Probable enoyl-CoA hydratase 1 (EC 4.2.1.17) (N-related protein) (Nodulation protein)

Protein

May be involved in the hydration of fatty acids for production of polyhydroxylalkanoates. {ECO:0000269|PubMed:16963641}..

P9WNP3

3-oxoacyl-[acyl-carrier-protein] synthase 3 (EC 2.3.1.180) (3-oxoacyl-[acyl-carrier-protein] synthase III) (Beta-ketoacyl-ACP synthase III) (KAS III) (MtFabH)

Protein

Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Has some substrate specificity for long chain acyl-CoA such as myristoyl-CoA. Does not use acyl-CoA as primer. Its substrate specificity determines the biosynthesis of mycolic acid fatty acid chain, which is characteristic of mycobacterial cell wall. {ECO:0000269|PubMed:10840036}..

P9WNG3

Glucose-6-phosphate 1-dehydrogenase 2 (G6PD 2) (EC 1.1.1.49)

Protein

Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone. {ECO:0000255|HAMAP-Rule:MF_00966}..

P9WN73

Uncharacterized glycosyltransferase Rv1524 (EC 2.4.-.-)

Protein

Unknown.

P9WN07

Probable protein archease

Protein

Activates the tRNA-splicing ligase complex by facilitating the enzymatic turnover of catalytic subunit RtcB. Acts by promoting the guanylylation of RtcB, a key intermediate step in tRNA ligation. Can also alter the NTP specificity of RtcB such that ATP, dGTP or ITP is used efficiently (By similarity). {ECO:0000250}..

P9WQ03

ESAT-6-like protein EsxW

Protein

Unknown.

P9WNI3

ESX-3 secretion system ATPase EccB3 (EC 3.6.-.-) (ESX conserved component B3) (Type VII secretion system protein EccB3) (T7SS protein EccB3)

Protein

An ATPase (By similarity). Part of the ESX-3 specialized secretion system, which is important for iron and zinc uptake or homeostasis. {ECO:0000250|UniProtKB:P9WNR7, ECO:0000269|PubMed:19684129, ECO:0000269|PubMed:24155985}..

P9WNR3

5'-3' exonuclease (EC 3.1.11.-)

Protein

5'-3' exonuclease acting preferentially on double-stranded DNA. {ECO:0000250}..

P9WNU3

Group 2 truncated hemoglobin GlbO (Hemoglobin-like protein HbO) (Truncated hemoglobin) (trHbO)

Protein

When expressed in E.coli and M.smegmatis, HbO increases oxygen uptake. Membrane vesicles of E.coli carrying HbO show a respiration activity about twice that of membranes without HbO. HbO seems to interact with a terminal oxidase. Therefore, HbO could participate in oxygen/electron-transfer process, suggesting a function related to the facilitation of oxygen transfer during aerobic metabolism of M.tuberculosis..

P9WN23

Elongation factor P (EF-P)

Protein

Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase (By similarity). {ECO:0000250}..

P9WNM3

D-3-phosphoglycerate dehydrogenase (PGDH) (EC 1.1.1.95) (2-oxoglutarate reductase) (EC 1.1.1.399)

Protein

Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate. {ECO:0000250|UniProtKB:P0A9T0}..

P9WNX3

PGL/p-HBAD biosynthesis rhamnosyltransferase (EC 2.4.1.-)

Protein

Catalyzes the transfer of the first rhamnosyl residue on p-hydroxybenzoic acid or phenolphthiocerol derivatives to form, after O-methylation at position 2 of the sugar unit, mono-O-methyl-glycosyl-p-hydroxybenzoic acid derivative (p-HBAD I) and 2-O-methyl-rhamnosyl-phenolphthiocerol dimycocerosate (also called mycoside B) during p-hydroxybenzoic acid derivatives (p-HBAD) and glycosylated phenolphthiocerol dimycocerosates (PGL) biosynthesis. {ECO:0000269|PubMed:15292272}..

P9WN09

Formamidopyrimidine-DNA glycosylase 1 (Fapy-DNA glycosylase 1) (EC 3.2.2.23) (DNA-(apurinic or apyrimidinic site) lyase MutM 1) (AP lyase MutM 1) (EC 4.2.99.18)

Protein

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG) when paired with C, G or T, as well as methyl-faPy (formanidopyrimidine residues) in poly(dG-dC) and spiroiminodihydantoin:C base pairs. Unlike its E.coli ortholog has no activity on 8-oxoG:A. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Cleaves ssDNA containing an AP site. Complements the H(2)O(2) sensitivity of an M.smegmatis fpg disruption mutant; upon expression in M.smegmatis excises 8-oxoG from dsDNA. {ECO:0000269|PubMed:17698424, ECO:0000269|PubMed:19496823, ECO:0000269|PubMed:20031487}..

P9WNC3

Galactan 5-O-arabinofuranosyltransferase (EC 2.4.2.46) (Arabinofuranosyltransferase AftA)

Protein

Involved in the biosynthesis of the arabinogalactan (AG) region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an essential component of the mycobacterial cell wall. Catalyzes the addition of the first key arabinofuranosyl (Araf) residue from the sugar donor decaprenyl-phospho-arabinose (DPA) on the C-5 of a 6-linked galactofuranosyl (Galf) of the galactan domain, thus 'priming' the galactan for further elaboration by other arabinofuranosyltransferases. It is not able to add an Araf residue to a terminal Galf. {ECO:0000269|PubMed:16595677, ECO:0000305|PubMed:18556798}..

P9WN03

Chromosomal replication initiator protein DnaA

Protein

Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids (By similarity). Binds its own promoter. {ECO:0000250}..

P9WNW3

Galactokinase (EC 2.7.1.6) (Galactose kinase)

Protein

Catalyzes the transfer of the gamma-phosphate of ATP to D-galactose to form alpha-D-galactose-1-phosphate (Gal-1-P). {ECO:0000255|HAMAP-Rule:MF_00246}..

P9WN63

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC 1.2.1.12) (NAD-dependent glyceraldehyde-3-phosphate dehydrogenase)

Protein

Catalyzes the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG. {ECO:0000269|PubMed:24161676}..

P9WN83

Ferrochelatase (EC 4.99.1.1) (Heme synthase) (Protoheme ferro-lyase)

Protein

Involved in the biosynthesis of heme. Catalyzes the ferrous insertion into protoporphyrin IX to form protoheme. {ECO:0000269|PubMed:11948160, ECO:0000269|PubMed:15850757}..

P9WNE3

Uncharacterized PPE family protein PPE33

Protein

Unknown.

P9WI03

Probable glycine dehydrogenase (decarboxylating) (EC 1.4.4.2) (Glycine cleavage system P-protein) (Glycine decarboxylase) (Glycine dehydrogenase (aminomethyl-transferring))

Protein

The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein (By similarity). {ECO:0000250}..

P9WN53

ESAT-6-like protein EsxN

Protein

Unknown.

P9WNJ3

Putative asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)

Protein

Unknown.

P9WN33

Uncharacterized protein Rv2715

Protein

Unknown.

P9WNH3

1-deoxy-D-xylulose-5-phosphate synthase (EC 2.2.1.7) (1-deoxyxylulose-5-phosphate synthase) (DXP synthase) (DXPS)

Protein

Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP). {ECO:0000255|HAMAP-Rule:MF_00315}..

P9WNS3

DNA translocase FtsK

Protein

Essential cell division protein that coordinates cell division and chromosome segregation. The N-terminus is involved in assembly of the cell-division machinery. The C-terminus functions as a DNA motor that moves dsDNA in an ATP-dependent manner towards the dif recombination site, which is located within the replication terminus region. Required for activation of the Xer recombinase, allowing activation of chromosome unlinking by recombination (By similarity). {ECO:0000250}..

P9WNA3

Deoxyribose-phosphate aldolase (DERA) (EC 4.1.2.4) (2-deoxy-D-ribose 5-phosphate aldolase) (Phosphodeoxyriboaldolase) (Deoxyriboaldolase)

Protein

Catalyzes a reversible aldol reaction between acetaldehyde and D-glyceraldehyde 3-phosphate to generate 2-deoxy-D-ribose 5-phosphate. {ECO:0000255|HAMAP-Rule:MF_00114}..

P9WP03

Fumarate hydratase class II (Fumarase C) (EC 4.2.1.2) (Aerobic fumarase) (Iron-independent fumarase)

Protein

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate. {ECO:0000255|HAMAP-Rule:MF_00743, ECO:0000305|PubMed:27325754}..

P9WN93

Probable dolichyl-phosphate-mannose--protein mannosyltransferase (EC 2.4.1.109)

Protein

Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins (Probable). Mannosylates an artificial substrate, probably on a Thr residue, upon expression in M.smegmatis. Glycosylation probably requires the Sec-translocation system. {ECO:0000269|PubMed:16081738, ECO:0000305}..

P9WN05

Polyprenol-phosphate-mannose-dependent alpha-(1-2)-phosphatidylinositol pentamannoside mannosyltransferase (EC 2.4.1.-) (Alpha-mannosyltransferase) (Alpha-ManT) (PPM-dependent mannosyltransferase) (Polyprenol-phosphate-mannose alpha-mannosyltransferase) (PPM alpha-mannosyltransferase)

Protein

Catalyzes the alpha-1,2 addition of a mannose residue from polyprenol-phosphate-mannose (PPM) to a monoacyl phosphatidylinositol pentamannoside (AcPIM5) to generate a monoacyl phosphatidylinositol hexamannoside (AcPIM6). {ECO:0000269|PubMed:16803893}..

P9WN01

Uncharacterized protein Rv1360

Protein

Unknown.

P9WM03

Trehalose transport system permease protein SugA

Protein

Part of the ABC transporter complex LpqY-SugA-SugB-SugC, which is highly specific for uptake of trehalose. Involved in the recycling of extracellular trehalose released from trehalose-containing molecules synthesized by M.tuberculosis. Trehalose uptake is essential for virulence. Probably responsible for the translocation of the substrate across the membrane. {ECO:0000269|PubMed:21118978}..

P9WG03

GTPase Der (GTP-binding protein EngA)

Protein

GTPase that plays an essential role in the late steps of ribosome biogenesis. {ECO:0000255|HAMAP-Rule:MF_00195}..

P9WNL3

Sulfite reductase [ferredoxin] (EC 1.8.7.1)

Protein

Catalyzes the reduction of sulfite to sulfide, a step in the biosynthesis of sulfur-containing amino acids and cofactors. {ECO:0000269|PubMed:15917234}..

P9WJ03

Ribonuclease 3 (EC 3.1.26.3) (Ribonuclease III) (RNase III)

Protein

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism (By similarity). {ECO:0000250}..

P9WH03

NADPH-dependent stearoyl-CoA 9-desaturase (EC 1.14.19.n4) (Delta(9)-stearoyl desaturase) (Stearoyl-CoA 9-desaturase (NADPH)) (Stearoyl-CoA Delta(9)-desaturase)

Protein

Is likely involved in the aerobic desaturation system responsible for the synthesis of oleic acid from stearoyl-CoA; oleic acid is a precursor of mycobacterial membrane phospholipids and triglycerides. Catalyzes the conversion of stearoyl-CoA to oleoyl-CoA by introduction of a cis double bond between carbons 9 and 10 of the acyl chain. Requires the electron transfer partner Rv3230c to pass two electrons from NADPH to its active site diiron center. Is also able to catalyze the 9-desaturation of palmitoyl-CoA to palmitoleoyl-CoA. {ECO:0000269|PubMed:14559907, ECO:0000269|PubMed:17087501}..

P9WNZ3

Uncharacterized protein Rv0313

Protein

Unknown.

P9WL03

ESX-3 secretion system protein EccD3 (ESX conserved component D3) (Type VII secretion system protein EccD3) (T7SS protein EccD3)

Protein

Part of the ESX-3 specialized secretion system, which is important for iron and zinc uptake or homeostasis. {ECO:0000269|PubMed:19684129, ECO:0000269|PubMed:24155985}..

P9WNQ3

ESAT-6-like protein EsxH (10 kDa antigen CFP7) (CFP-7) (Low molecular weight protein antigen 7) (Protein TB10.4)

Protein

EsxH, in complex with EsxG, disrupts ESCRT function and impairs host phagosome maturation, thereby promoting intracellular bacterial growth. The complex acts by interacting, via EsxH, with the host hepatocyte growth factor-regulated tyrosine kinase substrate (HGS/HRS), a component of the ESCRT machinery. {ECO:0000269|PubMed:24204276}..

P9WNK3

Uncharacterized methyltransferase Rv0089 (EC 2.1.1.-)

Protein

Unknown.

P9WK03

Uncharacterized oxidoreductase Rv0770 (EC 1.1.-.-)

Protein

Unknown.

P9WNY3

Multifunctional non-homologous end joining DNA repair protein LigD (NHEJ DNA repair protein D) (Mt-Lig) (NHEJ DNA polymerase) [Includes: DNA repair polymerase (Pol) (Polymerase/primase); 3'-phosphoesterase (3'-ribonuclease/3'-phosphatase) (PE); DNA ligase (Lig) (EC 6.5.1.1) (Polydeoxyribonucleotide synthase [ATP])]

Protein

With Ku forms a non-homologous end joining (NHEJ) repair enzyme which repairs DNA double-strand breaks (DSB) with reduced fidelity. Recognizes, processes and reseals DSBs, including repairs on incompatible DSB which require 3'-resection, gap filling and ligation. Anneals the 3' overhanging strands from opposing breaks to form a gapped intermediate, which then can be extended in trans by using the termini as primers for extension of the annealed break. Binds to the recessed 5'-phosphate moiety of the downstream DNA strand forming a stable synaptic complex even when the 3'-protruding ends of the template DNA strands are not complementary. Has numerous activites; gap filling copies the template strand, and prefers a 5'-phosphate in the gap and rNTPS (PubMed:17174332, PubMed:17947582), DNA-directed DNA or RNA polymerase on 5'-overhangs, terminal transferase (extending ssDNA or blunt dsDNA in a non-templated fashion, preferentially with rNTPs), DNA-dependent RNA primase (synthesizes short RNAs on unprimed closed ssDNA) and 3'- to 5'-exonuclease on ssDNA (PubMed:15499016). Isolated Pol domain (and presumably the holoenzyme) is able to form complexes between 2 noncompatible protruding 3'-ends DNA ends via microhomologous DNA strands, in a end-bridging function to which it adds a templated nucleotide (PubMed:17947582). Minimal primer length is 2 nucleotides (PubMed:21255731). {ECO:0000269|PubMed:15499016, ECO:0000269|PubMed:17174332, ECO:0000269|PubMed:17947582, ECO:0000269|PubMed:21255731}.; FUNCTION: The preference of the polymerase domain for rNTPs over dNTPs may be advantageous in dormant cells, where the dNTP pool is limiting.; FUNCTION: In conjunction with endogenous or Mycobacterium phage Omega Ku (AC Q853W0) can reconstitute NHEJ in Saccharomyces cerevisiae..

P9WNV3

Methionyl-tRNA formyltransferase (EC 2.1.2.9)

Protein

Attaches a formyl group to the free amino group of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus. {ECO:0000255|HAMAP-Rule:MF_00182}..

P9WND3

DNA polymerase IV 1 (Pol IV 1) (EC 2.7.7.7)

Protein

Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII (By similarity). {ECO:0000250}..

P9WNT3

Glucose-1-phosphate adenylyltransferase (EC 2.7.7.27) (ADP-glucose pyrophosphorylase) (ADPGlc PPase) (ADP-glucose synthase)

Protein

Involved in the biosynthesis of ADP-glucose building block required in the biosynthesis of maltose-1-phosphate (M1P) and in the elongation reactions to produce linear alpha-1,4-glucans. Catalyzes the reaction between ATP and alpha-D-glucose 1-phosphate (G1P) to produce pyrophosphate and ADP-Glc. {ECO:0000269|PubMed:18808383, ECO:0000269|PubMed:27513637}..

P9WN43

Probable enoyl-CoA hydratase echA17 (EC 4.2.1.17)

Protein

Could possibly oxidize fatty acids using specific components. {ECO:0000250}..

P9WNN3

Cell surface glycolipoprotein MPT83 (Lipoprotein p23)

Protein

Recombinant, non-modified protein stimulates secretion of cytokines (TNF-alpha, IL-6 and IL-12p40) by mouse macrophage cell lines in a TLR2-dependent fashion, which leads to increased host innate immunity responses against the bacterium (PubMed:22174456). Serves as a strong human and mouse antigen T cell antigen during M.tuberculosis infection, inducing strong IFN-gamma expression (PubMed:22567094). {ECO:0000269|PubMed:22174456, ECO:0000269|PubMed:22567094}..

P9WNF3

Uncharacterized glycosyl hydrolase Rv3401 (EC 3.2.1.-)

Protein

Unknown.

P9WN13

ESX-1 secretion system protein EccCa1 (ESX conserved component Ca1) (Snm1 secretory protein) (Type VII secretion system protein EccCa1) (T7SS protein EccCa1)

Protein

Part of the ESX-1 specialized secretion system, which delivers several virulence factors to host cells during infection, including the key virulence factors EsxA (ESAT-6) and EsxB (CFP-10). {ECO:0000269|PubMed:14557536, ECO:0000269|PubMed:16368961}..

P9WNB3