Uncharacterized protein Rv0498

Protein

Unknown.

P9WKU1

Uncharacterized protein Rv0502

Protein

Unknown.

P9WKT1

Potassium-transporting ATPase KdpC subunit (ATP phosphohydrolase [potassium-transporting] C chain) (Potassium-binding and translocating subunit C) (Potassium-translocating ATPase C chain)

Protein

Part of the high-affinity ATP-driven potassium transport (or Kdp) system, which catalyzes the hydrolysis of ATP coupled with the electrogenic transport of potassium into the cytoplasm. This subunit acts as a catalytic chaperone that increases the ATP-binding affinity of the ATP-hydrolyzing subunit KdpB by the formation of a transient KdpB/KdpC/ATP ternary complex. {ECO:0000255|HAMAP-Rule:MF_00276}..

P9WKF1

4-hydroxy-3-methylbut-2-enyl diphosphate reductase 2 (HDR 2) (HMBPP reductase 2) (EC 1.17.7.4)

Protein

Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) (PubMed:23091471). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis. Has a higher activity compared with LytB2 (PubMed:23091471). Is essential for M.tuberculosis growth in vitro (PubMed:26309039). {ECO:0000255|HAMAP-Rule:MF_00191, ECO:0000269|PubMed:23091471, ECO:0000269|PubMed:26309039}..

P9WKG1

Uncharacterized protein Rv3466

Protein

Unknown.

P9WKY1

Uncharacterized protein Rv3630

Protein

Unknown.

P9WKX9

Bacterial proteasome activator

Protein

Interacts with the core proteasome alpha-subunit (PrcA) through its C-terminal hydrophobic-tyrosine-X motif (HbYX motif). Interaction of Bpa with the proteasome stimulates proteosomal peptidase and casein degradation activity, which suggests Bpa could play a role in the removal of non-native or damaged proteins by influencing the conformation of the proteasome complex upon interaction. Can inhibit degradation of Pup-tagged substrates in vitro by competing with Mpa for association with the proteasome. {ECO:0000269|PubMed:25469515}..

P9WKX3

Methionine aminopeptidase 1 (MAP 1) (MetAP 1) (EC 3.4.11.18) (Peptidase M)

Protein

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed. {ECO:0000255|HAMAP-Rule:MF_01974, ECO:0000269|PubMed:19688379}..

P9WK21

Uncharacterized protein Rv0940c

Protein

Unknown.

P9WKP1

Uncharacterized protein Rv0959

Protein

Unknown.

P9WKN1

Lipoprotein LprI (Glycolipoprotein LprI) (Lysozyme inhibitor LprI)

Protein

Strongly binds and inhibits lysozyme, may help bacteria survive in lysozyme-producing host cells. When overexpressed in M.tuberculosis or M.smegmatis increases resistance to hen egg white lysozyme. M.smegmatis overexpressing LprI survive better during intracellular infection of peritoneal and monocyte-derived macrophages, both of which produce lysozyme during infection; M.smegmatis does not encode this protein. Somewhat better survival is seen in human cell lines when M.smegmatis cells express both proteins from this operon, i.e. GlbN (HbN) and LprI. {ECO:0000269|PubMed:26589796}..

P9WK41

Putative diacyglycerol O-acyltransferase Rv1425 (EC 2.3.1.20) (Putative triacylglycerol synthase Rv1425)

Protein

Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis (By similarity). {ECO:0000250}.; FUNCTION: Upon expression in E.coli functions very weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has no wax synthase activity..

P9WKC1

Putative lipoprotein LppA

Protein

Unknown.

P9WK81

Translation initiation factor IF-2

Protein

One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex (By similarity). {ECO:0000250}..

P9WKK1

Lipoprotein LpqH (19 kDa lipoprotein antigen) (Putative transporter LpqH) (p19)

Protein

Based on its structure might be involved in ligand transport (Ref.25) (By similarity). {ECO:0000250|UniProtKB:P65307, ECO:0000305|Ref.25}.; FUNCTION: A host TLR2 agonist (PubMed:10426995, PubMed:11441098, PubMed:12874328). Plays a complicated role in bacterial interactions with the host immune system; some effects favor the host (induces interleukin 1-beta and IL-12 p40 (IL12B), both increase the host's immune response) while others favor the bacteria (increases growth in monocyte-derived macrophages and decreases host MHC class II (MHC-II) expression and antigen processing) (PubMed:16177361). Induces host (human and mouse) IL-12 p40 (IL12B, a proinflammatory cytokine) release by monocyte cell lines via TLR2 and CD14 (PubMed:10426995). Induces host (human) monocytes to produce TNF-alpha, IL-6 and IL-12 p40; LpqH is a more potent inducer than PstS1 (PubMed:16622205). Inhibits MHC-II expression and antigen processing in host (mouse) macrophages via TLR2 (independently of TLR4) probably via the lipid modification (PubMed:11441098). Stimulates host (human) dendritic cell maturation to become MHC-II-positive antigen presenting cells via TLR2, which depends on lipidation; nonlipidated protein does not stimulate maturation (PubMed:11160304). Inhibits host (human and mouse) IFN-gamma signaling in macrophages via TLR2; decreases IFN-gamma stimulated MHC-II antigen processing as well as decreasing IFN-gamma-mediated up-regulation of immunoglobulin gamma Fc receptor (FCGR1A), enabling the bacteria to evade the immune system (PubMed:12874328). In resting human CD4+ T-cells lipidated (but probably not nonlipidated protein) is a costimulatory ligand (with anti-CD3 and anti-CD28) for T-cell proliferation and IFN-gamma and IL-2 production (PubMed:21078852). Human CD4+ T-cells probably use TLR1/TLR2 heterodimers to respond to mycobacterial lipoproteins (PubMed:21078852). Acting via TLR2 enhances expression of host peroxisome proliferator-activated receptor gamma (PPARG), a regulator of inflammation and immunoregulation, and increases p38 MAPK phosphorylation, IL-6 and TNF-alpha expression (PubMed:25504154). Native or nonlipidated recombinant protein missing the first 4 residues have been shown to induce apoptosis in the human macrophage cell line THP-1 and human monocyte-derived macrophages in a TLR2, caspase-3 and caspase-8-dependent manner (PubMed:12594264). Protein overexpressed in M.smegmatis (lipidated and probably glycosylated) induces apoptosis in human macrophages via TLR2 in a caspase-3/caspase-8-mediated manner, but also in a caspase-independent manner where mitochondrial apoptosis-inducing factor (AIFM1) translocates to the nucleus (PubMed:23316255). Another study found mature, native (lipidated) protein did not induce apoptosis in THP-1 macrophage cell line (PubMed:12874328). Functions as an adhesin, binds to human and mouse macrophages (PubMed:25359607). {ECO:0000269|PubMed:10426995, ECO:0000269|PubMed:11160304, ECO:0000269|PubMed:11441098, ECO:0000269|PubMed:12594264, ECO:0000269|PubMed:12874328, ECO:0000269|PubMed:16177361, ECO:0000269|PubMed:16622205, ECO:0000269|PubMed:21078852, ECO:0000269|PubMed:23316255, ECO:0000269|PubMed:25359607, ECO:0000269|PubMed:25504154}..

P9WK61

Uncharacterized protein Rv3785

Protein

Unknown.

P9WKX1

Uncharacterized protein Rv0966c

Protein

Unknown.

P9WKM1

Salicylate synthase (Chorismate mutase) (CM) (EC 5.4.99.5) (Isochorismate synthase/isochorismate lyase) (EC 4.2.99.21) (EC 5.4.4.2) (Mycobactin synthase protein)

Protein

Involved in the incorporation of salicylate into the virulence-conferring salicylate-based siderophore mycobactin. Catalyzes the initial conversion of chorismate to yield the intermediate isochorismate (isochorismate synthase activity), and the subsequent elimination of the enolpyruvyl side chain to give salicylate (isochorismate pyruvate-lyase activity). In the absence of magnesium, MbtI displays a chorismate mutase activity and converts chorismate to prephenate. {ECO:0000269|PubMed:16923875, ECO:0000269|PubMed:17240979, ECO:0000269|PubMed:20487026, ECO:0000269|PubMed:20512795, ECO:0000269|PubMed:9831524}..

P9WFX1

Ribosomal RNA small subunit methyltransferase E (EC 2.1.1.193) (16S rRNA m3U1498 methyltransferase)

Protein

Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit (By similarity). {ECO:0000250}..

P9WGX1

Uncharacterized MFS-type transporter Rv2456c

Protein

Unknown.

P9WJX1

Putative diacyglycerol O-acyltransferase Rv3087 (EC 2.3.1.20) (Putative triacylglycerol synthase Rv3087)

Protein

Required for maintaining the appropriate mycolic acid composition and permeability of the envelope on its exposure to acidic pH. Upon expression in E.coli functions weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has no wax synthase activity. {ECO:0000269|PubMed:15262939, ECO:0000269|PubMed:15937179}..

P9WKB1

(2E,6E)-farnesyl diphosphate synthase (E,E-FPP synthase) (FPP synthase) (EC 2.5.1.10)

Protein

Catalyzes the condensation of isopentenyl pyrophosphate (IPP) with geranyl diphosphate (GPP) to yield (2E,6E)-farnesyl diphosphate (E,E-FPP). May be used for squalene and possibly sterol biosynthesis. {ECO:0000269|PubMed:15060088}..

P9WKH1

NADH-quinone oxidoreductase subunit H (EC 7.1.1.-) (NADH dehydrogenase I subunit H) (NDH-1 subunit H)

Protein

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. {ECO:0000255|HAMAP-Rule:MF_01350}..

P9WIX1

Isocitrate dehydrogenase [NADP] (IDH) (EC 1.1.1.42) (IDP) (NADP(+)-specific ICDH) (Oxalosuccinate decarboxylase)

Protein

Unknown.

P9WKL1

Uncharacterized methyltransferase Rv3342 (EC 2.1.1.-)

Protein

Unknown.

P9WK01

Uncharacterized PPE family protein PPE66

Protein

Unknown.

P9WHX1

Transcriptional regulator Rv0485

Protein

Positively regulates the expression of PE13 and PPE18. Can also regulate expression of some other genes. Plays a role in modulation of innate immune responses. {ECO:0000269|PubMed:19651861}..

P9WKV1

Uncharacterized protein Rv0739

Protein

Unknown.

P9WKS1

2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (SEPHCHC synthase) (EC 2.2.1.9) (Menaquinone biosynthesis protein MenD)

Protein

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC). {ECO:0000255|HAMAP-Rule:MF_01659}..

P9WK11

Sphingomyelinase (SMase) (EC 3.1.4.12)

Protein

Catalyzes the cleavage of sphingomyelin, a major lipid in eukaryotic cells, into ceramide and phosphocholine, which are then utilized by M.tuberculosis as carbon, nitrogen and phosphorus sources, respectively. Thus, enables M.tuberculosis to utilize sphingomyelin as a source of several essential nutrients for intracellular growth during infection. Furthermore, lyses erythrocytes and constitutes the main hemolytic factor of M.tuberculosis. {ECO:0000269|PubMed:26036301}..

P9WKQ1

Uncharacterized protein Rv1490

Protein

Unknown.

P9WLX1

Putative lipoprotein LprD

Protein

Unknown.

P9WK51

Lipoyl synthase (EC 2.8.1.8) (Lip-syn) (LS) (Lipoate synthase) (Lipoic acid synthase) (Sulfur insertion protein LipA)

Protein

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives. {ECO:0000255|HAMAP-Rule:MF_00206}..

P9WK91

Signal peptidase I (SPase I) (EC 3.4.21.89) (Leader peptidase I)

Protein

Unknown.

P9WKA1

Alpha-ketoglutarate-dependent sulfate ester dioxygenase (EC 1.14.11.-) (Type II alkyl sulfatase)

Protein

Alpha-ketoglutarate-dependent sulfate ester dioxygenase, which oxidizes medium-chain alkyl-sulfate esters (PubMed:23762287). Shows preference for 2-ethylhexyl sulfate (2-EHS) in vitro, leading to the formation of succinate and 2-ethylhexanal (PubMed:23762287, PubMed:25427196). Has likely a role in sulfate scavenging in vivo (PubMed:23762287). {ECO:0000269|PubMed:23762287, ECO:0000269|PubMed:25427196}.; FUNCTION: Also causes the inactivation of the 2-carboxyquinoxaline Ty38c (an antitubercular compound that inhibits DprE1) via oxidative decarboxylation, using Ty38c instead of alpha-ketoglutarate as a substrate. Is thus responsible for primary resistance of M.tuberculosis to Ty38c in vitro. Overexpression of Rv3406 causes resistance to Ty38c. {ECO:0000269|PubMed:25427196}..

P9WKZ1

Uncharacterized protein Rv3660c

Protein

May play a role in septum formation. {ECO:0000269|PubMed:16735741}..

P9WKX7

Penicillin-binding protein A (PBPA)

Protein

Cell wall formation. Plays an important role in cell division and cell shape maintenance by cross-linking adjacent peptidoglycan chains through transpeptidation..

P9WKD1

Inositol-3-phosphate synthase (IPS) (EC 5.5.1.4) (Myo-inositol 1-phosphate synthase) (MI-1-P synthase) (MIP synthase)

Protein

Catalyzes the conversion of glucose 6-phosphate to 1D-myo-inositol 3-phosphate. {ECO:0000250}..

P9WKI1

Uncharacterized protein Rv0476

Protein

Unknown.

P9WKW1

Putative mycofactocin biosynthesis glycosyltransferase MftF (EC 2.-.-.-)

Protein

May play a role in the maturation of mycofactocin, a conserved polypeptide that might serve as an electron carrier. The genes for mycofactocin and other proteins proposed to function in its maturation are found in a conserved gene cluster..

P9WMX1

Uncharacterized protein Rv0879c

Protein

Unknown.

P9WKR1

Homoserine dehydrogenase (HDH) (EC 1.1.1.3)

Protein

Unknown.

P9WPX1

Putative amino-acid transporter Rv1986

Protein

Unknown.

P9WK31

3'-phosphoadenosine 5'-phosphate phosphatase (PAP phosphatase) (EC 3.1.3.7) (3'(2'),5'-bisphosphate nucleotidase) (3'(2'),5-bisphosphonucleoside 3'(2')-phosphohydrolase) (D-fructose-1,6-bisphosphate 1-phosphohydrolase) (DPNPase) (Fructose-1,6-bisphosphatase) (FBPase) (EC 3.1.3.11) (Inositol-1-monophosphatase) (I-1-Pase) (IMPase) (EC 3.1.3.25) (Inositol-1-phosphatase)

Protein

Phosphatase with a broad specificity. Its primary physiological function is to dephosphorylate 3'-phosphoadenosine 5'-phosphate (PAP) and 3'-phosphoadenosine 5'-phosphosulfate (PAPS). Thus, plays a role in mycobacterial sulfur metabolism, since it can serve as a key regulator of the sulfate assimilation pathway by controlling the pools of PAP and PAPS in the cell. To a lesser extent, is also able to hydrolyze inositol 1-phosphate (I-1-P), fructose 1,6-bisphosphate (FBP) (to fructose 6-phosphate (F-6-P)) and AMP in vitro, but this might has no significance in vivo. Glucose-1-phosphate (G-1-P), p-nitrophenyl phosphate (pNPP), and beta-glycerol phosphate (beta-GP) are also good substrates, compared to I-1-P. With much lower efficiency, can also hydrolyze inositol 2-phosphate (I-2-P) and glucose-6-phosphate (G-6-P) in vitro, but not fructose-6-phosphate (F-6-P) and trehalose-6-phosphate (T-6-P). {ECO:0000269|PubMed:16325768, ECO:0000269|PubMed:18454554}..

P9WKJ1

Putative lipoprotein LppO

Protein

Unknown.

P9WK71

Dihydrofolate reductase (EC 1.5.1.3)

Protein

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis..

P9WNX1

Thymidylate kinase (EC 2.7.4.9) (Thymidine monophosphate kinase) (dTMP kinase) (TMPK)

Protein

Catalyzes the reversible phosphorylation of deoxythymidine monophosphate (dTMP) to deoxythymidine diphosphate (dTDP), using ATP as its preferred phosphoryl donor. Situated at the junction of both de novo and salvage pathways of deoxythymidine triphosphate (dTTP) synthesis, is essential for DNA synthesis and cellular growth. Has a broad specificity for nucleoside triphosphates, being highly active with ATP or dATP as phosphate donors, and less active with ITP, GTP, CTP and UTP..

P9WKE1

Putative ATPase Rv3679

Protein

Unknown.

P9WKX5