACCAGTTCAGCGGGGAAGTGGACGCGTTGGCTGCGGAGATCACCGCGGTGGTGTCGCACGCCGAGGCGGTGATCACCGCGATGGCAGACCACGCAGGCAAACAATGGGATCGGTTCTTGCACAGCAACCCGGTGGGTGTGGTCATCGATGGCACCGGGCAGCAGCTCAAAGGCTTCGGCGAGGAGGCCTTCGGGATGGCCAAGGACTCCTGGGACCTAGGGCCACTGCGCGCCTCGATAGACCCGTTCGGGTGGTATCGCTCCTGGGAGGAGATGCTGACTGGGATGGCGCCGCTGGCGGGCCTGGGCGGCGAGAACGCTCCCGGCGTTGTGGAGTCGTGGAAGCAGTTCGGCAAAAGCCTCATCCATTGGGATGAGTGGACGACCAACCCTAATGAGGCGCTAGGCAAGACCGTATTCGACGCCGCGACGCTAGCTTTGCCGGGCGGGCCGCTGTCGAAACTTGGCAGCAAGGGCCGCGACATTCTCGCGGGCGTGCGAGGCCTCAAGGAGCGGCTTGAGCCGACGACACCGCACCTTGAGCCCCCAGCAACGCCGCCGCGGCCAGGACCGCAACCACCACGGATCGAACCACCAGAATCGGGCCACCCGGCACCCGCGCCCGCGGCGAAACCGGCGCCCGTGCCCGCCAACGGTCCACTGCCGCACAGCCCCACCGAATCCAAACCGCCGCCCGTCGACAGACCGGCTGAACCGGTGGCGCCGTCGTCGGCGTCGGCAGGCCAGCCCCGGGTATCCGCAGCCACCACGCCCGGCACACATGTGCCGCATGGCCTGCCGCAACCGGGTGAACATGTCCCGGCGCAAGCACCACCGGCGACGACGTTGCTTGGCGGACCTCCTGTCGAGTCAGCGCCCGCTACCGCGCACCAACCCCAGTGGGCGACCACACCAGCAGCACCCGCGGCGGCGCCGCATTCCACGCCCGGAGGCGTTCACTCAACCGAATCGGGGCCTCACGGCCGATCACTGAGCGCACACGGATCCGAGCCGACCCACGACGGTGCGTCTCACGGGTCAGGCCACGGTAGCGGTAGCGAGCCACCTGGGCTGCATGCGCCGCATCGCGAGCAGCAGCTTGCAATGCATTCGAACGAACCAGCTGGAGAGGGTTGGCATCGGTTATCCGACGAAGCGGTTGACCCGCAGTATGGCGAGCCATTATCGCGCCACTGGGACTTTACAGACAATCCGGCCGATCGCAGTCGGATAAACCCGGTTGTGGCCCAGCTCATGGAGGACCCCAACGCCCCATTCGGCCGCGATCCTCAGGGACAGCCCTATACCCAAGAACGGTATCAAGAGCGATTTAATAGTGTAGGCCCATGGGGCCAGCAGTACTCTAATTTTCCGCCTAACAATGGTGCGGTTCCAGGGACAAGGATCGCCTACACTAATCTCGAAAAATTTCTTAGTGACTACGGCCCCCAGCTAGATCGTATAGGCGGCGATCAGGGCAAGTACCTGGCGATCATGGAACATGGGCGCCCGGCATCATGGGAACAACGTGCCCTGCACGTGACGTCGTTACGCGACCCCTACCACGCGTATACCATTGATTGGTTGCCTGAGGGATGGTTCATCGAAGTATCTGAGGTCGCGCCGGGGTGCGGCCAGCCGGGCGGATCCATCCAAGTGCGGATATTCGATCATCAGAACGAGATGCGCAAAGTGGAAGAGTTAATAAGGCGCGGGGTGTTGCGACAGTG
- Drug Resistance
- Mutations
- Function
- Has a dual function in uptake of nutrients and induction of host cell death. The N-terminal domain (NTD) forms an outer membrane channel and is used for uptake of nutrients across the outer membrane. The secreted C-terminal toxic domain (TNT) acts as a glycohydrolase, which hydrolyzes the essential cellular coenzyme NAD(+) in the cytosol of infected macrophages, leading to necrotic host cell death. Both functions are required for survival, replication and cytotoxicity of M.tuberculosis in macrophages. {ECO:0000269|PubMed:24753609, ECO:0000269|PubMed:26237511}.
- Family
-
Unknown
- GO
-
- pore complex GO:0046930 cellular_component
- cell surface GO:0009986 cellular_component
- ion transport GO:0006811 biological_process
- toxin activity GO:0090729 molecular_function
- porin activity GO:0015288 molecular_function
- extracellular region GO:0005576 cellular_component
- cell wall GO:0005618 cellular_component
- cell outer membrane GO:0009279 cellular_component
- NAD glycohydrolase activity GO:0061810 molecular_function
- host cell cytosol GO:0044164 cellular_component
- InterPro
4QLP
- Name
- Outer membrane channel protein CpnT (Channel protein with necrosis-inducing toxin) [Cleaved into: N-terminal channel domain; Tuberculosis necrotizing toxin (TNT) (NAD(+) glycohydrolase) (EC 3.2.2.5)]
- Family
- Unknown
- Protein Sequence
-
MAPLAVDPAALDSAGGAVVAAGAGLGAVISSLTAALAGCAGMAGDDPAGAVFGRSYDGSAAALVQAMSVARNGLCNLGDGVRMSAHNYSLAEAMSDVAGRAAPLPAPPPSGCVGVGAPPSAVGGGGGAPKGWGWVAPYIGMIWPNGDSTKLRAAAVAWRSAGTQFALTEIQSTAGPMGVIRAQQLPEAGLIESAFADAYASTTAVVGQCHQLAAQLDAYAARIDAVHAAVLDLLARICDPLTGIKEVWEFLTDQDEDEIQRIAHDIAVVVDQFSGEVDALAAEITAVVSHAEAVITAMADHAGKQWDRFLHSNPVGVVIDGTGQQLKGFGEEAFGMAKDSWDLGPLRASIDPFGWYRSWEEMLTGMAPLAGLGGENAPGVVESWKQFGKSLIHWDEWTTNPNEALGKTVFDAATLALPGGPLSKLGSKGRDILAGVRGLKERLEPTTPHLEPPATPPRPGPQPPRIEPPESGHPAPAPAAKPAPVPANGPLPHSPTESKPPPVDRPAEPVAPSSASAGQPRVSAATTPGTHVPHGLPQPGEHVPAQAPPATTLLGGPPVESAPATAHQPQWATTPAAPAAAPHSTPGGVHSTESGPHGRSLSAHGSEPTHDGASHGSGHGSGSEPPGLHAPHREQQLAMHSNEPAGEGWHRLSDEAVDPQYGEPLSRHWDFTDNPADRSRINPVVAQLMEDPNAPFGRDPQGQPYTQERYQERFNSVGPWGQQYSNFPPNNGAVPGTRIAYTNLEKFLSDYGPQLDRIGGDQGKYLAIMEHGRPASWEQRALHVTSLRDPYHAYTIDWLPEGWFIEVSEVAPGCGQPGGSIQVRIFDHQNEMRKVEELIRRGVLRQ
- Mass
- 88,335 Da
- Length
- 846 Aa
Summary
Rv3903c doesn't seem to be a targeted by any drug.
Rv3903c doesn't seem to be involved in any pathway.
- The tuberculosis necrotizing toxin kills macrophages by hydrolyzing NAD.
- Nat Struct Mol Biol. 2015 Sep;22(9):672-8. doi: 10.1038/nsmb.3064. Epub 2015 Aug 3.
- The Mycobacterium tuberculosis outer membrane channel protein CpnT confers susceptibility to toxic molecules.
- Antimicrob Agents Chemother. 2015 Apr;59(4):2328-36. doi: 10.1128/AAC.04222-14. Epub 2015 Feb 2.
- An outer membrane channel protein of Mycobacterium tuberculosis with exotoxin activity.
- Proc Natl Acad Sci U S A. 2014 May 6;111(18):6750-5. doi: 10.1073/pnas.1400136111. Epub 2014 Apr 21.
- Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry.
- Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3.
- Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.
- Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.